Uridine diphosphate rhamnose from Pisum sativum seeds
Guardado en:
| Autor principal: | |
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| Otros Autores: | |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1967
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 05670caa a22006137a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1638 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203059.0 | ||
| 008 | 190411s1967 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0014220064 | |
| 024 | 7 | |2 cas |a arabinose, 147-81-9; galactose, 26566-61-0, 50855-33-9, 59-23-4; glucose, 50-99-7, 84778-64-3; phosphate, 14066-19-4, 14265-44-2; rhamnose, 10485-94-6, 3615-41-6; uridine, 58-96-8; xylose, 25990-60-7, 58-86-6; Arabinose, 147-81-9; Galactose, 26566-61-0; Glucose, 50-99-7; Phosphates; Rhamnose, 10485-94-6; Uracil Nucleotides; Uridine, 58-96-8; Xylose | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBGSB | ||
| 100 | 1 | |a Vozari Hampe, M.M. | |
| 245 | 1 | 0 | |a Uridine diphosphate rhamnose from Pisum sativum seeds |
| 260 | |c 1967 | ||
| 270 | 1 | 0 | |m Vozari Hampe, M.M.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 536 | |a Detalles de la financiación: National Science Foundation | ||
| 536 | |a Detalles de la financiación: U.S. Public Health Service | ||
| 536 | |a Detalles de la financiación: phates and the tritium exchange reaction, on the evaluation of discrimination by glucose-6-phosphate dehydrogenase would tend to cancel one another. Therefore, we have calculated the extent of discrimination against [I-aH]Gl-c 6-P by glucose-6-phosphate dehydrogenase as in the experiments with the purified enzymes with the realization that the calculation provides only an indication of this discrimination and not an exact estimate (Table II). The value calculated for the quantity of tritiated substrate remaining unoxidized in the experiment with 164 m/~nloles of TPN + was slightly greater than the amount of Glc-6-P added. The difference is within the limits of experimental error (less than 4 %). These calculations show that in the experiments with the mammary gland glucose-6-phosphate dehydrogenase, the first carbon of the tritiated hexose phosphate was not appreciably oxidized until more than 3o °'o (Table II, 82/27o ) of the ~4C-labeled substrate was utilized. Furthermore, the extent of oxidation of the [I-aHqGlc-6-P was only 1/3 that of tile 14C-!abeled hexose phosphate when 45 % (Table II, 12o/27o ) of tile ('-I of the substrate had been oxidized. We, therefore, concur with the conclusions of KATZ et al. 4,~ who state that ~I-aH~glucose should not be the only all-labeled compound used to estimate the pathways of hydrogen in biological systems. This investigation was supported by a grant from tile National Science Foundation to S. A. | ||
| 536 | |a Detalles de la financiación: A furtherp urificationo f a small quantityo f UDP-rhamnoswe as achievedb y treatmenot f the UDP-sugar fraction with glycogens ynthetasfer om rat muscle2° . After precipitationo f the glycogen,t he nucleotidesw ere submittedto descending paperc hromatographiny S olventI , eluteda ndr echromatographtewdic ein Solvent II. A band with a RVhP-glucos:e I.I in SolventsI and II was obtainedT. his R F is the same as that of UDP-[14Clrhamnopsreo ducede nzymaticallbyy incubationo f UDP-Ia4Cjglucoswe ith an enzymicp reparationfr om fresh pea seedsp reparedfo l-lowing the methodd escribedb y BARBER2 . As judged by the behavioro f UDP-rhamnoseon paper chromatographtyh,e sugarn ucleotidep~ e viouslyis olatedb y SMITH, GALLOWAY A~<DM ILLS2 1f romPneumo-coccust ypeII was not the samea s that isolatedf romP isumsativum. The authorsa re gratefult o Dr. LuIs F. LELOIR and other memberso f the Instituto de InvestigacioneBsi oqulmicasfo r supporta nd helpful discussionsT. his investigationw as supportedin part by a researchg rant (No. GM 03442) from the NationalI nstituteso f Health, U.S. Public Health Service,b y the RockefelleFr oun-dation and by the ConsejoN acionald e InvestigacioneCsie ntlficasy Tdcnicas( Repfi-blica Argentina).M .M.V.H. is a fellow of the Campanhad e aperfei~oamendteo pessoadl e nivel superior( Brazil). | ||
| 593 | |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina | ||
| 690 | 1 | 0 | |a ARABINOSE |
| 690 | 1 | 0 | |a GALACTOSE |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a PHOSPHATE |
| 690 | 1 | 0 | |a PYRIMIDINE NUCLEOTIDE |
| 690 | 1 | 0 | |a RHAMNOSE |
| 690 | 1 | 0 | |a URIDINE |
| 690 | 1 | 0 | |a XYLOSE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a PAPER CHROMATOGRAPHY |
| 690 | 1 | 0 | |a PLANT SEED |
| 690 | 1 | 0 | |a ARABINOSE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, PAPER |
| 690 | 1 | 0 | |a GALACTOSE |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a PHOSPHATES |
| 690 | 1 | 0 | |a RHAMNOSE |
| 690 | 1 | 0 | |a SEEDS |
| 690 | 1 | 0 | |a URACIL NUCLEOTIDES |
| 690 | 1 | 0 | |a URIDINE |
| 690 | 1 | 0 | |a XYLOSE |
| 700 | 1 | |a Gonzalez, N.S. | |
| 773 | 0 | |d 1967 |g v. 148 |h pp. 566-568 |k n. 2 |p Biochim. Biophys. Acta Gen. Subj. |x 03044165 |w (AR-BaUEN)CENRE-2260 |t BBA - General Subjects | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0014220064&doi=10.1016%2f0304-4165%2867%2990159-6&partnerID=40&md5=54f8c08351b0627a4dd8480130c7b00c |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0304-4165(67)90159-6 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03044165_v148_n2_p566_VozariHampe |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v148_n2_p566_VozariHampe |y Registro en la Biblioteca Digital |
| 961 | |a paper_03044165_v148_n2_p566_VozariHampe |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 62591 | ||