Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the “Mar Menor” lagoon, in Murcia (Spain), and is the only “Critically Endangered” species of the genus Asparagus. Despite being protected, the number of plants has decreased in the las...
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Springer Netherlands
2018
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 10205caa a22009017a 4500 | ||
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| 001 | PAPER-17110 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204814.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85034084209 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PTCED | ||
| 100 | 1 | |a Regalado, J.J. | |
| 245 | 1 | 0 | |a Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk |
| 260 | |b Springer Netherlands |c 2018 | ||
| 270 | 1 | 0 | |m Regalado, J.J.; Laboratorio de Cultivo de Tejidos y Biotecnología, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, (CSIC-UMA)Spain; email: jjrg@eelm.csic.es |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Carmona-Martin, E., Regalado, J.J., Padilla, I.M.G., Westendorp, N., Encina, C.L., A new and efficient micropropagation method and its breeding applications in Asparagus genera (2014) Plant Cell Tiss Organ Cult, 119, pp. 479-488. , COI: 1:CAS:528:DC%2BC2cXht1aksrnL | ||
| 504 | |a Desjardins, Y., Micropropagation of Asparagus (Asparagus officinalis L.) (1992) Biotechnology in agriculture and forestry. vol 19: high-tech and micropropagation III, pp. 26-41. , Bajaj YPS, (ed), Springer, Berlin-Heidelberg | ||
| 504 | |a Desjardins, Y., Tiessen, H., Harney, P.M., The effect of sucrose and ancymidol on the in vitro rooting of nodal sections of asparagus (1987) HortScience, 22, pp. 131-133. , COI: 1:CAS:528:DyaL2sXhtleksLs%3D | ||
| 504 | |a Encina, C.L., Caro, E., Padilla, I.M.G., Westendorp, N., Carmona-Martín, E., Barceló-Muñoz, A., Vidoy Mercado, I., (2008) Procedimiento para la propagación in vitro del espárrago. Patent No.: P200803544 (ESP). Spain. Owner CSIC | ||
| 504 | |a Geoffriau, E., Denoue, D., Rameau, C., Assessment of genetic variation among asparagus (Asparagus officinalis L.) population and cultivars: agro-morphological and isoenzymatic data (1992) Euphytica, 61, pp. 169-179. , COI: 1:CAS:528:DyaK3sXpvVWqtw%3D%3D | ||
| 504 | |a (2017) The IUCN Red List of Threatened Species. Version 2017-2 | ||
| 504 | |a Knaflewski, M., Genealogy of asparagus cultivars (1996) Acta Hortic, 415, pp. 87-91 | ||
| 504 | |a Lallemand, J., Briand, F., Breuils, F., Denoue, D., Rameau, C., Identification of asparagus varieties by isozyme patterns (1994) Euphytica, 79, pp. 1-4. , COI: 1:CAS:528:DyaK2MXjvFSms78%3D | ||
| 504 | |a Moreno, R., Espejo, J.A., Cabrera, A., Millan, T., Gil, J., Ploidic and molecular analysis of ‘Morado de Huetor’ asparagus (Asparagus officinalis L.) population; a Spanish tetraploid landrace (2006) Genet Resour Crop Ev, 53, pp. 729-736. , COI: 1:CAS:528:DC%2BD28XmtFahsb4%3D | ||
| 504 | |a Moreno, R., Espejo, J.A., Cabrera, A., Gil, J., Origin of tetraploid cultivated asparagus landraces inferred from nuclear ribosomal DNA internal transcribed spacers polymorphisms (2008) Ann Appl Biol, 153, pp. 233-241. , COI: 1:CAS:528:DC%2BD1cXhtlWqsr7O | ||
| 504 | |a Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture (1962) Physiol Plantarum, 15, pp. 473-497. , COI: 1:CAS:528:DyaF3sXksFKm | ||
| 504 | |a Ochatt, S.J., Patat-Ochatt, E.M., Moessner, A., Ploidy level determination within the context of in vitro breeding (2011) Plant Cell Tiss Organ Cult, 104, pp. 329-341 | ||
| 504 | |a Pedrol, J., Regalado, J.J., López-Encina, C., Asparagus L.: Asparagus macrorrhizus (Pedrol, Regalado & López-Encina), sp. nov (2013) Flora Ibérica, 20, pp. 117-119. , Rico E, Crespo MB, Quintanar A, Herrero A, Aedo C, (eds), Real Jardín Botánico, CSIC, Madrid | ||
| 504 | |a Regalado, J.J., Carmona-Martín, E., Castro, P., Moreno, R., Gil, J., Encina, C.L., Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs (2015) Plant Cell Tiss Organ Cult, 121, pp. 501-510. , COI: 1:CAS:528:DC%2BC2MXhvVemt7o%3D | ||
| 504 | |a Regalado, J.J., Carmona-Martín, E., Castro, P., Moreno, R., Gil, J., Encina, C.L., Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L (2015) Plant Cell Tiss Organ Cult, 122, pp. 31-44. , COI: 1:CAS:528:DC%2BC2MXjsF2gs70%3D | ||
