Luminal Ca2+ dynamics during IP3R mediated signals

The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphate receptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studied at large both experimentally and by modeling. The role of luminal Ca2+ has not been investigated with that much detail although it has been f...

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Autor principal: Lopez, L.F
Otros Autores: Dawson, S.P
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Institute of Physics Publishing 2016
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-84977629192 
024 7 |2 cas  |a calcium, 7440-70-2, 14092-94-5; Calcium; Fluorescent Dyes; Inositol 1,4,5-Trisphosphate Receptors 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Lopez, L.F. 
245 1 0 |a Luminal Ca2+ dynamics during IP3R mediated signals 
260 |b Institute of Physics Publishing  |c 2016 
506 |2 openaire  |e Política editorial 
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520 3 |a The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphate receptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studied at large both experimentally and by modeling. The role of luminal Ca2+ has not been investigated with that much detail although it has been found that it is relevant for signal termination in the case of Ca2+ release through ryanodine receptors. In this work we present the results of observing the dynamics of luminal and cytosolic Ca2+ simultaneously in Xenopus laevis oocytes. Combining observations and modeling we conclude that there is a rapid mechanism that guarantees the availability of free Ca2+ in the lumen even when a relatively large Ca2+ release is evoked. Comparing the dynamics of cytosolic and luminal Ca2+ during a release, we estimate that they are consistent with a 80% of luminal Ca2+ being buffered. The rapid availability of free luminal Ca2+ correlates with the observation that the lumen occupies a considerable volume in several regions across the images. © 2016 IOP Publishing Ltd.  |l eng 
593 |a DF, FCEN-UBA, Argentina 
593 |a IFIBA, CONICET, Argentina 
690 1 0 |a CALCIUM 
690 1 0 |a FLUORESCENT DYE 
690 1 0 |a INOSITOL 1,4,5 TRISPHOSPHATE RECEPTOR 
690 1 0 |a ANIMAL 
690 1 0 |a CALCIUM SIGNALING 
690 1 0 |a CHEMISTRY 
690 1 0 |a CYTOSOL 
690 1 0 |a METABOLISM 
690 1 0 |a OOCYTE 
690 1 0 |a XENOPUS LAEVIS 
690 1 0 |a ANIMALS 
690 1 0 |a CALCIUM 
690 1 0 |a CALCIUM SIGNALING 
690 1 0 |a CYTOSOL 
690 1 0 |a FLUORESCENT DYES 
690 1 0 |a INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS 
690 1 0 |a OOCYTES 
690 1 0 |a XENOPUS LAEVIS 
700 1 |a Dawson, S.P. 
773 0 |d Institute of Physics Publishing, 2016  |g v. 13  |k n. 3  |p Phys. Biol.  |x 14783967  |t Physical Biology 
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