Melatonin increases cGMP and decreases cAMP levels in rat medial basal hypothalamus in vitro

Measurement of cyclic nucleotide accumulation in rat medial basal hypothalamus (MBH) incubated in vitro with methoxyindoles indicated that melatonin (10-8 M or greater) increased significantly cGMP and depressed cAMP levels. Only the effect on MBH cAMP was shared by 5-methoxytryptophol which exhibit...

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Autor principal: Vacas, M.I
Otros Autores: Keller Sarmiento, M.I, Cardinali, D.P
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1981
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 cas  |a 5 methoxytryptophol, 712-09-4; 6 hydroxymelatonin, 2208-41-5; cyclic AMP, 60-92-4; cyclic GMP, 7665-99-8; melatonin, 73-31-4; Cyclic AMP, 60-92-4; Cyclic GMP, 7665-99-8; Melatonin, 73-31-4 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a BRREA 
100 1 |a Vacas, M.I. 
245 1 0 |a Melatonin increases cGMP and decreases cAMP levels in rat medial basal hypothalamus in vitro 
260 |c 1981 
270 1 0 |m Vacas, M.I.; Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), V. de Obligado 2490, 1428 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Anton-Tay, Pineal-brain relationships (1971) The Pineal Gland, pp. 213-227. , G.E.W. Wolstenholme, J. Knight, Churchill Livingstone, London 
504 |a Anton-Tay, Chou, Anton, Wurtman, Brain serotonin concentration: elevation following intraperitoneal administration of melatonin (1968) Science, 162, pp. 277-278 
504 |a Brown, Albano, Ekins, Sgherzi, Tampion, A simple and sensitive saturation assay method for the measurement of adenosine-3′,5′-cyclic monophosphate (1971) Biochem. J., 121, pp. 561-562 
504 |a Cardinali, D. P., Melatonin. A mammalian pineal hormone,Endocrine Rev., in press; Cardinali, Freire, Melatonin effects on brain. Interaction with microtubule protein, inhibition of fast axoplasmic flow and induction of crystaloid and tubular formations in the hypothalamus (1975) Molec. Cell. Endocr., 2, pp. 317-330 
504 |a Cardinali, Nagle, Freire, Rosner, Effects of melatonin on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus (1975) Neuroendocrinology, 18, pp. 72-85 
504 |a Cardinali, Nagle, Rosner, Incorporation of3H-leucine into proteins in the hypothalamus and the anterior hypophysis. Effects of pinealectomy, superior cervical sympathectomy and continuous exposure to light or darkness (1974) Neuroendocrinology, 16, pp. 74-83 
504 |a Cardinali, Ritta, Fuentes, Gimeno, Gimeno, Prostaglandin E release by rat medial basal hypothalamus in vitro. Inhibition by melatonin at submicromolar concentrations (1980) Europ. J. Pharmacol., 67, pp. 151-153 
504 |a Cardinali, Vacas, Boyer, Specific binding of melatonin in bovine brain (1979) Endocrinology, 105, pp. 437-441 
504 |a Cohen, Roselle, Chabner, Schmidt, Lippman, Evidence for a cytoplasmic melatonin receptor (1978) Nature (Lond.), 274, pp. 894-895 
504 |a Flaugh, Crowell, Clemens, Sawyer, Synthesis and evaluation of the antiovulatory activity of a variety of melatonin analogues (1979) J. Med. Chem., 22, pp. 63-64 
504 |a Greengard, (1978) Cyclic Nucleotides, Phosphorylated Proteins, and Neuronal Function. Distinguished Lecture Series of the Society of General Physiologists, 1, p. 124. , Raven Press, New York 
504 |a Kano, Miyachi, Direct action of melatonin on testosterone and cyclic GMP production using rat testis tissue in vitro (1976) Biochem. Biophys. Res. Commun., 72, pp. 969-975 
504 |a Kao, Weisz, Release of gonadotrophin-releasing hormone (Gn-RH) from isolated, perifused medial basal hypothalamus by melatonin (1977) Endocrinology, 100, pp. 1723-1726 
