Androgens control androgen-binding sites in rat epididymis
Previous results suggested that the number of androgen-binding sites in rat epididymis was controlled by androgens. Using an exchange technique for receptor determination, we now report that cytoplasmic receptor number decreased to about half the control value after 4 days of castration and reached...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1983
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 07696caa a22009137a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18370 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204944.0 | ||
| 008 | 190411s1983 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0020508019 | |
| 024 | 7 | |2 cas |a testosterone propionate, 57-85-2; Androgens; Estrenes; Metribolone, 965-93-5; Peptide Hydrolases, EC 3.4; Receptors, Androgen; Receptors, Steroid; Testosterone, 57-85-2 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Tezon, J.G. | |
| 245 | 1 | 0 | |a Androgens control androgen-binding sites in rat epididymis |
| 260 | |c 1983 | ||
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Podestä, E.J., Calandra, R.S., Rivarola, M.A., Blaquier, J.A., The effect of castration and testosterone replacement on specific proteins and androgen levels of the rat epididymis (1975) Endocrinology, 97, p. 399 | ||
| 504 | |a Calandra, R.S., Blaquier, J.A., Del Castillo, E.J., Rivarola, M.A., Androgen dependency of the androgen receptor in rat epididymis (1975) Biochem Biophys Res Commun, 67, p. 97 | ||
| 504 | |a Pujol, A., Bayard, F., Androgen receptors in the rat epididymis and their hormonal control (1979) J Reprod Fertil, 56, p. 217 | ||
| 504 | |a Calandra, R.S., Purvis, K., Attramadal, A., Hansson, V., Androgen receptors in rat epididymis do not disappear after castration (1977) J Steroid Biochem, 8, p. 1205 | ||
| 504 | |a Tezon, J.G., Vazquez, M.H., Blaquier, J.A., Androgen determined subcellular distribution of receptor in rat epididymis. 5a-DHT induced nuclear translocation is blocked by antiandrogens (1982) Endocrinology, 111, p. 2039 | ||
| 504 | |a Blaquier, J.A., Selective uptake and metabolism of androgens by rat epididymis. The presence of a cytoplasmic receptor (1971) Biochem Biophys Res Commun, 45, p. 1076 | ||
| 504 | |a Fell, H.B., Dingle, J.T., Studies on the mode of action of excess vitamin A 6. Lysosomal protease and the degradation of cartilage matrix (1963) Biochem J, 87, p. 403 | ||
| 504 | |a Lowry, O., Rosebrough, N.S., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, p. 265 | ||
| 504 | |a Burton, K., A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid (1956) Biochem J, 62, p. 315 | ||
| 504 | |a Blaquier, J.A., Breger, D., The in vitro effects of androgens on RNA synthesis by cultured rat epididymal tubules (1974) Endocr Res Commun, 1, p. 247 | ||
| 504 | |a Mori, H., Christensen, A.K., Morphometrie analysis of Leydig cells in the normal rat testis (1980) J Cell Biol, 84, p. 340 | ||
| 504 | |a Tindall, D.J., Hansson, V., Sar, M., Stumpf, W.E., Frech, F.S., Nayfeh, S.N., Further studies on the accumulation and binding of androgens in rat epididymis (1974) Endocrinology, 95, p. 1119 | ||
| 504 | |a De Larminat, M.A., Monsalve, A., Charreau, E.H., Calandra, R.S., Blaquier, J.A., Hormonal regulation of 5a-reductase activity in rat epididymis (1978) J Endocrinol, 79, p. 157 | ||
| 504 | |a Cameo, M.S., Blaquier, J.A., Androgen-controlled specific proteins in rat epididymis (1976) J Endocrinol, 69, p. 47 | ||
| 504 | |a Parker, M.G., Scrace, G.T., Mainwaring, W., Testosterone regulates the synthesis of major proteins in rat ventral prostate (1978) Biochem J, 170, p. 115 | ||
| 504 | |a Lesser, B., Bruchovsky, N., The effects of testosterone, 5a-dihydrotestosterone and adenosine 3'-5' monophosphate on cell proliferation and differentiation in rat prostate (1973) Biochim Biophys Acta, 308, p. 426 | ||
| 504 | |a Clark, J.H., Hardin, J.W., Upchurch, S., Eriksson H1978 Heterogeneity of estrogen binding sites in the cytosol of rat uterus J Biol Chem, 253, p. 7630 | ||
