In vitro synthesis of particulate glycogen from uridine diphosphate glucose. II. Some studies on the growth process

It was found that for the same extent of synthesis, the glycogen synthesized in vitro by liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) from uridine diphosphate glucose is heavier than that synthesized from glucose 1-phosphate by muscle pho...

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Autor principal: Parodi, A.J
Otros Autores: Krisman, C.R, Mordoh, J.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1970
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 cas  |a glucosyltransferase, 9031-48-5; glycogen, 9005-79-2; Glucosyltransferases, EC 2.4.1.-; Glycogen, 9005-79-2; Hexosephosphates; Liver Glycogen; Uracil Nucleotides 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a ABBIA 
100 1 |a Parodi, A.J. 
245 1 3 |a In vitro synthesis of particulate glycogen from uridine diphosphate glucose. II. Some studies on the growth process 
260 |c 1970 
270 1 0 |m Parodi, A.J.; Institute de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires 28, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Mordoh, Leloir, Krisman, (1965) Proc. Nat. Acad. Sci. U. S. A, 53, p. 86. , 1st. Ed 
504 |a Parodi, Mordoh, Krisman, Leloir, (1969) Arch. Biochem. Biophys, 132, p. 111 
504 |a Krisman, Parodi, (1970) Arch. Biochim. Biophys, 141, p. 228 
504 |a Mordoh, Krisman, Leloir, (1966) Arch. Biochem. Biophys, 113, p. 265 
504 |a Parodi, Krisman, Leloir, Mordoh, (1967) Arch. Biochem. Biophys, 121, p. 769 
504 |a Sahyun, Alsberg, (1930) J. Biol. Chem, 89, p. 33 
504 |a Orrell, Bueding, (1964) J. Biol. Chem, 239, p. 4021 
504 |a Stetten, Stetten, (1958) Third Conference on Polysaccharides in Biology, p. 9. , G.F. Springer, Madison Printing Co., Inc., Madison, Wisconsin 
504 |a Dixon, Webb, (1958) Enzymes, p. 91. , 1st. Ed., Longmans, Green, London 
504 |a Rothman, Gabib, (1967) Biochemistry, 6, p. 2098 
520 3 |a It was found that for the same extent of synthesis, the glycogen synthesized in vitro by liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) from uridine diphosphate glucose is heavier than that synthesized from glucose 1-phosphate by muscle phosphorylase b (α-1,4 glucan:orthophosphate glucosyltransferase, EC 2.4.1.1). This result could be due to the different specificity of both transferring enzymes toward the molecular weight of the acceptor glycogen and to the incomplete use of the primer by glycogen synthetase. Glycogen synthesized from uridine diphosphate glucose showed a single light population in the first stages of synthesis. Later on, a clearly distinguishable heavier population appeared while the initial light peak was slightly shifted toward a higher molecular weight. The synthesis of glycogen from glucose 1-phosphate proceeded in a rather different way: only a single peak appeared during the synthesis. The molecular weight of this population increased as synthesis went on. An aggregation of the molecules of glycogen during synthesis from uridine diphosphate glucose could explain some of the results obtained. © 1970.  |l eng 
536 |a Detalles de la financiación: T6cnicas 
536 |a Detalles de la financiación: National Institutes of Health 
536 |a Detalles de la financiación: U.S. Public Health Service 
536 |a Detalles de la financiación: 1 This investigation was supported in part by Research Grant GM 03442 from the National Institutes of Health, U. S. Public Health Service, and by the Consejo National de Investigaciones Cientificas y T6cnicas (R. Argentina). Paper I in this series is Ref. 2. 2 Career investigator of the Consejo National de Investigaciones Cientificas y T6cnicas. 3 The following abbreviations are used: glc-l-P, d-glucose l-phosphate; UDPG, uridine diphosphate glucose. 
593 |a Institute de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires 28, Argentina 
690 1 0 |a GLUCOSYLTRANSFERASE 
690 1 0 |a GLYCOGEN 
690 1 0 |a HEXOSE PHOSPHATE 
690 1 0 |a PYRIMIDINE NUCLEOTIDE 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a BIOSYNTHESIS 
690 1 0 |a ENZYMOLOGY 
690 1 0 |a GLYCOGEN LIVER LEVEL 
690 1 0 |a KINETICS 
690 1 0 |a LIVER 
690 1 0 |a METABOLISM 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a MOUSE 
690 1 0 |a MUSCLE 
690 1 0 |a ULTRACENTRIFUGATION 
690 1 0 |a ANIMAL 
690 1 0 |a GLUCOSYLTRANSFERASES 
690 1 0 |a GLYCOGEN 
690 1 0 |a HEXOSEPHOSPHATES 
690 1 0 |a KINETICS 
690 1 0 |a LIVER 
690 1 0 |a LIVER GLYCOGEN 
690 1 0 |a MICE 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a MUSCLES 
690 1 0 |a ULTRACENTRIFUGATION 
690 1 0 |a URACIL NUCLEOTIDES 
700 1 |a Krisman, C.R. 
700 1 |a Mordoh, J. 
773 0 |d 1970  |g v. 141  |h pp. 219-227  |k n. 1  |p Arch. Biochem. Biophys.  |x 00039861  |w (AR-BaUEN)CENRE-1377  |t Archives of Biochemistry and Biophysics 
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