Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase an...
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2016
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| 003 | AR-BaUEN | ||
| 005 | 20230518204627.0 | ||
| 008 | 190411s2016 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84976898446 | |
| 024 | 7 | |2 cas |a lipoteichoic acid, 56411-57-5; peptidoglycan, 9047-10-3; protein, 67254-75-5; sodium chloride, 7647-14-5; Membrane Glycoproteins; S-layer proteins; Sodium Chloride | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a AMBID | ||
| 100 | 1 | |a Palomino, M.M. | |
| 245 | 1 | 0 | |a Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356 |
| 260 | |b Springer Verlag |c 2016 | ||
| 270 | 1 | 0 | |m Ruzal, S.M.; Laboratorio Bacterias Gram Positivas, sus Fagos y Estrés, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales-UBA, IQUIBICEN-CONICET, Ciudad Universitaria, Pabellon II, piso 4, Argentina; email: sandra@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase and high-salt conditions (0.6 M NaCl). Changes in gene expression were also observed as the mRNAs of the slpA and slpX genes increased related to the growth phase and presence of high salt. A growth stage-dependent modification on the S-layer protein profile in response to NaCl was observed: while in control conditions, the auxiliary SlpX protein represented less than 10 % of the total S-layer protein, in high-salt conditions, it increased to almost 40 % in the stationary phase. The increase in S-layer protein synthesis in the stress condition could be a consequence of or a way to counteract the fragility of the cell wall, since a decrease in the cell wall thickness and envelope components (peptidoglycan layer and lipoteichoic acid content) was observed in L. acidophilus when compared to a non-S-layer-producing species such as Lactobacillus casei. Also, the stationary phase and growth in high-salt medium resulted in increased release of S-layer proteins to the supernatant medium. Overall, these findings suggest that pre-growth in high-salt conditions would result in an advantage for the probiotic nature of L. acidophilus ATCC 4356 as the increased amount and release of the S-layer might be appropriate for its antimicrobial capacity. © 2016, Springer-Verlag Berlin Heidelberg. |l eng | |
| 593 | |a Laboratorio Bacterias Gram Positivas, sus Fagos y Estrés, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales-UBA, IQUIBICEN-CONICET, Ciudad Universitaria, Pabellon II, piso 4, Buenos Aires, Ciudad de Buenos Aires 1428, Argentina | ||
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| 650 | 1 | 7 | |2 spines |a OSMOSIS |
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| 700 | 1 | |a Waehner, P.M. | |
| 700 | 1 | |a Fina Martin, J. | |
| 700 | 1 | |a Ojeda, P. | |
| 700 | 1 | |a Malone, L. | |
| 700 | 1 | |a Sánchez Rivas, C. | |
| 700 | 1 | |a Prado Acosta, M. | |
| 700 | 1 | |a Allievi, M.C. | |
| 700 | 1 | |a Ruzal, S.M. | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01757598_v100_n19_p8475_Palomino |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v100_n19_p8475_Palomino |y Registro en la Biblioteca Digital |
| 961 | |a paper_01757598_v100_n19_p8475_Palomino |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 76635 | ||