Monitoring in real-time focal adhesion protein dynamics in response to a discrete mechanical stimulus

Mostrar otras versiones (2)

The adhesion of cells to the extracellular matrix is a hierarchical, force-dependent, multistage process that evolves at several temporal scales. An understanding of this complex process requires a precise measurement of forces and its correlation with protein responses in living cells. We present a...

Descripción completa

Detalles Bibliográficos
Autor principal: Von Bilderling, C.
Otros Autores: Caldarola, M., Masip, M.E, Bragas, Andrea Verónica, Pietrasanta, L.I
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: American Institute of Physics Inc. 2017
Materias:
ADHESION
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
LEADER 08572caa a22011417a 4500
001 PAPER-15337
003 AR-BaUEN
005 20241029142439.0
008 190410s2017 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-85009469926 
024 7 |2 cas  |a protein, 67254-75-5; Proteins 
030 |a RSINA 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Von Bilderling, C. 
245 1 0 |a Monitoring in real-time focal adhesion protein dynamics in response to a discrete mechanical stimulus 
260 |b American Institute of Physics Inc.  |c 2017 
270 1 0 |m Bragas, A.V.; IFIBA-CONICET-UBAArgentina; email: bragas@df.uba.ar 
504 |a Chen, C.S., (2008) J. Cell Sci., 121, p. 3285 
504 |a Burridge, K., Guilluy, C., (2016) Exp. Cell Res., 343, p. 14 
504 |a Livne, A., Geiger, B., (2016) J. Cell Sci., 129, p. 1293 
504 |a Zaidel-Bar, R., Itzkovitz, S., Ma’ayan, A., Iyengar, R., Geiger, B., (2007) Nat. Cell Biol., 9, p. 858 
504 |a Zamir, E., Geiger, B., (2001) J. Cell Sci., 114, p. 3583 
504 |a Kanchanawong, P., Shtengel, G., Pasapera, A.M., Ramko, E.B., Davidson, M.W., Hess, H.F., Waterman, C.M., (2010) Nature, 468, p. 580 
504 |a Geiger, B., Bershadsky, A., Pankov, R., Yamada, K.M., (2001) Nat. Rev. Mol. Cell Biol., 2, p. 793 
504 |a Davies, P.F., Robotewskyj, A., Griem, M.L., (1994) J. Clin. Invest., 93, p. 2031 
504 |a Goldyn, A.M., Rioja, B.A., Spatz, J.P., Ballestrem, C., Kemkemer, R., (2009) J. Cell Sci., 122, p. 3644 
504 |a Schwingel, M., Bastmeyer, M., (2013) PLoS One, 8, p. e54850 
504 |a Galbraith, C.G., Yamada, K.M., Sheetz, M.P., (2002) J. Cell Biol., 159, p. 695 
504 |a Yung, Y.C., Chae, J., Buehler, M.J., Hunter, C.P., Mooney, D.J., (2009) Proc. Natl. Acad. Sci. U. S. A., 106, p. 15279 
504 |a Yoshigi, M., Hoffman, L.M., Jensen, C.C., Yost, H.J., Beckerle, M.C., (2005) J. Cell Biol., 171, p. 209 
504 |a Rotsch, C., Radmacher, M., (2000) Biophys. J., 78, p. 520 
504 |a Hinterdorfer, P., Dufrene, Y.F., (2006) Nat. Methods, 3, p. 347 
504 |a Muller, D.J., Helenius, J., Alsteens, D., Dufrene, Y.F., (2009) Nat. Chem. Biol., 5, p. 383 
504 |a Charras, G.T., Horton, M.A., (2002) Biophys. J., 82, p. 2970 
504 |a Shao, X., Li, Q., Mogilner, A., Bershadsky, A.D., Shivashankar, G.V., (2015) Proc. Natl. Acad. Sci. U. S. A., 112, p. E2595 
504 |a Lim, S.M., Trzeciakowski, J.P., Sreenivasappa, H., Dangott, L.J., Trache, A., (2012) Integr. Biol., 4, p. 615 
504 |a Chaudhuri, O., Parekh, S.H., Lam, W.A., Fletcher, D.A., (2009) Nat. Methods, 6, p. 383 
504 |a Caldarola, M., Von Bilderling, C., Masip, M.E., Pietrasanta, L.I., Bragas, A.V., (2012) Proceedings of Frontiers in Optics 2012/Laser Science XXVIII, , Optical Society of America 
504 |a Balaban, N.Q., Schwarz, U.S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., Mahalu, D., Geiger, B., (2001) Nat. Cell Biol., 3, p. 466 
504 |a Matthews, B.D., Overby, D.R., Alenghat, F.J., Karavitis, J., Numaguchi, Y., Allen, P.G., Ingber, D.E., (2004) Biochem. Biophys. Res. Commun., 313, p. 758 
504 |a Riveline, D., Zamir, E., Balaban, N.Q., Schwarz, U.S., Ishizaki, T., Narumiya, S., Kam, Z., Bershadsky, A.D., (2001) J. Cell Biol., 153, p. 1175 
