Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)

Chlorpyrifos (CPF) is a widely used organophosphate insecticide. The effect of chlorpyrifos exposure on acetylcholinesterase activity (AChE, biomarker of neurotoxicity) was evaluated in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). The animals were exposed to subleth...

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Autor principal: Bernal-Rey, D.L
Otros Autores: Dos Santos Afonso, M., Menendez-Helman, R.J
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Inderscience Enterprises Ltd. 2017
Acceso en línea:Registro en Scopus
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100 1 |a Bernal-Rey, D.L. 
245 1 0 |a Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis) 
260 |b Inderscience Enterprises Ltd.  |c 2017 
270 1 0 |m Menendez-Helman, R.J.; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Ciudad Universitaria Pabellón II 4to Piso, (C1428EHA)Argentina; email: rmenendez@qi.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a Chlorpyrifos (CPF) is a widely used organophosphate insecticide. The effect of chlorpyrifos exposure on acetylcholinesterase activity (AChE, biomarker of neurotoxicity) was evaluated in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). The animals were exposed to sublethal concentrations (1 and 5 μg CPF × L-1) or remained in control water for 96 h. AChE activity was inhibited 48% and 69% in C. decemmaculatus and 27% and 36% in G. affinis for 1 and 5 μg CPF × L-1, respectively. In vitro assays showed no differential sensitivity of the enzyme to CPF between species. However, the IC50 for in vitro exposure to chlorpyrifos-oxon (CPF-oxon, a product of the natural oxidative desulfuration of CPF) were 10.2 nM and 54.2 nM for C. decemmaculatus and G. affinis, respectively. Therefore, a different sensitivity of the enzyme between both species could explain the dissimilar behaviour of AChE after in vivo exposure to CPF. Copyright © 2017 Inderscience Enterprises Ltd.  |l eng 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: The authors gratefully acknowledge the Universidad de Buenos Aires and Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET) for financial support. 
593 |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Inorgánica, Analítica y Química Física, Ciudad Universitaria Pabellón II 3er Piso, (C1428EHA), Buenos Aires, Argentina 
593 |a CONICET-Universidad de Buenos Aires, Instituto de Química Física de Los Materiales, Medio Ambiente y Energía (INQUIMAE), Ciudad Universitaria Pabellón II 3er Piso, (C1428EHA), Buenos Aires, Argentina 
593 |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Ciudad Universitaria Pabellón II 4to Piso, (C1428EHA), Buenos Aires, Argentina 
593 |a CONICET-Universidad de Buenos Aires, Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Ciudad Universitaria Pabellón II 4to Piso, (C1428EHA), Buenos Aires, Argentina 
690 1 0 |a ACETYLCHOLINESTERASE 
690 1 0 |a ACHE 
690 1 0 |a BIOMARKERS 
690 1 0 |a CHLORPYRIFOS 
690 1 0 |a CNESTERODON DECEMMACULATUS 
690 1 0 |a FRESHWATER FISH 
690 1 0 |a GAMBUSIA AFFINIS 
690 1 0 |a PESTICIDES 
690 1 0 |a TELEOST. 
700 1 |a Dos Santos Afonso, M. 
700 1 |a Menendez-Helman, R.J. 
773 0 |d Inderscience Enterprises Ltd., 2017  |g v. 8  |h pp. 316-330  |k n. 4  |p Int. J. Environ. Health  |x 17434955  |t International Journal of Environment and Health 
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