Properties and distribution of glucocorticoid-binding sites in cytosol of the spinal cord
We have examined the spinal cord for the presence of glucocorticoid-binding sites. For this purpose, cytosol from the spinal cord of adrenalectomized rats was incubated with (3H)-dexamethasone. Maximal binding was obtained after 20 h of incubation at 0°C in the presence of 20 mM molybdate, whereas a...
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| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1985
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| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04436caa a22006377a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18240 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204936.0 | ||
| 008 | 190411s1985 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0021981879 | |
| 024 | 7 | |2 cas |a 11beta,17beta dihydroxy 17 (1 propynyl)androsta 1,4,6 trien 3 one, 74915-58-5; aldosterone, 52-39-1, 6251-69-0; corticosterone, 50-22-6; dexamethasone, 50-02-2; estradiol, 50-28-2; progesterone, 57-83-0; testosterone, 58-22-0; triamcinolone acetonide, 76-25-5 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a NUNDA | ||
| 100 | 1 | |a Orti, E. | |
| 245 | 1 | 0 | |a Properties and distribution of glucocorticoid-binding sites in cytosol of the spinal cord |
| 260 | |c 1985 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a We have examined the spinal cord for the presence of glucocorticoid-binding sites. For this purpose, cytosol from the spinal cord of adrenalectomized rats was incubated with (3H)-dexamethasone. Maximal binding was obtained after 20 h of incubation at 0°C in the presence of 20 mM molybdate, whereas at 20° C the maximum was at 2 h. Using a range of (3H)-dexamethasone concentrations (0.2-30 nM), low capacity (161 ± 23 fmol/g protein) and high affinity (K(d) 3.2 ± 0.3 nM) sites were measured. Binding sites decreased by 25% and K(d) increased 2.5-fold after incubation with a pure glucocorticoid (RU 26988). Relative binding affinities of several competitors of 10 nM (3H)-dexamethasone were: triamcinolone acetonide 108, dexamethasone 100, RU 26988 54, corticosterone 18, progesterone 17, aldosterone 7, estradiol and testosterone < 1. Sedimentation coefficients in glycerol gradients containing molybdate were in the range of those published for glucocorticoid receptors (9.6-9.8 S). Binding of (3H)-dexamethasone was decreased by omitting a SH-protective agent from the buffer or by addition of SH-blocking reagents such as N-ethylmaleimide and p-chloromercuribenzoate. Using rats of different ages, it was found that binding sites were much lower in spinal cord from 2- to 8-day-old rats than in rats of 13-20 days and adults. Regional distribution studies using cytosol from spinal cords dissected between vertebrae C1-C2, C3-C7, T1-T8, T9-L3 and L4-L6 revealed that binding sites were higher in regions containing the cervical (C3-C7) and lumbar (T9-L3) enlargements, with respect to L4-L6. These studies suggest that the spinal cord contains cytosolic-binding sites resembling glucocorticoid receptors, and that the reported action of these steroids on spinal cord function could be mediated by these sites. |l eng | |
| 593 | |a Laboratorio de Esteroides, Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a 11BETA,17BETA DIHYDROXY 17 (1 PROPYNYL)ANDROSTA 1,4,6 TRIEN 3 ONE |
| 690 | 1 | 0 | |a ALDOSTERONE |
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a DEXAMETHASONE H 3 |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a GLUCOCORTICOID RECEPTOR |
| 690 | 1 | 0 | |a PROGESTERONE |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a TRIAMCINOLONE ACETONIDE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ADRENALECTOMY |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CENTRAL NERVOUS SYSTEM |
| 690 | 1 | 0 | |a DRUG DISTRIBUTION |
| 690 | 1 | 0 | |a DRUG EFFICACY |
| 690 | 1 | 0 | |a DRUG RECEPTOR BINDING |
| 690 | 1 | 0 | |a ENDOCRINE SYSTEM |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a ONTOGENY |
| 690 | 1 | 0 | |a PHARMACOKINETICS |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SPINAL CORD |
| 653 | 0 | 0 | |a ru 26988 |
| 700 | 1 | |a Coirini, H. | |
| 700 | 1 | |a De Nicola, A.F. | |
| 773 | 0 | |d 1985 |g v. 40 |h pp. 225-231 |k n. 3 |p NEUROENDOCRINOLOGY |x 00283835 |w (AR-BaUEN)CENRE-6253 |t Neuroendocrinology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021981879&doi=10.1159%2f000124134&partnerID=40&md5=6787c28dc9ee184a0522f2a5f4248290 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1159/000124134 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00283835_v40_n3_p225_Orti |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v40_n3_p225_Orti |y Registro en la Biblioteca Digital |
| 961 | |a paper_00283835_v40_n3_p225_Orti |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 79193 | ||