Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solu...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1974
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 08704caa a22012617a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18701 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230420114217.0 | ||
| 008 | 190411s1974 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0015973906 | |
| 024 | 7 | |2 cas |a lipid, 66455-18-3; magnesium, 7439-95-4; manganese, 16397-91-4, 7439-96-5; uridine diphosphate glucose, 133-89-1; Borohydrides; Butanols; Carbon Radioisotopes; Galactose, 26566-61-0; Glucose, 50-99-7; Hexokinase, EC 2.7.1.1; Lipids; Magnesium, 7439-95-4; Manganese, 7439-96-5; Phosphoglucomutase, EC 5.4.2.2; Phosphorus Radioisotopes; Polysaccharides, Bacterial; Uridine Diphosphate Sugars | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a García, R.C. | |
| 245 | 1 | 0 | |a Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| 260 | |c 1974 | ||
| 270 | 1 | 0 | |m García, R.C.; Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Obligado, Buenos Sires, 2490, Argentina |
| 504 | |a Lennarz, W.J., Scher, M.G., (1972) Biochim. Biophys. Acta, 265, p. 417 | ||
| 504 | |a Wright, A., Dankert, M., Fennessey, P., Robbins, P.W., (1967) Proc. Natl. Acad. Sci. U. S. A., 57, p. 235 | ||
| 504 | |a Weiner, I.M., Higuchi, T., Rothfield, L., Saltmarsh‐Andrew, M., Osborn, M.J., Horecker, B.L., (1965) Proc. Natl. Acad. Sci. U.S.A., 54, p. 228 | ||
| 504 | |a Higashi, Y., Strominger, J.L., Sweeley, C.C., (1967) Proc. Natl Acad. Sci. U. S. A., 57, p. 1878 | ||
| 504 | |a Troy, F.A., Frerman, F.E., Heath, E.C., (1971) J. Biol. Chem., 246, p. 118 | ||
| 504 | |a Wright, A., (1971) J. Bacteriol., 105, p. 927 | ||
| 504 | |a Nikaido, K., Nikaido, H., (1971) J. Biol. Chem., 246, p. 3912 | ||
| 504 | |a Scher, M.G., Lennarz, W.J., Sweeley, C., (1968) Proc. Natl Acad. Sci. U. S. A., 59, p. 1313 | ||
| 504 | |a Glaser, L., The synthesis of cellulose in cell-free extracts of Acetobacter xylinum. (1958) J Biol Chem, 232, p. 627 | ||
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| 504 | |a Khan, A.W., Colvin, J.R., (1961) Science (Wash. D. C.), 133, p. 2014 | ||
| 504 | |a Reference deleted; Dankert, M., Recondo, E., (1969), p. 175. , Abstr. Commun. 6th Meet. Fed. Eur. Biochem. Soc; Dankert, M., García, R.C., Recondo, E., (1972) The Biochemistry of the Glycosidic Linkage, p. 199. , Piras, R., Pontis, H. G., eds, p., Academic Press, Academic Press, New York | ||
| 504 | |a Wright, A., Robbins, P.W., (1965) Biochim. Biophys. Acta, 104, p. 594 | ||
| 504 | |a Thomas, J.A., Keith, K., Schlender, K., Larner, J., (1968) Anal. Biochem., 25, p. 486 | ||
| 504 | |a Albrecht, G.J., Bass, S.T., Seifert, L.L., Hansen, R.G., (1966) J. Biol. Chem., 241, p. 2968 | ||
| 504 | |a Kunitz, M., (1952) J. Gen. Physiol, 35, p. 423 | ||
| 504 | |a Kunitz, M., (1971) Arch. Biochem. Biophys., 92, p. 270 | ||
| 504 | |a Fischer, E.M., Krebs, E.G., (1962) Methods Enzymol, 5, p. 369 | ||
| 504 | |a Dankert, M., Wright, A., Kelley, W.S., Bobbins, P.W., (1966) Arch. Biochem. Biophys., 116, p. 425 | ||
| 504 | |a Wilson, D., (1965) Anal Biochem., 10, p. 472 | ||
| 504 | |a Paladini, A.C., Leloir, L.F., (1952) Biochem. J., 51, p. 426 | ||
| 504 | |a Clancy, M.J., (1960), p. 4213. , J. Chem. Soc; Hestrin, S., Schramm, M., (1954) Biochem. J., 58, p. 345 | ||
| 504 | |a Robbins, P.W., Wright, A., Bellows, J.L., (1964) Proc. Natl Acad. Sci. U. S. A., 52, p. 1302 | ||
| 504 | |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Bandall, R.J., (1951) J. Biol. Chem., 193, p. 265 | ||
| 504 | |a Dawson, R.M.C., (1954) Biochim. Biophys. Acta, 14, p. 374 | ||
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| 504 | |a Behrens, N.H., Leloir, L.F., (1970) Proc. Natl. Acad. Sci. U. S. A., 66, p. 153 | ||
| 504 | |a Maxwell, E.S., Kurahashi, K., Kalckar, H.M., (1962) Methods Enzymol, 5, p. 174 | ||
| 504 | |a Jeanes, A., Wise, C.S., Dimler, B.J., (1951) Anal Chem., 23, p. 415 | ||
| 504 | |a Tung, K.K., Nordin, J.H., (1968) Biochim. Biophys. Acta, 158, p. 154 | ||
| 504 | |a Markham, R., Smith, J.D., (1952) Biochem. J., 52, p. 552 | ||
| 504 | |a Aso, K., Hamada, S., (1955) Tôkuku J. Agric. Res., 5, p. 317 | ||
