Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems
Polypeptide synthesis programmed by poly(U) and globin mRNA has been studied in cell-free extracts from wheat germ. A two-step reaction with a preincubation at high Mg++ levels followed by a second step carried out after a shift to a low Mg++ concentration and the addition of labeled amino acids is...
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| Autor principal: | |
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1980
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 05044caa a22007817a 4500 | ||
|---|---|---|---|
| 001 | PAPER-16512 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204732.0 | ||
| 008 | 190411s1980 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85025574750 | |
| 024 | 7 | |2 cas |a aurintricarboxylic acid, 4431-00-9; magnesium, 7439-95-4; polyuridylic acid, 27416-86-0; Aurintricarboxylic Acid, 4431-00-9; Globins, 9004-22-2; Magnesium, 7439-95-4; Poly U, 27416-86-0, 9004-22-2; RNA, Messenger | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Algranati, I.D. | |
| 245 | 1 | 0 | |a Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems |
| 260 | |c 1980 | ||
| 270 | 1 | 0 | |m Algranati, I.D.; Instituto de Investigaciones Bioquímicas “Fundación Campomar” Obligado 2490, Buenos Aires, 1428, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Schreier, M.H., Noll, H., (1971) Proc. Natl. Acad. Sci. U. S. A, 68, pp. 805-809. , second ed | ||
| 504 | |a Haselkorn, R., Rothman-Denes, L.F., (1973) Ann. Rev. Biochem, 42, pp. 397-438 | ||
| 504 | |a Shafritz, D.A., Prichard, P.M., Gilbert, J.M., Anderson, W.F., (1970) Biochem. Biophys. Res. Commun, 38, pp. 721-727 | ||
| 504 | |a Marcus, A., Efron, D., Weeks, D.P., (1974) Methods in Enzymology, 30, pp. 749-754. , K. Moldave, L. Grossman, second ed | ||
| 504 | |a Efron, D., Marcus, A., (1973) FEBS Lett, 33, pp. 23-27 | ||
| 504 | |a Revel, M., (1977) Molecular Mechanisms of Protein Biosynthesis, pp. 245-321. , H. Weissbach, S. Pestka, Academic Press, New York | ||
| 504 | |a Weissbach, H., Ochoa, S., (1976) Ann. Rev. Biochem, 45, pp. 191-216 | ||
| 504 | |a Huang, M.T., Grollman, A.P., (1972) Mol. Pharmacol, 8, pp. 111-127 | ||
| 504 | |a Marcus, A., Bewley, J.D., Weeks, D.P., (1970) Science, 167, pp. 1735-1736 | ||
| 504 | |a Vázquez, D., (1979) Inhibitors of Protein Biosynthesis, pp. 28-30. , Springer Verlag, Berlin | ||
| 504 | |a Lodish, H.F., Housman, D., Jacobsen, M., (1971) Biochemistry, 10, pp. 2348-2356 | ||
| 504 | |a Culp, W.J., McKeehan, W.L., Hardesty, B., (1969) Proc. Natl. Acad. Sci. U. S. A, 63, pp. 1431-1438. , second ed | ||
| 520 | 3 | |a Polypeptide synthesis programmed by poly(U) and globin mRNA has been studied in cell-free extracts from wheat germ. A two-step reaction with a preincubation at high Mg++ levels followed by a second step carried out after a shift to a low Mg++ concentration and the addition of labeled amino acids is described. Under these conditions the initiation of polyphenylalanine synthesis can be blocked without affecting the elongation of polypeptide chains. This procedure allows the selective inhibition of polypeptide synthesis initiation without using any drug or antibiotic. © 1980, All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Acknowledgements - I am grateful to Drs. L. F. Leloir, N. S. Gonzalez, S. H. Goldemberg, M. Dankert, M. Garcfa-Patrone and all members of the Instituto de Investigaciones Bioquimicas for many helpful discussions. I thank Mrs. Marcela Ferrer for the preparation of the wheat germ cell-free extracts. I. D. A. is a Career Investigator of the Consejo National de Investigaciones Cientificas y Tdcnicas (Argentina). | ||
| 593 | |a Instituto de Investigaciones Bioquímicas “Fundación Campomar” Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 690 | 1 | 0 | |a AURINTRICARBOXYLIC ACID |
| 690 | 1 | 0 | |a GLOBIN |
| 690 | 1 | 0 | |a MAGNESIUM |
| 690 | 1 | 0 | |a MESSENGER RNA |
| 690 | 1 | 0 | |a POLYURIDYLIC ACID |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOSYNTHESIS |
| 690 | 1 | 0 | |a CELL FREE SYSTEM |
| 690 | 1 | 0 | |a DRUG EFFECT |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PEPTIDE SYNTHESIS |
| 690 | 1 | 0 | |a PLANT |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS |
| 690 | 1 | 0 | |a WHEAT |
| 690 | 1 | 0 | |a AURINTRICARBOXYLIC ACID |
| 690 | 1 | 0 | |a CELL-FREE SYSTEM |
| 690 | 1 | 0 | |a GLOBINS |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MAGNESIUM |
| 690 | 1 | 0 | |a PEPTIDE BIOSYNTHESIS |
| 690 | 1 | 0 | |a PLANTS |
| 690 | 1 | 0 | |a POLY U |
| 690 | 1 | 0 | |a PROTEIN BIOSYNTHESIS |
| 690 | 1 | 0 | |a RNA, MESSENGER |
| 690 | 1 | 0 | |a TRITICUM |
| 773 | 0 | |d 1980 |g v. 96 |h pp. 54-60 |k n. 1 |p Top. Catal. |x 0006291x |w (AR-BaUEN)CENRE-905 |t Topics in Catalysis | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-85025574750&doi=10.1016%2f0006-291X%2880%2991180-8&partnerID=40&md5=6ee3ad5f38bd320ac1ba33665f53ae6a |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0006-291X(80)91180-8 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_0006291x_v96_n1_p54_Algranati |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291x_v96_n1_p54_Algranati |y Registro en la Biblioteca Digital |
| 961 | |a paper_0006291x_v96_n1_p54_Algranati |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 77465 | ||