Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection

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In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescen...

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Detalles Bibliográficos
Autor principal: Vitale, A.A
Otros Autores: Ferrari, C.C, Aldana, H., Affanni, J.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Elsevier 1996
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 cas  |a melatonin, 73-31-4 
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030 |a JCBBE 
100 1 |a Vitale, A.A. 
245 1 0 |a Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection 
260 |b Elsevier  |c 1996 
270 1 0 |m Vitale, A.A.; Proplame-Conicet, Prog. de Plantas Toxicas/Medicinales, Departamento de Quimia Organica, Pabellon 2, 1428 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Lerner, A.B., Case, J.D., Takahashi, Y., Lee, T.H., Mori, W., (1958) J. Am. Chem. Soc., 80, p. 2587 
504 |a Trentini, G.P., De Gaetani, C., Criscuolo, M., (1991) Aging, 3, p. 103 
504 |a Reiter, R.J., (1992) Bioassays, 14, p. 169 
504 |a Trentini, G.P., Genazzani, A.R., Criscuolo, M., (1992) Neuroendocrinology, 56, p. 364 
504 |a Reiter, R.J., (1993) Braz. J. Med. Biol. Res., 26 (11), p. 1141 
504 |a Pierpaoli, W., Regelson, W., (1994) Proc. Natl. Acad. Sci. USA, 91, p. 787 
504 |a Tamarkin, L., Baird, C.J., Almeida, O.F., (1985) Science, 227, p. 714 
504 |a Chu, E.W., Wurtman, R.J., Axelrod, J., (1964) J. Endocrinol., 75, p. 238 
504 |a Ying, S.Y., Greep, R.O., (1973) Endocrinology, 92, p. 333 
504 |a Menendez-Pelaez, A., Reiter, R.J., (1993) J. Pineal Res., 15, p. 59 
504 |a Huether, G., (1993) Experientia, 49, p. 665 
504 |a Ralph, C.L., Lynch, H.J., (1970) Gen. Comp. Endocrinol., 15, p. 334 
504 |a Miller, F.P., Maickel, R.P., (1970) Life Sci., 9, p. 747 
504 |a Greiner, A.C., Chan, S.S., (1978) Science, 199, p. 83 
504 |a Fuller, R.W., Perry, K.W., (1977) Res. Commun. Pathol. Pharmacol., 18, p. 769 
504 |a Mefford, I.N., Barchas, J.D., (1981) J. Chromatogr., 181, p. 187 
504 |a Anderson, G.M., Young, J.G., Batter, D.K., Young, S.N., Cohen, D.J., Shaywitz, B.A., (1981) J. Chromatogr., 223, p. 315 
504 |a Vakkuri, O., Leppaluoto, J., Vuolteenaho, O., (1984) Acta Endocrinol., 106, p. 152 
504 |a Skene, D.J., Smith, I., Arendt, J., (1986) J. Endocrinol., 110, p. 177 
504 |a Yamada, K.Y., Sakamoto, Y., Satoh, T., (1984) Res. Commun. Chem. Pathol. Pharmacol., 46, p. 283 
504 |a Mills, M.H., King, M.G., (1986) J. Chromatogr., 377, p. 350 
504 |a Anderson, G.M., Young, J.G., Cohen, D.J., (1982) J. Chromatogr., 228, p. 155 
504 |a Wakabayashi, H., Shimada, K., (1986) J. Chromatogr., 381, p. 21 
504 |a Chin, J.L., (1988) J. Chromatogr., 428, p. 206 
504 |a Goldman, M.E., Hamm, H., Erickson, C.K., (1980) J. Chromatogr., 190, p. 217 
504 |a Murai, S., Saito, H., Masuda, Y., Itoh, T., (1988) J. Neurochem., 50, p. 473 
504 |a Hernandez, G., Abreu, P., Alonso, R., Calzadilla, C.H., (1990) J. Pineal Res., 8, p. 11 
504 |a Fourtillan, J.B., Gobin, P., Faye, B., (1994) Biol. Mass Spectrom., 23, p. 499 
520 3 |a In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Thanks are due to Conicet (Argentina) and Uni-versidad de Buenos Aires (Argentina) for financial support. Two of us (C.F. and H.A.) also thank Conicet (Argentina) for research fellowships. 
593 |a Proplame-Conicet, Prog. Plantas Toxicas y Medicinales, Ciudad Universitaria, 1428 Buenos Aires, Argentina 
593 |a Ineuci-Conicet, Instituto de Neurociencias, Ciudad Universitaria, 1428 Buenos Aires, Argentina 
690 1 0 |a MELATONIN 
690 1 0 |a MELATONIN 
690 1 0 |a ANIMAL TISSUE 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DRUG DETERMINATION 
690 1 0 |a FLUOROMETRY 
690 1 0 |a HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 
690 1 0 |a MALE 
690 1 0 |a NONHUMAN 
690 1 0 |a PINEAL BODY 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a TECHNIQUE 
700 1 |a Ferrari, C.C. 
700 1 |a Aldana, H. 
700 1 |a Affanni, J.M. 
773 0 |d Elsevier, 1996  |g v. 681  |h pp. 381-384  |k n. 2  |p J. CHROMATOGR. B BIOMED. APPL.  |x 15726495  |t Journal of Chromatography B: Biomedical Applications 
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856 4 0 |u https://doi.org/10.1016/0378-4347(96)00051-5  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_15726495_v681_n2_p381_Vitale  |y Handle 
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961 |a paper_15726495_v681_n2_p381_Vitale  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
999 |c 79776 

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