Drp-1 dependent mitochondrial fragmentation and protective autophagy in dopaminergic SH-SY5Y cells overexpressing alpha-synuclein
Parkinson's disease is a neurodegenerative movement disorder caused by the loss of dopaminergic neurons from substantia nigra. It is characterized by the accumulation of aggregated α-synuclein as the major component of the Lewy bodies. Additional common features of this disease are the mitochon...
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Academic Press Inc.
2018
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| LEADER | 21339caa a22019577a 4500 | ||
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| 001 | PAPER-17015 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204807.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85041563990 | |
| 024 | 7 | |2 cas |a alpha synuclein, 154040-18-3; guanosine triphosphatase, 9059-32-9; alpha-Synuclein; DNM1L protein, human; GTP Phosphohydrolases; Microtubule-Associated Proteins; Mitochondrial Proteins; OPA1 protein, human; SNCA protein, human | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MOCNE | ||
| 100 | 1 | |a Martinez, J.H. | |
| 245 | 1 | 0 | |a Drp-1 dependent mitochondrial fragmentation and protective autophagy in dopaminergic SH-SY5Y cells overexpressing alpha-synuclein |
| 260 | |b Academic Press Inc. |c 2018 | ||
| 270 | 1 | 0 | |m Kotler, M.L.; Ciudad Universitaria, Avda. Intendente Güiraldes 2160, Argentina; email: kotler@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Parkinson's disease is a neurodegenerative movement disorder caused by the loss of dopaminergic neurons from substantia nigra. It is characterized by the accumulation of aggregated α-synuclein as the major component of the Lewy bodies. Additional common features of this disease are the mitochondrial dysfunction and the activation/inhibition of autophagy both events associated to the intracellular accumulation of α-synuclein. The mechanism by which these events contribute to neural degeneration remains unknown. In the present work we investigated the effect of α-synuclein on mitochondrial dynamics and autophagy/mitophagy in SH-SY5Y cells, an in vitro model of Parkinson disease. We demonstrated that overexpression of wild type α-synuclein causes moderated toxicity, ROS generation and mitochondrial dysfunction. In addition, α-synuclein induces the mitochondrial fragmentation on a Drp-1-dependent fashion. Overexpression of the fusion protein Opa-1 prevented both mitochondrial fragmentation and cytotoxicity. On the other hand, cells expressing α-synuclein showed activated autophagy and particularly mitophagy. Employing a genetic strategy we demonstrated that autophagy is triggered in order to protect cells from α-synuclein-induced cell death. Our results clarify the role of Opa-1 and Drp-1 in mitochondrial dynamics and cell survival, a controversial α-synuclein research issue. The findings presented point to the relevance of mitochondrial homeostasis and autophagy in the pathogenesis of PD. Better understanding of the molecular interaction between these processes could give rise to novel therapeutic methods for PD prevention and amelioration. © 2018 Elsevier Inc. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET | ||
| 536 | |a Detalles de la financiación: UBACyT 2014, 0573, 20020130200025BA, PIP No. 0356, 0519, 0771 | ||
