Regulation of Ca2+/calmodulin-dependent protein kinase from
A multifunctional Ca2+/calmodulin-dependent protein kinase (TcCaM K) was purified and characterized from the cytosol of Trypanosoma cruzi epimastigote forms. Like mammalian CaM KII, TcCaM K has a broad substrate specificity and a similar subunit composition. Western blot analysis revealed that this...
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| Formato: | Capítulo de libro |
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Elsevier
1996
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| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
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| LEADER | 13490caa a22014897a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18916 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205016.0 | ||
| 008 | 190411s1996 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0030155244 | |
| 024 | 7 | |2 cas |a protein kinase (calcium,calmodulin), 97350-82-8; Antibodies, Monoclonal; Antibodies, Protozoan; Ca(2+)-Calmodulin Dependent Protein Kinase, EC 2.7.1.123; Peptides; syntide-2, 108334-68-5 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MBIPD | ||
| 100 | 1 | |a Cruzi, T. | |
| 245 | 1 | 0 | |a Regulation of Ca2+/calmodulin-dependent protein kinase from |
| 260 | |b Elsevier |c 1996 | ||
| 270 | 1 | 0 | |m Téllez-Iñon, Ma.T.; INGEBI, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina; email: tellez@ingebi.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Londesborough, J., Nuutinen, M., Ca2+/ calmodulin-dependent protein kinase in Saccharomyces cerevisiae (1987) FEBS Lett., 219, pp. 249-253 | ||
| 504 | |a Pausch, M.H., Kaim, D., Kunisawa, R., Admon, A., Thorneer, J., Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote (1991) EMBO J., 10, pp. 1511-1522 | ||
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| 504 | |a Kornstein, L.B., Gaiso, M.L., Hammell, R.L., Bartelt, D.C., Cloning and sequence determination of a cDNA encoding Aspergillus nidulans calmodulin-dependent multifunctional protein kinase (1992) Gene, 113, pp. 75-82 | ||
| 504 | |a Ulloa, R.M., Torres, H.N., Ochatt, C.M., Téllez-Iñón, M.T., Ca2+/calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa (1991) Mol. Cell. Biochem., 102, pp. 155-163 | ||
| 504 | |a Martin, R.G., Ames, B.N., A method for determining the sedimentation behavior of enzymes: Application to protein mixtures (1961) J. Biol. Chem., 236, pp. 1372-1379 | ||
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| 504 | |a Tokumitsu, H., Chijiwa, T., Hagiwara, M., Mizutani, A., Teresawa, M., Hidaka, H., KN-62, 1-[N,O-bis (1,5-isoquinolinesulfony1)-N-methyl-l-tyrosyl]-4-phenylpiperazine, a specific inhibitor of Ca2+/calmodulin-dependent protein kinase II (1990) J. Biol. Chem., 265, pp. 4315-4320 | ||
| 504 | |a LeVine, H., Su, J.L., Sahyoun, N.E., A monoclonal antibody against brain calmodulin-dependent protein kinase type II detects putative conformational changes induced by Ca2+-calmodulin (1988) Biochemistry, 27, pp. 6612-6617 | ||
| 504 | |a Cohen, P., Holmes, C.F.B., Tsukitani, Y., Okadaic acid: A new probe for the study of cellular regulation (1990) Trends Biochem. Sci., 15, pp. 98-102 | ||
| 504 | |a Kelly, P.T., Shenolikar, S., Role of autophosphorylation in regulating calmodulin-dependent protein kinases (1987) Methods Enzymol., 139, pp. 690-714 | ||
| 504 | |a Goldenring, J.R., Gonzalez, B., McGuire J.S., Jr., DeLorenzo, R., Purification and characterization of a calmodulin-dependent kinase from rat brain cytosol able to phosphorylate tubulin and microtubule-associated proteins (1983) J. Biol. Chem., 258, pp. 12632-12640 | ||
| 504 | |a Hashimoto, Y., Soderling, T.R., Calcium.calmodulin-dependent protein kinase II and calcium.Phospholipid-dependent protein kinase activities in rat tissues assayed with a synthetic peptide (1987) Arch. Biochem. Biophys., 252, pp. 418-425 | ||
| 504 | |a Hidaka, H., Sasaki, Y., Tanaka, T., Endo, T., Ohno, S., Fujii, Y., Nagata, T., N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride a calmodulin antagonist, inhibits cell proliferation (1981) Proc. Natl. Acad. Sci. USA, 78, pp. 4354-4357 | ||
| 504 | |a Pearson, R.B., Woodgett, J.R., Cohen, P., Kemp, B.E., Substrate specificity of a multifunctional calmodulin-dependent protein kinase (1985) J. Biol. Chem., 260, pp. 14471-14476 | ||
| 504 | |a Qi, D.-F., Turner, R.S., Kuo, J.F., S-100 and other acidic proteins promote Ca2+-independent phosphorylation of protamine catalyzed by a new protein kinase from brain (1984) J. Neurochem., 42, pp. 458-465 | ||
| 504 | |a Sieghart, W., Forn, J., Greengard, P., Ca2+ and cyclic AMP regulate phosphorylation of same two membrane-associated proteins specific to nerve tissue (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 2475-2481 | ||
| 504 | |a Miller, S.G., Kennedy, M.B., Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: A Ca2+-triggered molecular switch (1986) Cell, 44, pp. 861-870 | ||