504 |a Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275 
504 |a Martin, Kirk, Klein, Effects of 6-hydroxy-, 6-fluoro-, and 4, 6-difluoromelatonin on the in vitro pituitary response to luteinizing hormone-releasing hormone (1980) Endocrinology, 106, pp. 398-401 
504 |a Mullen, Leone, Hooper, Smith, Silman, Finnie, Carter, Linsell, Pineal 5-methoxy tryptophol in man (1979) Psychoneuroendocrinology, 2, pp. 117-126 
504 |a Ojeda, Negro-Vilar, McCann, Release of prostaglandin Es by hypothalamic tissue: evidence for their involvement in catecholamine-induced luteinizing hormone-releasing hormone release (1979) Endocrinology, 104, pp. 617-624 
504 |a Reiter, The pineal and its hormones in the control of reproduction in mammals (1980) Endocrine Rev., 1, pp. 109-131 
504 |a Rudman, Injection of melatonin into cisterna magna increases concentration of 3′,5′-cyclic guanosine monophosphate in cerebrospinal fluid (1976) Neuroendocrinology, 20, pp. 235-242 
504 |a Sandler, Clyman, Manganiello, Vaughan, The effect of serotonin (5-hydroxytryptamine) and derivatives on guanosine 3′,5′-monophosphate in human monocytes (1975) J. clin. Invest., 55, pp. 431-435 
504 |a Vesely, Melatonin enhances guanylate cyclase activity in a variety of tissues (1981) Molecular and Cellular Biochemistry, 35, pp. 55-58 
520 3 |a Measurement of cyclic nucleotide accumulation in rat medial basal hypothalamus (MBH) incubated in vitro with methoxyindoles indicated that melatonin (10-8 M or greater) increased significantly cGMP and depressed cAMP levels. Only the effect on MBH cAMP was shared by 5-methoxytryptophol which exhibited a greater activity than melatonin. 6-Fluromelatonin (10-7 M) increased cGMP, whereas the effect of 6-hydroxymelatonin was not significant. Both 6-fluoro- and 6-hydroxymelatonin (10-5 M) depressed MBH cAMP accumulation while only 6-fluoromelatonin affected it at 10-7 M concentrations. These data suggest that melatonin and 5-methoxytryptophol affect differently cyclic nucleotide content of MBH at physiological concentrations. © 1981.  |l eng 
536 |a Detalles de la financiación: The authors wish to thank Dr. Michael E. Flaugh, Lilly Research Laboratories, Indianapolis, IN, for kindly supplying 6-fluoromelatonin. This study was supported by Grant 6638 from the Consejo Nacional de Investi-gaciones Cientificas y T6cnicas (CONICET), Argentina. M.I.V. and D.P.C. are Established Investigators, CONICET. 
593 |a Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), V. de Obligado 2490, 1428 Buenos Aires, Argentina 
690 1 0 |a 5-METHOXYTRYPTOPHOL 
690 1 0 |a CAMP 
690 1 0 |a CGMP 
690 1 0 |a MEDIAL BASAL HYPOTHALAMUS 
690 1 0 |a MELATONIN 
690 1 0 |a PINEAL GLAND 
690 1 0 |a 5 METHOXYTRYPTOPHOL 
690 1 0 |a 6 HYDROXYMELATONIN 
690 1 0 |a CYCLIC AMP 
690 1 0 |a CYCLIC GMP 
690 1 0 |a MELATONIN 
690 1 0 |a 6 FLUOROMELATONIN 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a CENTRAL NERVOUS SYSTEM 
690 1 0 |a HYPOTHALAMUS 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a NERVOUS SYSTEM 
690 1 0 |a PINEAL BODY 
690 1 0 |a RAT 
690 1 0 |a ANIMAL 
690 1 0 |a CYCLIC AMP 
690 1 0 |a CYCLIC GMP 
690 1 0 |a DOSE-RESPONSE RELATIONSHIP, DRUG 
690 1 0 |a HYPOTHALAMUS 
690 1 0 |a MALE 
690 1 0 |a MELATONIN 
690 1 0 |a RATS 
690 1 0 |a RATS, INBRED STRAINS 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
700 1 |a Keller Sarmiento, M.I. 
700 1 |a Cardinali, D.P. 
773 0 |d 1981  |g v. 225  |h pp. 207-211  |k n. 1  |p Brain Res.  |x 00068993  |w (AR-BaUEN)CENRE-4052  |t Brain Research 
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