| 536 | |a Detalles de la financiación: This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas ( CONICET PIP No. 0356 , No. 0771 , No. 0519 and No.0573 ) and UBACyT 2014–2017 No. 20020130200025BA . We thank Dr. Tom Jovin (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) for providing relevant materials. JHM, RMG and SPA are supported by CONICET scholarships. FF is a member of CPA at CONICET. AA, FCL and MLK are researchers at CONICET. | ||
| 593 | |a CONICET- Universidad de Buenos Aires, Instituto de Química Biológica Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio Interdisciplinario de Dinámica Celular y Nanoherramientas, Argentina | ||
| 593 | |a CONICET- Universidad de Buenos Aires, Instituto de Química Biológica Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Disfunción Celular en Enfermedades Neurodegenerativas y Nanomedicina, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Medicina Experimental (IMEX)-CONICET, Academia Nacional de Medicina, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ALPHA-SYNUCLEIN |
| 690 | 1 | 0 | |a AUTOPHAGY |
| 690 | 1 | 0 | |a MITOCHONDRIA |
| 690 | 1 | 0 | |a MITOCHONDRIAL DYNAMICS |
| 690 | 1 | 0 | |a MITOPHAGY |
| 690 | 1 | 0 | |a PARKINSON'S DISEASE |
| 690 | 1 | 0 | |a ALPHA SYNUCLEIN |
| 690 | 1 | 0 | |a DYNAMIN RELATED PROTEIN 1 |
| 690 | 1 | 0 | |a HYBRID PROTEIN |
| 690 | 1 | 0 | |a MITOCHONDRIAL PROTEIN |
| 690 | 1 | 0 | |a OPTIC ATROPHY 1 PROTEIN |
| 690 | 1 | 0 | |a REACTIVE OXYGEN METABOLITE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ALPHA SYNUCLEIN |
| 690 | 1 | 0 | |a DNM1L PROTEIN, HUMAN |
| 690 | 1 | 0 | |a GUANOSINE TRIPHOSPHATASE |
| 690 | 1 | 0 | |a MICROTUBULE ASSOCIATED PROTEIN |
| 690 | 1 | 0 | |a MITOCHONDRIAL PROTEIN |
| 690 | 1 | 0 | |a OPA1 PROTEIN, HUMAN |
| 690 | 1 | 0 | |a SNCA PROTEIN, HUMAN |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a AUTOPHAGY |
| 690 | 1 | 0 | |a CELL DEATH |
| 690 | 1 | 0 | |a CELL PROTECTION |
| 690 | 1 | 0 | |a CELL SURVIVAL |
| 690 | 1 | 0 | |a CELL VIABILITY |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a CYTOTOXICITY |
| 690 | 1 | 0 | |a DISORDERS OF MITOCHONDRIAL FUNCTIONS |
| 690 | 1 | 0 | |a DOPAMINERGIC NERVE CELL |
| 690 | 1 | 0 | |a GENE OVEREXPRESSION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a MITOCHONDRIAL DYNAMICS |
| 690 | 1 | 0 | |a MITOCHONDRIAL FRAGMENTATION |
| 690 | 1 | 0 | |a MITOPHAGY |
| 690 | 1 | 0 | |a PARKINSON DISEASE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a SH-SY5Y CELL LINE |
| 690 | 1 | 0 | |a WILD TYPE |
| 690 | 1 | 0 | |a AUTOPHAGY |
| 690 | 1 | 0 | |a DOPAMINERGIC NERVE CELL |
| 690 | 1 | 0 | |a GENETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MITOCHONDRION |
| 690 | 1 | 0 | |a PHYSIOLOGY |
| 690 | 1 | 0 | |a SUBSTANTIA NIGRA |
| 690 | 1 | 0 | |a TUMOR CELL LINE |
| 690 | 1 | 0 | |a ALPHA-SYNUCLEIN |
| 690 | 1 | 0 | |a AUTOPHAGY |
| 690 | 1 | 0 | |a CELL LINE, TUMOR |
| 690 | 1 | 0 | |a DOPAMINERGIC NEURONS |
| 690 | 1 | 0 | |a GTP PHOSPHOHYDROLASES |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a MICROTUBULE-ASSOCIATED PROTEINS |
| 690 | 1 | 0 | |a MITOCHONDRIA |
| 690 | 1 | 0 | |a MITOCHONDRIAL DEGRADATION |
| 690 | 1 | 0 | |a MITOCHONDRIAL DYNAMICS |
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| 690 | 1 | 0 | |a PARKINSON DISEASE |
| 690 | 1 | 0 | |a SUBSTANTIA NIGRA |
| 700 | 1 | |a Alaimo, A. | |
| 700 | 1 | |a Gorojod, R.M. | |
| 700 | 1 | |a Porte Alcon, S. | |
| 700 | 1 | |a Fuentes, F. | |
| 700 | 1 | |a Coluccio Leskow, F. | |
| 700 | 1 | |a Kotler, M.L. | |
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