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| 504 | |a Lai, Y., Nairn, A.C., Gorelick, F., Greengard, P., Ca2+/calmodulin-dependent protein kinase II: Identification of the autophosphorylation sites responsible for generation of Ca2+/calmodulin-independence (1987) Proc. Natl. Acad. Sci., USA, 84, pp. 5710-5714 | ||
| 504 | |a Erondu, N.E., Donelson, J.E., Characterization of trypanosome protein phosphatase 1 and 2A catalytic subunits (1991) Mol. Biochem. Parasitol., 49, pp. 303-314 | ||
| 504 | |a Willmund, R., Mitschlat, H., Schneider, K., Long-term modulation of Ca2+-stimulated autophosphorylation and subcellular distribution of the Ca2+/calmodulin-dependent protein kinase in the brain of Drosophila (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 9789-9793 | ||
| 504 | |a Vieira, M.C.F., De Carvalho, T.U., De Souza, W., Effect of protein kinase inhibitors on the invasion process of macrophages by Trypanosoma cruzi (1994) Biochem. Biophys. Res. Commun., 203, pp. 967-971 | ||
| 520 | 3 | |a A multifunctional Ca2+/calmodulin-dependent protein kinase (TcCaM K) was purified and characterized from the cytosol of Trypanosoma cruzi epimastigote forms. Like mammalian CaM KII, TcCaM K has a broad substrate specificity and a similar subunit composition. Western blot analysis revealed that this TcCaM K possesses two subunits of 50 and 60 kDa, which exhibited autophosphorylating activity. A panel of monoclonal and polyclonal antibodies raised against rat brain CaM KII could also recognize TcCaM K. However, experimental evidence suggests a different conformational arrangement of the TcCaM K subunits. Like its mammalian counterpart, two highly active autonomous, Ca2+-independent, states of TcCaM K can be isolated. These states, caused by high phosphate incorporation, differ only in their extent of Ca2+/CaM-dependence. About 15-20% of the autophosphorylated TcCaM K can be reverted using protein phosphatase 2A, and, consequently, its Ca2+-dependent activity is also partially restored. The situation is somewhat different when the enzyme is linked to the cytoskeleton, as we have previously shown. The membrane-bound form is present only in the native form. Activation increases its protein kinase activity from 5-to 14-fold. In this study, we provide evidence of another form of TcCaM K present in soluble fractions of the parasite that can be isolated in autonomous states. Our results suggest that autophosphorylation of membrane-bound TcCaM K may be responsible for kinase release in a Ca2+/CaM-independent state. These properties of TcCaM K may play an important role in regulating Ca2+-dependent processes in the parasite. |l eng | |
| 593 | |a Inst. Invest. en Ing. Genet. Y Biol., Molec. and Fac. de Cie. Exac. y Nat., UBA, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina | ||
| 593 | |a INGEBI, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina | ||
| 593 | |a Jules Stein Eye Institute, UCLA School of Medicine, 100 Stein Plaza, Los Angeles, CA 90024-7008, United States | ||
| 690 | 1 | 0 | |a /CAM-DEPENDENT PROTEIN KINASE |
| 690 | 1 | 0 | |a CA2+ |
| 690 | 1 | 0 | |a REGULATION |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a MAMMALIA |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a PROTEIN KINASE (CALCIUM,CALMODULIN) |
| 690 | 1 | 0 | |a MONOCLONAL ANTIBODY |
| 690 | 1 | 0 | |a PEPTIDE |
| 690 | 1 | 0 | |a PROTEIN KINASE (CALCIUM,CALMODULIN) |
| 690 | 1 | 0 | |a PROTOZOON ANTIBODY |
| 690 | 1 | 0 | |a SYNTIDE 2 |
| 690 | 1 | 0 | |a SYNTIDE-2 |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a AUTOPHOSPHORYLATION |
| 690 | 1 | 0 | |a CALCIUM TRANSPORT |
| 690 | 1 | 0 | |a ENZYME PURIFICATION |
| 690 | 1 | 0 | |a ENZYME REGULATION |
| 690 | 1 | 0 | |a ENZYME SPECIFICITY |
| 690 | 1 | 0 | |a ENZYME SUBUNIT |
| 690 | 1 | 0 | |a MEMBRANE BINDING |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BIOLOGICAL MODEL |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a IMMUNOLOGY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MOLECULAR GENETICS |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN CONFORMATION |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIBODIES, MONOCLONAL |
| 690 | 1 | 0 | |a ANTIBODIES, PROTOZOAN |
| 690 | 1 | 0 | |a CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MODELS, BIOLOGICAL |
| 690 | 1 | 0 | |a MOLECULAR SEQUENCE DATA |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a PEPTIDES |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN CONFORMATION |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a SUBSTRATE SPECIFICITY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Ogueta, S. | |
| 700 | 1 | |a Intosh, G.M. | |
| 700 | 1 | |a Téllez-Iñon, Ma.T. | |
| 773 | 0 | |d Elsevier, 1996 |g v. 78 |h pp. 171-183 |k n. 1-2 |p Mol. Biochem. Parasitol. |x 01666851 |w (AR-BaUEN)CENRE-6136 |t Molecular and Biochemical Parasitology | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01666851_v78_n1-2_p171_Cruzi |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v78_n1-2_p171_Cruzi |y Registro en la Biblioteca Digital |
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| 963 | |a VARI | ||
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