| 504 | |a Maclusky, N.J., McEwen, B.S., Progestin receptor in rat brain: Distribution and properties of cytoplasmic progestin-binding sites (1980) Endocrinology, 106, p. 192 | ||
| 520 | 3 | |a Previous results suggested that the number of androgen-binding sites in rat epididymis was controlled by androgens. Using an exchange technique for receptor determination, we now report that cytoplasmic receptor number decreased to about half the control value after 4 days of castration and reached its lowest level (30% of the control value) after 12 days of castration. The Kd for [3H]methyltrienolone for cytoplasmic receptor varied from 2.8 × 10−9 M in controls to 0.6 × 10−9 M (P < 0.001) after 6 or more days of castration. Nuclear binding sites were reduced to 17% of the control value from the second day of castration on, but no change in the affinity for methyltrienolone was noted. The administration of testosterone propionate (400 μg/day) to rats castrated for 20 days significantly increased the number of nuclear and cytoplasmic binding sites from the second and third days of treatment, respectively. Labeled thymidine incorporation into DNA rose significantly after a 4-day latency period. The proportion of total binding sites capable of translocation into the nucleus was elevated during the early phase (2–4 days) of androgen treatment. These results also suggest the heterogeneity of the cytoplasmic binding site population. Control epididymides were composed of 61.5% receptor-containing epithelial cells, and this proportion was significantly reduced to 53.2% after 20 days of castration. Androgen administration elevated this percentage after an 8-day latency period. Proteolytic activity in cytosol was increased over control values after castration (for 20 days) and until the fourth day after the onset of androgen treatment. However, this activity does not seem to be an important factor in the decrease in binding sites caused by orchidectomy. © 1983 by The Endocrine Society. |l eng | |
| 593 | |a Institute de Biologia y Medicina Experimental, Laboratorio de Esteroides, Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 690 | 1 | 0 | |a ANDROGEN |
| 690 | 1 | 0 | |a CELL NUCLEUS RECEPTOR |
| 690 | 1 | 0 | |a CYTOSOL RECEPTOR |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a TESTOSTERONE PROPIONATE |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a CASTRATION |
| 690 | 1 | 0 | |a DRUG EFFICACY |
| 690 | 1 | 0 | |a DRUG RECEPTOR BINDING |
| 690 | 1 | 0 | |a ENDOCRINE SYSTEM |
| 690 | 1 | 0 | |a EPIDIDYMIS |
| 690 | 1 | 0 | |a MALE GENITAL SYSTEM |
| 690 | 1 | 0 | |a METHYLTRIENOLONE H 3 |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PHARMACOKINETICS |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a THYMIDINE H 3 |
| 690 | 1 | 0 | |a ANDROGENS |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CASTRATION |
| 690 | 1 | 0 | |a CELL NUCLEUS |
| 690 | 1 | 0 | |a CYTOPLASM |
| 690 | 1 | 0 | |a EPIDIDYMIS |
| 690 | 1 | 0 | |a ESTRENES |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a METRIBOLONE |
| 690 | 1 | 0 | |a PEPTIDE HYDROLASES |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, INBRED STRAINS |
| 690 | 1 | 0 | |a RECEPTORS, ANDROGEN |
| 690 | 1 | 0 | |a RECEPTORS, STEROID |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 700 | 1 | |a Blaquier, J.A. | |
| 773 | 0 | |d 1983 |g v. 113 |h pp. 1025-1030 |k n. 3 |p Endocrinology |x 00137227 |w (AR-BaUEN)CENRE-4573 |t Endocrinology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020508019&doi=10.1210%2fendo-113-3-1025&partnerID=40&md5=4f79a38aa80deb11cc106188f6444b6f |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1210/endo-113-3-1025 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00137227_v113_n3_p1025_Tezon |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v113_n3_p1025_Tezon |y Registro en la Biblioteca Digital |
| 961 | |a paper_00137227_v113_n3_p1025_Tezon |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 79323 | ||