Biosynthesis of paramylon in Euglena gracilis
Guardado en:
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1963
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 03752caa a22004337a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1748 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203105.0 | ||
| 008 | 190411s1963 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-4243783100 | |
| 024 | 7 | |2 cas |a Polysaccharides | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Goldemberg, S.H. | |
| 245 | 1 | 0 | |a Biosynthesis of paramylon in Euglena gracilis |
| 260 | |c 1963 | ||
| 270 | 1 | 0 | |m Goldemberg, S.H.; Instituto de Investigaciones Bioquímicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 536 | |a Detalles de la financiación: U.S. Public Health Service | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, T6cnicas | ||
| 536 | |a Detalles de la financiación: National Institutes of Health | ||
| 536 | |a Detalles de la financiación: Rockefeller Foundation | ||
| 536 | |a Detalles de la financiación: In order to identify the reaction product, the radioactive material was hydrolyzed with HC1 as indicated in Fig. I. It can be seen that the location of the radioactive spots corresponds with that of the oligosaccharides formed from similarly hydrolyzed laminarin, another/3-1,3-glucan, and also with that of authentic glucose, laminaribiose and laminaritriose. The radioactive product was also degraded enzymically using laminarase, a fl-I,3-glucan hydrolase obtained from E. gracilis 5. Radioactivity determinations showed that 519o of tile original counts were recovered in the supernatant after laminarase action. When chromatographed, these counts were located in the zone corresponding to glucose. Under the conditions of the experiment, laminarase hydrolyzed 4 ° % of the original paramylon* and only 2 % of starch, 1% of glycogen and 1% of soluble cellodextrin. The enzyme seems to quite specific for UDP-~-glucose. Thus adenosine diphosphate glucose was 25 % and deoxyadenosine diphosphate glucose 15 °o as effective as UDP-a-glucose, while with UDP-fl-glucose no activity could be detected. Further studies on the enzyme are being carried out. This investigation was supported in part by a research grant (No. G-3442) from tile National Institutes of Health, U.S. Public Health Service, by the Rockefeller Foundation and by the Consejo Nacional de Investigaciones Cientfficas y T6cnicas. Thanks are due to Dr. L. F. LELOIR for his continued interest and support and to all the members of the Instituto de Investigaciones Bioquimicas for their collaboration. | ||
| 593 | |a Instituto de Investigaciones Bioquímicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a POLYSACCHARIDE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a EUGLENA |
| 690 | 1 | 0 | |a EUGLENA |
| 690 | 1 | 0 | |a POLYSACCHARIDES |
| 690 | 1 | 0 | |a EUGLENA |
| 690 | 1 | 0 | |a POLYSACCHARIDES |
| 700 | 1 | |a Marechal, L.R. | |
| 773 | 0 | |d 1963 |g v. 71 |h pp. 743-744 |k n. C |x 00063002 |w (AR-BaUEN)CENRE-164 |t BBA - Biochimica et Biophysica Acta | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-4243783100&doi=10.1016%2f0006-3002%2863%2991155-7&partnerID=40&md5=8850a31ddfa83bcf4b3abeef869e2ba8 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0006-3002(63)91155-7 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00063002_v71_nC_p743_Goldemberg |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063002_v71_nC_p743_Goldemberg |y Registro en la Biblioteca Digital |
| 961 | |a paper_00063002_v71_nC_p743_Goldemberg |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 62701 | ||