Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red

In this work we describe the formation and microscopical application of a fluorescentderivative of Ruthenium Red (RR) obtained by heating the dye in the presence of 1,10-phenanthroline (OP). The RR-OP reaction product showed absorption maxima at 416 and 444 nm and intense fluorescence emission at 57...

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Autor principal: Bertolesi, G.E
Otros Autores: de Cidre, L.L, Stockert, J.C
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1995
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0028868641 
024 7 |2 cas  |a ruthenium red, 11103-72-3, 1307-52-4 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Bertolesi, G.E. 
245 1 0 |a Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red 
260 |c 1995 
270 1 0 |m Bertolesi, G.E.; Cátedra de Histologia Animal, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, y Area de Investigación, Instituto de Oncologia “A. H. Roffo”, Universidad de Buenos AiresArgentina 
506 |2 openaire  |e Política editorial 
504 |a Barton, JK, Tris(phenanthroline)metal complexes: probes for DNA helicity (1983) J Biomol Struct Dyn, 1, pp. 621-632 
504 |a Barton, JK, Danishefsky, AT, Goldberg, JM, Tris (phenanthroline) ruthenium(II): stereoselectivity in binding to DNA (1984) J Am Chem Soc, 106, pp. 2172-2176 
504 |a Barton, JK, Goldberg, JM, Kumar, CV, Turro, NJ, Binding modes and base specificity of tris(phenanthroline)ruthenium(II) enantiomers with nucleic acids: tuning the stereoselectivity (1986) J Am Chem Soc, 108, pp. 2081-2088 
504 |a Bertolesi, GE, Trigoso, CI, Espada, J, Stockert, JC, Cytochemical application of tris (2,2/-bipyridine) ruthenium (II): fluorescence reaction with sulfated polyanions of mast cell granules (1995) J Histochem Cytochem, 43, pp. 537-543 
504 |a Bradley, PG, Kress, N, Hornberger, BA, Dallinger, RF, Woodruff, WH, Vibrational spectroscopy of the electronically excited state. 5. Time-resolved resonance Raman study of tris(bipyridine)ruthenium(II)and related complexes. Definitive evidence for the “localized” MLCT state (1981) J Am Chem Soc, 103, pp. 7441-7446 
504 |a Carrondo, MAAF, Griffith, WP, Hall, JP, Skapsky, AC, X-ray structure of [Ru3O2(NH3)14]6+ cation of the cytological reagent ruthenium red (1980) Biochim Biophys Acta, 627, pp. 332-334 
504 |a Cowden, RE, Curtis, SK, Microfluorimetric investigations of chromatin structure. II. Mordant fluorochroming with ions that complex with morin (1981) Histochemistry, 72, pp. 391-400 
504 |a Del Castillo, P, Llorente, AR, Gomez, A, Gosálvez, J, Goyanes, VJ, Stockert, JC, New fluorescence reactions in DNA cytochemistry. 2. Microscopic and spectroscopic studies on fluorescent aluminum complexes (1990) Analyt Quant Cytol Histol, 12, pp. 11-20 
504 |a Fletcher, JM, Greenfield, BF, Hardy, CJ, Scargill, D, Woodhead, JL, Ruthenium red (1961) Journal of the Chemical Society (Resumed), pp. 2000-2006 
504 |a Friedman, AE, Kumar, CV, Turro, NJ, Barton, JK, Luminescence of ruthenium(II) polypyridyls:evidence for intercalative binding to Z-DNA (1991) Nucleic Acids Res, 19, pp. 2595-2602 
504 |a Gustafson, GT, Pihl, E, Staining of mast cell acid glycosaminoglycans in ultrathin sections by ruthenium red (1967) Nature, 216, pp. 697-698 
504 |a Gutiérrez-Gonzálvez, MG, Stockert, JC, Ferrer, JM, Tato, A, Ruthenium red staining of polyanion containing structures in sections from epoxy-resin embedded tissues (1984) Acta histochem, 74, pp. 115-120 
504 |a Gutiérrez-Gonzálvez, MG, Armas-Portela, R, Stockert, JC, Differential staining of biological structures by ruthenium red (1987) J Microscopy, 145, pp. 333-340 
504 |a Hanke, DE, Northcote, DH, Molecular visualization of pectin and DNA by ruthenium red (1975) Biopolymers, 14, pp. 1-17 
504 |a Hayat, MA, (1975) Positive staining for electron microscopy, , Van Nostrand Reinhold, Basel 
504 |a Jenkins, Y, Friedman, AE, Turro, NJ, Barton, JK, Characterization of dipyridophenazine complexes of ruthenium(II): the light switch effect as a function of nucleic acid sequence and conformation (1992) Biochemistry, 31, pp. 10809-10816 
504 |a Jorgensen, CK, Orgel, LE, Energy levels and π bonding in polynuclear complexes (1961) Molecular Physics, 4, pp. 215-218 
504 |a Karpel, RL, Shirley, MS, Holt, SR, Interaction of the ruthenium red cation with nucleic acid double helices (1981) Biophys Chem, 13, pp. 151-165 
504 |a Kelly, JM, Tossi, AB, McConnell, DJ, OhUigin, C, A study of the interactions of some polypyridylruthenium(II) complexes with DNA using fluorescence spectroscopy, topoisomerisation and thermal denaturation (1985) Nucleic Acids Res, 13, pp. 6017-6034 
