Further studies on high molecular weight liver glycogen

A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a n...

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Detalles Bibliográficos
Autor principal: Mordoh, J.
Otros Autores: Krisman, C.R, Leloir, L.F
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1966
Acceso en línea:Registro en Scopus
DOI
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0013879262 
024 7 |2 cas  |a sodium hydroxide, 1310-73-2; urea, 57-13-6; Guanidines; Liver Glycogen; Oligosaccharides; Sodium Hydroxide, 1310-73-2; Urea, 57-13-6 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a ABBIA 
100 1 |a Mordoh, J. 
245 1 0 |a Further studies on high molecular weight liver glycogen 
260 |c 1966 
270 1 0 |m Mordoh, J.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facuĺtad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina 
506 |2 openaire  |e Política editorial 
520 3 |a A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a new method and was found to remain constant under conditions in which glycogen content varied considerably. Native glycogen was compared with that prepared in vitro with purified enzymes. Although both preparations were similar in molecular weight and in their appearance under the electron microscope, they differed in the type of breakdown produced by acid, alkali, heat, and ultrasonic vibrations. When glycogen prepared in vitro was submitted to these procedures, its sedimentation coefficient decreased progressively, while native glycogen was broken down preferentially to a lighter population of about S = 100. © 1966.  |l eng 
536 |a Detalles de la financiación: U.S. Public Health Service 
536 |a Detalles de la financiación: National Institutes of Health 
536 |a Detalles de la financiación: Rockefeller Foundation 
536 |a Detalles de la financiación: 1 This investigation was supported in part by a research grant (GM 03442) from the National Institutes of Health, U.S. Public Health Service; by the Rockefeller Foundation; and by the Con-sejo National de Investigaciones Cientificas y T6cnicas (Repdblica Argentina). 2 Fellow of the Consejo National de Investi-gaciones Cientificas y Tecnicas (Repdblica Argentina). 
593 |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facuĺtad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina 
690 1 0 |a GUANIDINE DERIVATIVE 
690 1 0 |a OLIGOSACCHARIDE 
690 1 0 |a SODIUM HYDROXIDE 
690 1 0 |a UREA 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a CELL FRACTIONATION 
690 1 0 |a CHEMISTRY 
690 1 0 |a GLYCOGEN LIVER LEVEL 
690 1 0 |a HEAT 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a RAT 
690 1 0 |a ULTRASOUND 
690 1 0 |a ANIMAL 
690 1 0 |a CHEMISTRY 
690 1 0 |a GUANIDINES 
690 1 0 |a HEAT 
690 1 0 |a IN VITRO 
690 1 0 |a LIVER GLYCOGEN 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a OLIGOSACCHARIDES 
690 1 0 |a RATS 
690 1 0 |a SODIUM HYDROXIDE 
690 1 0 |a SUBCELLULAR FRACTIONS 
690 1 0 |a ULTRASONICS 
690 1 0 |a UREA 
700 1 |a Krisman, C.R. 
700 1 |a Leloir, L.F. 
773 0 |d 1966  |g v. 113  |h pp. 265-272  |k n. 2  |p Arch. Biochem. Biophys.  |x 00039861  |w (AR-BaUEN)CENRE-1377  |t Archives of Biochemistry and Biophysics 
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