Alpha-synuclein mitochondrial interaction leads to irreversible translocation and complex I impairment
α-synuclein is involved in both familial and sporadic Parkinson's disease. Although its interaction with mitochondria has been well documented, several aspects remains unknown or under debate such as the specific sub-mitochondrial localization or the dynamics of the interaction. It has been sug...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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Academic Press Inc.
2018
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| LEADER | 21646caa a22017177a 4500 | ||
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| 001 | PAPER-16841 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204753.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85047244932 | |
| 024 | 7 | |2 cas |a adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; alpha synuclein, 154040-18-3; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone), 9028-04-0 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ABBIA | ||
| 100 | 1 | |a Martínez, J.H. | |
| 245 | 1 | 0 | |a Alpha-synuclein mitochondrial interaction leads to irreversible translocation and complex I impairment |
| 260 | |b Academic Press Inc. |c 2018 | ||
| 270 | 1 | 0 | |m Velazquez, F.; CONICET, Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)Argentina; email: fvelazquez@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Vanasco, V., Magnani, N.D., Cimolai, M.C., Valdez, L.B., Evelson, P., Boveris, A., Alvarez, S., Endotoxemia impairs heart mitochondrial function by decreasing electron transfer, ATP synthesis and ATP content without affecting membrane potential (2012) J. Bioenerg. Biomembr., 44, pp. 243-252 | ||
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| 520 | 3 | |a α-synuclein is involved in both familial and sporadic Parkinson's disease. Although its interaction with mitochondria has been well documented, several aspects remains unknown or under debate such as the specific sub-mitochondrial localization or the dynamics of the interaction. It has been suggested that α-synuclein could only interact with ER-associated mitochondria. The vast use of model systems and experimental conditions makes difficult to compare results and extract definitive conclusions. Here we tackle this by analyzing, in a simplified system, the interaction between purified α-synuclein and isolated rat brain mitochondria. This work shows that wild type α-synuclein interacts with isolated mitochondria and translocates into the mitochondrial matrix. This interaction and the irreversibility of α-synuclein translocation depend on incubation time and α-synuclein concentration. FRET experiments show that α-synuclein localizes close to components of the TOM complex suggesting a passive transport of α-synuclein through the outer membrane. In addition, α-synuclein binding alters mitochondrial function at the level of Complex I leading to a decrease in ATP synthesis and an increase of ROS production. © 2018 |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, PIP 11220110100573 | ||
| 536 | |a Detalles de la financiación: To the memory of Dr. Elizabeth Jares-Erijman, who was supervisor of JHM and first envisioned this project. We acknowledge Professor Thomas Jovin for financial support, and supervision of the work at early stages. This research was supported by Projects UBACyT 20020100100465BA and UBACyT 20020130200025BA from Universidad de Buenos Aires , and PIP 11220110100573 from CONICET of Argentina. Appendix A | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina | ||
| 593 | |a CONICET, Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina | ||
| 593 | |a Instituto de Medicina Experimental- IMEX, CONICET, Academia Nacional de Medicina, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Bioquímica y Medicina Molecular (IBIMOL), Universidad de Buenos Aires, Argentina | ||
| 593 | |a CEINBIO, Depto. Bioquímica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a MITOCHONDRIA |
| 690 | 1 | 0 | |a MITOCHONDRIAL METABOLISM |
| 690 | 1 | 0 | |a PARKINSON'S DISEASE |
| 690 | 1 | 0 | |a Α-SYNUCLEIN |
| 690 | 1 | 0 | |a ADENOSINE TRIPHOSPHATE |
| 690 | 1 | 0 | |a ALPHA SYNUCLEIN |
| 690 | 1 | 0 | |a REACTIVE OXYGEN METABOLITE |
| 690 | 1 | 0 | |a RECOMBINANT PROTEIN |
| 690 | 1 | 0 | |a REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE) |
| 690 | 1 | 0 | |a TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20 |
| 690 | 1 | 0 | |a ADULT |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BRAIN MITOCHONDRION |
| 690 | 1 | 0 | |a CELL ISOLATION |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ELECTRON TRANSPORT |
| 690 | 1 | 0 | |a FLUORESCENCE RESONANCE ENERGY TRANSFER |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MITOCHONDRIAL MEMBRANE POTENTIAL |
| 690 | 1 | 0 | |a MITOCHONDRIAL RESPIRATION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a NUCLEIC ACID SYNTHESIS |
| 690 | 1 | 0 | |a OUTER MEMBRANE |
| 690 | 1 | 0 | |a PASSIVE TRANSPORT |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN INTERACTION |
| 690 | 1 | 0 | |a PROTEIN LOCALIZATION |
| 690 | 1 | 0 | |a PROTEIN PURIFICATION |
| 690 | 1 | 0 | |a PROTEIN TRANSPORT |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a RESPIRATORY CHAIN |
| 700 | 1 | |a Fuentes, F. | |
| 700 | 1 | |a Vanasco, V. | |
| 700 | 1 | |a Alvarez, S. | |
| 700 | 1 | |a Alaimo, A. | |
| 700 | 1 | |a Cassina, A. | |
| 700 | 1 | |a Coluccio Leskow, F. | |
| 700 | 1 | |a Velazquez, F. | |
| 773 | 0 | |d Academic Press Inc., 2018 |g v. 651 |h pp. 1-12 |p Arch. Biochem. Biophys. |x 00039861 |w (AR-BaUEN)CENRE-1377 |t Archives of Biochemistry and Biophysics | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00039861_v651_n_p1_Martinez |y Handle |
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