504 |a Lagunoff, D, Vital staining of mast cells with ruthenium red (1972) J Histochem Cytochem, 20, pp. 938-944 
504 |a Lillie, RD, (1977) H. J. Conn's Biological Stains, , 7th ed, Williams & Wilkins, New York 
504 |a Llorente, AR, Del Castillo, P, Stockert, JC, Aluminium binding to chromatin DNA as revealed by formation of fluorescent complexes with 8-hydroxyquinoline and other ligands (1989) Journal of Microscopy, 155, pp. 227-230 
504 |a Luft, JH, Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action (1971) Anat Rec, 171, pp. 347-368 
504 |a McWhinnie, WR, Miller, JD, The chemistry of complexes containing 2,2' -bipyridyl, 1,10-phenanthroline, or 2,2,,6,2//-terpyridyl as ligands (1969) Adv Inorg Chem Radiochem, 12, pp. 135-215 
504 |a Myers, DB, Highton, TC, Rayns, DG, Ruthenium red-positive filaments interconnecting collagen fibrils (1973) J Ultrastruct Res, 42, pp. 87-92 
504 |a Rehmann, JP, Barton, JK, 1H NMR studies of tris(phenanthroline) metal complexes bound to oligonucleotides: characterization of binding modes (1990) Biochemistry, 29, pp. 1701-1709 
504 |a Robin, MB, Day, P, Mixed valence chemistry: a survey and classification (1967) Adv Inorg Chem Radiochem, 10, pp. 247-422 
504 |a Ruggeri, A, Dell'Orbo, C, Quacci, D, Electron microscopic visualization of proteoglycans with ruthenium red (1977) Histochem J, 9, pp. 249-252 
504 |a Schilt, AA, Analytical applications of 1,10-phenanthroline and related compounds (1969) International Series of Monographs in Analytical Chemistry, , Vol 32, Pergamon Press, Baltimore 
504 |a Smith, PM, Fealey, T, Earley, JE, Silverton, JV, The crystal and molecular structure of di-μ-oxo-bis(pentaammineruthenium)bis(ethylenediamine)ruthenium hexachloride. The ethylenediamine analog of“rutheni (1971) Inorg Chem, 10, pp. 1943-1947 
504 |a Stockert, JC, Paniagua, R, Ruthenium red staining of chromatin in Epon sections (1980) Z Naturforsch, 35, pp. 348-349 
504 |a Stockert, JC, Pelling, C, DNA cytochemistry in polytene chromosomes: Electron contrasting agents for the ultrastructural detection of chromatin DNA after alkaline hydrolysis/methylatio (1992) Acta histochem, 93, pp. 298-306 
520 3 |a In this work we describe the formation and microscopical application of a fluorescentderivative of Ruthenium Red (RR) obtained by heating the dye in the presence of 1,10-phenanthroline (OP). The RR-OP reaction product showed absorption maxima at 416 and 444 nm and intense fluorescence emission at 578 nm under 440 nm exciting light. Neither RR nor OP solutions alone were fluorescent when excited at 440 nm. Using fluorescence microscopy, chicken blood cell smears stained 5min with the RR-OP derivative showed the chromatin of erythrocyte nuclei with a bright orange fluorescence under violet-blue (436 nm) exciting light. © 1995, Gustav Fisher Verlag Jena GmbH. All rights reserved.  |l eng 
536 |a Detalles de la financiación: PM91-0021 
536 |a Detalles de la financiación: Universidad de Buenos Aires, EX199 
536 |a Detalles de la financiación: Consejo Superior de Investigaciones Científicas 
536 |a Detalles de la financiación: We are greatly indebted to Dr C. I. Trigoso for her valuable collaboration. This work was supported by grants from the Universidad de Buenos Aires (EX199) Argentina, Direccion General de Investigacion Cientifica y Tecnica (PM91-0021) Spain, and the Cooperation Program between Max-Planck-Gesellschaft (Federal Republic of Germany) and Consejo Superior de Investigaciones Cientificas (Spain). 
593 |a Cátedra de Histologia Animal, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, y Area de Investigación, Instituto de Oncologia “A. H. Roffo”, Universidad de Buenos Aires, Argentina 
593 |a Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantobianco, Madrid, E-28049, Spain 
593 |a Max-Planck-Institut für Biologie, Abteilung Mikrobiologie, Tübingen, D-72076, Germany 
690 1 0 |a 1,10-PHENANTHROLINE 
690 1 0 |a CHROMATIN FLUORESCENCE 
690 1 0 |a FLUORESCENCE MICROSCOPY 
690 1 0 |a FLUORESCENT RUTHENIUM COMPLEXES 
690 1 0 |a RUTHENIUM RED 
690 1 0 |a 1,10 PHENANTHROLINE DERIVATIVE 
690 1 0 |a RUTHENIUM RED 
690 1 0 |a RUTHENIUM RED DERIVATIVE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ARTICLE 
690 1 0 |a CHICKEN 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a ERYTHROCYTE 
690 1 0 |a FLUORESCENCE MICROSCOPY 
690 1 0 |a NONHUMAN 
690 1 0 |a TECHNIQUE 
690 1 0 |a ANIMALIA 
690 1 0 |a GALLUS GALLUS 
690 1 0 |a VIOLA (ANGIOSPERM) 
700 1 |a de Cidre, L.L. 
700 1 |a Stockert, J.C. 
773 0 |d 1995  |g v. 97  |h pp. 401-408  |k n. 4  |p Acta Histochem.  |x 00651281  |t Acta Histochemica 
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