| 504 | |a Bourne, J., Hutson, D.H., Weigel, H., (1961), p. 35. , J. Chem. Soc; Trevelyan, W.E., Procter, D.P., Harrison, J.S., (1950) Nature (Lond.), 166, p. 444 | ||
| 504 | |a Burrows, S., Grylls, F.S.M., Harrison, J.S., (1952) Nature (Lond.), 170, p. 800 | ||
| 504 | |a Bray, G.A., (1960) Anal Biochem., 1, p. 279 | ||
| 504 | |a Leloir, L.F., Cardini, C.E., (1963) Comprehensive Biochemistry, 5, p. 133. , Florkin, M., Stotz, E. H., eds, vol., Elsevier, Amsterdam | ||
| 504 | |a Piras, R., (1963) Arch. Biochem. Biophys., 103, p. 291 | ||
| 504 | |a Tanner, W., (1969) Biochem. Biophys. Res. Commun., 35, p. 144 | ||
| 504 | |a Umbreit, J.N., Strominger, J.L., (1972) Proc. Natl. Acad. Sci. U. S. A., 69, p. 1972 | ||
| 504 | |a Sutherland, I.W., Norval, M., (1970) Biochem. J., 120, p. 567 | ||
| 504 | |a Scher, M.G., Lennarz, W.J., (1969) J. Biol. Chem., 244, p. 2777 | ||
| 504 | |a Martell, A., (1964) Stability Constants of Metal‐Ion Complexes, p. 638. , 2nd edn, The Chemical Society, London | ||
| 506 | |2 openaire | ||
| 520 | 3 | |a The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solubilized by hot alkali, leaving an insoluble fraction. No incorporation was observed in the absence of magnesium ions. Mn2+ could only partially replace Mg2+, and had an inhibitory effect when both ions were present. Analysis of the butanol‐soluble fraction permitted the isolation of at least three components characterized as: lipid‐diphosphate α‐glucose, lipid‐diphosphate α‐cellobiose and lipid‐monophosphate β‐galactose. In the presence of [β‐32P]UDP‐glucose the system incorporated radioactivity only into the diphosphate derivatives, indicating the transfer of a α‐glucose 1‐phosphate group from UDP‐glucose. Experiments with doubly labelled substrate confirmed these results. The role these compounds might play in polysaccharide biosynthesis is discussed. Copyright © 1974, Wiley Blackwell. All rights reserved |l eng | |
| 593 | |a Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Obligado, Buenos Sires, 2490, Argentina | ||
| 593 | |a Departamento de Química Biologica, Facultad de Ciencas Exactas y Naturales, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a LIPID |
| 690 | 1 | 0 | |a MAGNESIUM |
| 690 | 1 | 0 | |a MANGANESE |
| 690 | 1 | 0 | |a POLYSACCHARIDE |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE GLUCOSE |
| 690 | 1 | 0 | |a ACETOBACTER XYLINUM |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a MICROORGANISM |
| 690 | 1 | 0 | |a THEORETICAL STUDY |
| 690 | 1 | 0 | |a ACETOBACTER |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BOROHYDRIDES |
| 690 | 1 | 0 | |a BUTANOLS |
| 690 | 1 | 0 | |a CARBON RADIOISOTOPES |
| 690 | 1 | 0 | |a CELL DIVISION |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, DEAE-CELLULOSE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, ION EXCHANGE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, PAPER |
| 690 | 1 | 0 | |a ELECTROPHORESIS, PAPER |
| 690 | 1 | 0 | |a GALACTOSE |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a HEXOKINASE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LIPIDS |
| 690 | 1 | 0 | |a MAGNESIUM |
| 690 | 1 | 0 | |a MANGANESE |
| 690 | 1 | 0 | |a MUSCLES |
| 690 | 1 | 0 | |a PHOSPHOGLUCOMUTASE |
| 690 | 1 | 0 | |a PHOSPHORUS RADIOISOTOPES |
| 690 | 1 | 0 | |a POLYSACCHARIDES, BACTERIAL |
| 690 | 1 | 0 | |a RABBITS |
| 690 | 1 | 0 | |a SACCHAROMYCES CEREVISIAE |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a TIME FACTORS |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE SUGARS |
| 700 | 1 | |a Recondo, E. | |
| 700 | 1 | |a Dankert, M. | |
| 773 | 0 | |d 1974 |g v. 43 |h pp. 93-105 |k n. 1 |p Eur. J. Biochem. |x 00142956 |w (AR-BaUEN)CENRE-3060 |t European Journal of Biochemistry | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0015973906&doi=10.1111%2fj.1432-1033.1974.tb03389.x&partnerID=40&md5=27dc240505c8df7cf79f392b0e18f773 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1111/j.1432-1033.1974.tb03389.x |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v43_n1_p93_Garcia |y Registro en la Biblioteca Digital |
| 961 | |a paper_00142956_v43_n1_p93_Garcia |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 79654 | ||