Assay of dolichol monophosphate and dolichol diphosphate phosphatases: Thin-layer chromatography in a nonequilibrated system
A simple and rapid method for determining the activity of dolichol monophosphate phosphatase and dolichol diphosphate phosphatase is reported. This procedure is based on the separation of the substrates, [3H]dolichol monophosphate and [3H]dolichol diphosphate, from their reaction products, [3H]dolic...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1981
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04349caa a22006017a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18486 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204951.0 | ||
| 008 | 190411s1981 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0019795518 | |
| 024 | 7 | |2 cas |a dolichol phosphate, 12698-55-4, 34457-14-2; phosphatase, 9013-05-2; dolichyl-phosphatase, EC 3.1.3.51; dolichyldiphosphatase, EC 3.6.1.43; Phosphoric Monoester Hydrolases, EC 3.1.3; Pyrophosphatases, EC 3.6.1.-; Tritium, 10028-17-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ANBCA | ||
| 100 | 1 | |a Boscoboinik, D.O. | |
| 245 | 1 | 0 | |a Assay of dolichol monophosphate and dolichol diphosphate phosphatases: Thin-layer chromatography in a nonequilibrated system |
| 260 | |c 1981 | ||
| 270 | 1 | 0 | |m Boscoboinik, D.O.; Instituto de Investigaciones Bioquímicas Fundación Campomar. Obligado 2490, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Wedgwood, Strominger, (1980) J. Biol. Chem, 255, pp. 1120-1123 | ||
| 504 | |a Idoyaga-Vargas, Belocopitow, Mentaberry, Carminatti, (1980) FEBS Lett, 112, pp. 63-66 | ||
| 504 | |a Parodi, Mordoh, Krisman, Leloir, (1969) Arch. Biochem. Biophys, 132, pp. 111-117 | ||
| 504 | |a Folch, Lees, Sloane Stanley, (1957) J. Biol. Chem, 226, pp. 497-509 | ||
| 504 | |a Truter, (1964) J. Chromatogr, 14, pp. 57-61 | ||
| 504 | |a Libbey, Day, Reverse phase thin-layer chromatography of 2,4-dinitrophenylhydrazones of n-alkanals and n-alkan-2-ones (1964) Journal of Chromatography A, 14, pp. 275-279 | ||
| 504 | |a Van Den Eijden, Continuous flow thin-layer chromatography: A new technique (1974) Analytical Biochemistry, 57, pp. 321-322 | ||
| 520 | 3 | |a A simple and rapid method for determining the activity of dolichol monophosphate phosphatase and dolichol diphosphate phosphatase is reported. This procedure is based on the separation of the substrates, [3H]dolichol monophosphate and [3H]dolichol diphosphate, from their reaction products, [3H]dolichol and [3H]dolichol monophosphate, by thin-layer chromatography on silica gel plastic plates. The chromatography chamber is of a nonconventional type, as it has a thin slot from which the last millimeter of the plate emerges. This special chamber allows the solvent to evaporate as it reaches the top of the plate. As a consequence, the mobile compounds tend to concentrate at the edge of the plate. The dolichol monophosphate phosphatase and dolichol diphosphate phosphatase substrates, which are more negatively charged than the corresponding reaction products, remain at the origin whereas the products migrate to the edge of the plate when appropriate solvents are used. The measurement of the radioactivity present at both the origin and the edge of the plate allows rapid estimation of the enzymatic activities. © 1981. |l eng | |
| 593 | |a Instituto de Investigaciones Bioquímicas Fundación Campomar. Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a DOLICHOL PHOSPHATE |
| 690 | 1 | 0 | |a PHOSPHATASE |
| 690 | 1 | 0 | |a DOLICHOL DIPHOSPHATE |
| 690 | 1 | 0 | |a ENZYME ASSAY |
| 690 | 1 | 0 | |a METHODOLOGY |
| 690 | 1 | 0 | |a THIN LAYER CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, THIN LAYER |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MICROSOMES, LIVER |
| 690 | 1 | 0 | |a PHOSPHORIC MONOESTER HYDROLASES |
| 690 | 1 | 0 | |a PYROPHOSPHATASES |
| 690 | 1 | 0 | |a RADIOISOTOPE DILUTION TECHNIQUE |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRITIUM |
| 700 | 1 | |a Belocopitow, E. | |
| 773 | 0 | |d 1981 |g v. 114 |h pp. 42-45 |k n. 1 |p Anal. Biochem. |x 00032697 |w (AR-BaUEN)CENRE-163 |t Analytical Biochemistry | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019795518&doi=10.1016%2f0003-2697%2881%2990448-6&partnerID=40&md5=1f75a263d0a0437944150d46a1653769 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0003-2697(81)90448-6 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00032697_v114_n1_p42_Boscoboinik |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v114_n1_p42_Boscoboinik |y Registro en la Biblioteca Digital |
| 961 | |a paper_00032697_v114_n1_p42_Boscoboinik |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 79439 | ||