Some properties of rat liver amylase
Some properties of rat liver amylase were studied. It was confirmed that this enzyme is located mainly in the microsomal fraction and that it is activated by detergents. The effects of digitonin, Triton X-100 and sodium deoxycholate on the amylase were compared. It was observed that amylase requires...
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| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1968
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04817caa a22008417a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1614 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203058.0 | ||
| 008 | 190411s1968 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0014424732 | |
| 024 | 7 | |2 cas |a acid phosphatase, 9001-77-8, 9025-88-1; amylase, 9000-90-2, 9000-92-4, 9001-19-8; sucrose, 122880-25-5, 57-50-1; Acid Phosphatase, EC 3.1.3.2; Amylases, EC 3.2.1.-; Detergents; Liver Glycogen; Sucrose, 57-50-1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ABBIA | ||
| 100 | 1 | |a Mordoh, J. | |
| 245 | 1 | 0 | |a Some properties of rat liver amylase |
| 260 | |c 1968 | ||
| 270 | 1 | 0 | |m Mordoh, J.; Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Some properties of rat liver amylase were studied. It was confirmed that this enzyme is located mainly in the microsomal fraction and that it is activated by detergents. The effects of digitonin, Triton X-100 and sodium deoxycholate on the amylase were compared. It was observed that amylase requires a higher concentration of Triton X-100 for maximal activation than lysosomal acid phosphatase. When lysosomes and microsomes were submitted to a detergent treatment capable of activating completely acid phosphatase and α-amylase, the former was solubilized, while the second remained particulate. The activation of α-amylase was found to be reversible in the first stages. The microsomal α-amylase is not bound to glycogen in fed rats. It was observed that the free α-amylase did not change when the liver was induced to accumulate different amounts of glycogen by administration of sugar, but that latent amylase was doubled when large amounts of glycogen accumulated. The Km of liver amylase for glycogen is 2.5 mg/ml as compared with 0.4 mg/ml for serum amylase. The significance of these observations is discussed. © 1968. |l eng | |
| 536 | |a Detalles de la financiación: National Institutes of Health | ||
| 536 | |a Detalles de la financiación: U.S. Public Health Service | ||
| 536 | |a Detalles de la financiación: Rockefeller Foundation | ||
| 536 | |a Detalles de la financiación: I This investigation was supported in part by a research grant (GM 03442) from the National Institutes of Health, U. S. Public Health Service, by the Rockefeller Foundation, and by the Consejo National de Investigaciones Cientificas y Tecnicas (R. Argentina). ’ Career investigator of the Consejo National de Investigaciones Cientiificas y Tecnicas (R. Argentina). ’ Fellow of the Consejo National de Investiga-ciones Cientificas y Tecnicas (R. Argentina). | ||
| 593 | |a Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina | ||
| 690 | 1 | 0 | |a ACID PHOSPHATASE |
| 690 | 1 | 0 | |a AMYLASE |
| 690 | 1 | 0 | |a DETERGENT |
| 690 | 1 | 0 | |a SUCROSE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BLOOD |
| 690 | 1 | 0 | |a CELL NUCLEUS |
| 690 | 1 | 0 | |a CYTOLOGY |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a FREEZING |
| 690 | 1 | 0 | |a GLYCOGEN LIVER LEVEL |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a LIVER MITOCHONDRION |
| 690 | 1 | 0 | |a LYSOSOME |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MICROSOME |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a STIMULATION |
| 690 | 1 | 0 | |a ACID PHOSPHATASE |
| 690 | 1 | 0 | |a AMYLASES |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CELL NUCLEUS |
| 690 | 1 | 0 | |a DETERGENTS |
| 690 | 1 | 0 | |a FREEZING |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a LIVER GLYCOGEN |
| 690 | 1 | 0 | |a LYSOSOMES |
| 690 | 1 | 0 | |a MICROSOMES |
| 690 | 1 | 0 | |a MITOCHONDRIA, LIVER |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a STIMULATION, CHEMICAL |
| 690 | 1 | 0 | |a SUCROSE |
| 700 | 1 | |a Krisman, C.R. | |
| 700 | 1 | |a Parodi, A.J. | |
| 700 | 1 | |a Leloir, L.F. | |
| 773 | 0 | |d 1968 |g v. 127 |h pp. 193-199 |k n. C |p Arch. Biochem. Biophys. |x 00039861 |w (AR-BaUEN)CENRE-1377 |t Archives of Biochemistry and Biophysics | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0014424732&doi=10.1016%2f0003-9861%2868%2990216-6&partnerID=40&md5=7089f14ca5a6198dbb67e97c1a74fb4c |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0003-9861(68)90216-6 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00039861_v127_nC_p193_Mordoh |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v127_nC_p193_Mordoh |y Registro en la Biblioteca Digital |
| 961 | |a paper_00039861_v127_nC_p193_Mordoh |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 62567 | ||