In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase

High molecular weight glycogen has been prepared in vitro with muscle glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and liver or muscle branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) with uridine diphosphate glu...

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Detalles Bibliográficos
Autor principal: Krisman, C.R
Otros Autores: Parodi, A.J
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1970
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
LEADER 05781caa a22008897a 4500
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008 190411s1970 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0014876210 
024 7 |2 cas  |a glucose, 50-99-7, 84778-64-3; glucosyltransferase, 9031-48-5; glycogen, 9005-79-2; phosphate, 14066-19-4, 14265-44-2; sodium chloride, 7647-14-5; sulfate, 14808-79-8; Ammonium Compounds; Glucose, 50-99-7; Glucosyltransferases, EC 2.4.1.-; Glycogen, 9005-79-2; Liver Glycogen; Phosphates; Sodium Chloride, 7647-14-5; Sulfates; Uracil Nucleotides 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a ABBIA 
100 1 |a Krisman, C.R. 
245 1 3 |a In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase 
260 |c 1970 
270 1 0 |m Krisman, C.R.; Institute de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires 28, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Bueding, Orrell, Sidbury, (1964) Ciba Foundation Symposium on Control of Glycogen Metabolism, p. 387. , W.J. Whelan, M.P. Cameron, Churchill, London 
504 |a Orrell, Bueding, Reissig, (1964) Ciba Foundation Symposium on Control of Glycogen Metabolism, p. 29. , W.J. Whelan, M.P. Cameron, Churchill, London 
504 |a Parodi, Krisman, Leloir, Mordoh, (1967) Arch. Biochem. Biophys, 121, p. 769 
504 |a Barber, Harris, Anderson, (1966) Nat. Cancer Inst. Monogr, 21, p. 287 
504 |a Mordoh, Krisman, Leloir, (1966) Arch. Biochem. Biophys, 113, p. 265 
504 |a Parodi, A. J., unpublished results; Parodi, Mordoh, Krisman, Leloir, (1969) Arch. Biochem. Biophys, 132, p. 111 
504 |a Mordoh, Leloir, Krisman, (1965) Proc. Nat. Acad. Sci. U. S. A, 53, p. 86 
504 |a Parodi, Krisman, Mordoh, (1970) Arch. Biochem. Biophys, 141, p. 219 
504 |a Krisman, (1962) Biochim. Biophys. Acta, 65, p. 307 
504 |a Krisman, (1962) Anal. Biochem, 4, p. 17 
520 3 |a High molecular weight glycogen has been prepared in vitro with muscle glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and liver or muscle branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) with uridine diphosphate glucose as glucose donor. The product obtained with a fresh synthetase preparation had a relatively low molecular weight like native muscle glycogen. The product synthesized with an aged synthetase preparation had a very high molecular weight similar to that of native liver glycogen. Ammonium sulfate and other salts inhibited the formation of high molecular weight glycogen by the aged synthetase preparation but not the total synhesis of the polysaccharide. The effect of the salts on the molecular weight of the product synthesized by the liver synthetase was much smaller. The changes in the molecular weight distribution of glycogen during synthesis with fresh, aged, and aged plus ammonium sulfate glycogen synthetases were studied. The results obtained are discussed. © 1970.  |l eng 
536 |a Detalles de la financiación: National Institutes of Health 
536 |a Detalles de la financiación: U.S. Public Health Service 
536 |a Detalles de la financiación: i This investigation was supported in part by Research Grant GM 03442 from the National Institutes of Health, U. S. Public Health Service, and by the Consejo National de Investigaciones Cientificas y Tdcnicas (R. Argentina). Paper II in this series is Ref. 9. 
536 |a Detalles de la financiación: 2 Career investigator of the Consejo National de Investigaciones Cientificas y Tecnicas. 
593 |a Institute de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires 28, Argentina 
690 1 0 |a AMMONIUM DERIVATIVE 
690 1 0 |a GLUCOSE 
690 1 0 |a GLUCOSYLTRANSFERASE 
690 1 0 |a GLYCOGEN 
690 1 0 |a PHOSPHATE 
690 1 0 |a PYRIMIDINE NUCLEOTIDE 
690 1 0 |a SODIUM CHLORIDE 
690 1 0 |a SULFATE 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a BIOSYNTHESIS 
690 1 0 |a ENZYMOLOGY 
690 1 0 |a GLYCOGEN LIVER LEVEL 
690 1 0 |a LIVER 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a MUSCLE 
690 1 0 |a RAT 
690 1 0 |a TIME 
690 1 0 |a AMMONIUM COMPOUNDS 
690 1 0 |a ANIMAL 
690 1 0 |a GLUCOSE 
690 1 0 |a GLUCOSYLTRANSFERASES 
690 1 0 |a GLYCOGEN 
690 1 0 |a LIVER 
690 1 0 |a LIVER GLYCOGEN 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a MUSCLES 
690 1 0 |a PHOSPHATES 
690 1 0 |a RATS 
690 1 0 |a SODIUM CHLORIDE 
690 1 0 |a SULFATES 
690 1 0 |a TIME FACTORS 
690 1 0 |a URACIL NUCLEOTIDES 
700 1 |a Parodi, A.J. 
773 0 |d 1970  |g v. 141  |h pp. 228-235  |k n. 1  |p Arch. Biochem. Biophys.  |x 00039861  |w (AR-BaUEN)CENRE-1377  |t Archives of Biochemistry and Biophysics 
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