| 504 | |a Regalado, J.J., Moreno, R., Castro, P., Carmona-Martin, E., Rodríguez, R., Pedrol, J., Larrañaga, N., Encina, C.L., Asparagus macrorrhizus Pedrol, Regalado & López-Encina, an endemic species from Spain in extreme extinction risk, is a valuable genetic resource for asparagus breeding (2017) Genet Resour Crop Ev, 64, pp. 1581-1594. , COI: 1:CAS:528:DC%2BC28Xhs1GqsLzJ | ||
| 504 | |a Sánchez-Gómez, P., Carrión, M.A., Hernández, A., Guerra, J., (2002) Libro Rojo de la Flora Silvestre Protegida de la Región de Murcia. Dirección General del Medio Natural. Consejería de Agricultura, Agua y Medio Ambiente. Murcia | ||
| 504 | |a Sánchez-Gómez, P., Vera-Pérez, J.B., Jiménez-Martínez, J.F., Aedo, C., Pedrol, J., La esparraguera marina, especie en peligro crítico de extinción en la península ibérica (2007) Conservación Vegetal, 11, pp. 13-14 | ||
| 504 | |a Sánchez-Gómez, P., Vera-Pérez, J.B., Jiménez Martínez, J.F., (2008) Libro Rojo de la Flora Silvestre Protegida de la Región de Murcia. Dirección General del Medio Natural. Consejería de Agricultura, Agua y Medio Ambiente. Murcia | ||
| 520 | 3 | |a Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the “Mar Menor” lagoon, in Murcia (Spain), and is the only “Critically Endangered” species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l− 1 NAA, 0.1 mg l− 1 KIN, 2 mg l− 1 ancymidol and 6% sucrose. A percentage of 69.7 ± 8.0% of the rhizome buds developed shoots, but only 17.4 ± 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 ± 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l− 1 IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species. © 2017, Springer Science+Business Media B.V., part of Springer Nature. |l eng | |
| 536 | |a Detalles de la financiación: Junta de Andalucía, AGR3648 | ||
| 536 | |a Detalles de la financiación: Acknowledgements Authors would like to thank to the Official Nursery of the Directorate General for the Environment belonging to the Council of Agriculture and Water of the Region of Murcia (Spain) for facilitating the plants of A. macrorrhizus used in this work. This study was funded by “Junta de Andalucía” (Project of Excellence AGR3648). INMIBO Publication No. 216. Doctor Regalado Gonzalez is a PostDoctoral Fellow at CONICET. | ||
| 593 | |a Laboratorio de Cultivo de Tejidos y Biotecnología, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, (CSIC-UMA), Algarrobo-Costa, Málaga 29750, Spain | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Cultivo Experimental de Plantas y Microalgas, No. 68, Ciudad Autónoma de Buenos Aires, Argentina | ||
| 593 | |a CONICET - Universidad de Buenos Aires, Instituto de Micología y Botánica (INMIBO), Ciudad Autónoma de Buenos Aires, Argentina | ||
| 593 | |a Dpto. Genética, ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edif. C-5, Córdoba, 14071, Spain | ||
| 593 | |a Dpto. Fitoquímica de Alimentos. Instituto de la Grasa (CSIC) – Universidad Pablo de Olavide, Edificio 46. Ctra. de Utrera, km. 1, Sevilla, 41013, Spain | ||
| 690 | 1 | 0 | |a ASPARAGUS SPP |
| 690 | 1 | 0 | |a ENDANGERED SPECIES |
| 690 | 1 | 0 | |a FLOW CYTOMETRY |
| 690 | 1 | 0 | |a GERMPLASM PRESERVATION |
| 690 | 1 | 0 | |a RHIZOME BUDS |
| 690 | 1 | 0 | |a CONSERVATION |
| 690 | 1 | 0 | |a FLOW CYTOMETRY |
| 690 | 1 | 0 | |a SUGAR (SUCROSE) |
| 690 | 1 | 0 | |a ASPARAGUS SPP |
| 690 | 1 | 0 | |a CRITICALLY ENDANGERED |
| 690 | 1 | 0 | |a ENDANGERED SPECIES |
| 690 | 1 | 0 | |a EXTINCTION RISKS |
| 690 | 1 | 0 | |a GENETIC RESOURCES |
| 690 | 1 | 0 | |a GERMPLASMS |
| 690 | 1 | 0 | |a MICROPROPAGATED PLANTLETS |
| 690 | 1 | 0 | |a RHIZOME BUDS |
| 690 | 1 | 0 | |a PLANTS (BOTANY) |
| 690 | 1 | 0 | |a BIODIVERSITY |
| 690 | 1 | 0 | |a GENETIC ENGINEERING |
| 690 | 1 | 0 | |a PLANTS |
| 690 | 1 | 0 | |a RISK ASSESSMENT |
| 700 | 1 | |a Carmona-Martin, E. | |
| 700 | 1 | |a López-Granero, M. | |
| 700 | 1 | |a Jiménez-Araujo, A. | |
| 700 | 1 | |a Castro, P. | |
| 700 | 1 | |a Encina, C.L. | |
| 773 | 0 | |d Springer Netherlands, 2018 |g v. 132 |h pp. 573-578 |k n. 3 |p Plant Cell Tissue Organ Cult. |x 01676857 |t Plant Cell, Tissue and Organ Culture | |
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