504 |a Tamada, M., Sheetz, M.P., Sawada, Y., (2004) Dev. Cell, 7, p. 709 
504 |a Wang, N., Butler, J.P., Ingber, D.E., (1993) Science, 260, p. 1124 
504 |a Zamir, E., Katz, M., Posen, Y., Erez, N., Yamada, K.M., Katz, B.Z., Lin, S., Geiger, B., (2000) Nat. Cell Biol., 2, p. 191 
504 |a Hutter, J.L., Bechhoefer, B.J., (1993) Rev. Sci. Instrum., 64, p. 1868 
504 |a Ball, R.K., Friis, R.R., Schoenenberger, C.A., Doppler, W., Groner, B., (1988) EMBO J., 7, p. 2089 
504 |a Lawson, C., Schlaepfer, D.D., (2012) Cell Adhes. Migr., 6, p. 302 
504 |a Hoffmann, J.E., Fermin, Y., Stricker, R.L., Ickstadt, K., Zamir, E., (2014) eLife, 3, p. e02257 
504 |a Mierke, C.T., (2013) Phys. Biol., 10, p. 065005 
504 |a Schaller, M.D., (2001) Oncogene, 20, p. 6459 
504 |a Laukaitis, C.M., Webb, D.J., Donais, K., Horwitz, A.F., (2001) J. Cell Biol., 153, p. 1427 
504 |a Von Wichert, G., Haimovich, B., Feng, G.S., Sheetz, M.P., (2003) EMBO J., 22, p. 5023 
504 |a Zaidel-Bar, R., Ballestrem, C., Kam, Z., Geiger, B., (2003) J. Cell Sci., 116, p. 4605 
504 |a Winograd-Katz, S.E., Fassler, R., Geiger, B., Legate, K.R., (2014) Nat. Rev. Mol. Cell Biol., 15, p. 273 
504 |a Yu, C.H., Law, J.B., Suryana, M., Low, H.Y., Sheetz, M.P., (2011) Proc. Natl. Acad. Sci. U. S. A., 108, p. 20585 
504 |a Sun, Z., Huang, S., Li, Z., Meininger, G.A., (2012) Front. Physiol., 3, p. 472 
504 |a Hoffman, B.D., Grashoff, C., Schwartz, M.A., (2011) Nature, 475, p. 316 
506 |2 openaire  |e Política editorial 
520 3 |a The adhesion of cells to the extracellular matrix is a hierarchical, force-dependent, multistage process that evolves at several temporal scales. An understanding of this complex process requires a precise measurement of forces and its correlation with protein responses in living cells. We present a method to quantitatively assess live cell responses to a local and specific mechanical stimulus. Our approach combines atomic force microscopy with fluorescence imaging. Using this approach, we evaluated the recruitment of adhesion proteins such as vinculin, focal adhesion kinase, paxillin, and zyxin triggered by applying forces in the nN regime to live cells. We observed in real time the development of nascent adhesion sites, evident from the accumulation of early adhesion proteins at the position where the force was applied. We show that the method can be used to quantify the recruitment characteristic times for adhesion proteins in the formation of focal complexes. We also found a spatial remodeling of the mature focal adhesion protein zyxin as a function of the applied force. Our approach allows the study of a variety of complex biological processes involved in cellular mechanotransduction. © 2017 Author(s).  |l eng 
593 |a Centro de Microscopías Avanzadas, Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a IFIBA-CONICET-UBA, Buenos Aires, Argentina 
593 |a Laboratorio de Electrónica Cuántica, Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina 
593 |a Leiden Institute of Physics, Leiden University, Netherlands 
593 |a Max Planck Institute of Molecular Physiology, Dortmund, Germany 
650 1 7 |2 spines  |a ADHESION 
690 1 0 |a ATOMIC FORCE MICROSCOPY 
690 1 0 |a CELLS 
690 1 0 |a CYTOLOGY 
690 1 0 |a PROTEINS 
690 1 0 |a BIOLOGICAL PROCESS 
690 1 0 |a EXTRACELLULAR MATRICES 
690 1 0 |a FLUORESCENCE IMAGING 
690 1 0 |a FOCAL ADHESION KINASE 
690 1 0 |a MECHANICAL STIMULUS 
690 1 0 |a MECHANOTRANSDUCTION 
690 1 0 |a MULTISTAGE PROCESS 
690 1 0 |a PRECISE MEASUREMENTS 
690 1 0 |a PROTEIN 
690 1 0 |a CELL FUNCTION 
690 1 0 |a CHEMISTRY 
690 1 0 |a FOCAL ADHESION 
690 1 0 |a MECHANOTRANSDUCTION 
690 1 0 |a CELL PHYSIOLOGICAL PHENOMENA 
690 1 0 |a FOCAL ADHESIONS 
690 1 0 |a MECHANOTRANSDUCTION, CELLULAR 
690 1 0 |a PROTEINS 
700 1 |a Caldarola, M. 
700 1 |a Masip, M.E. 
700 1 |a Bragas, Andrea Verónica 
700 1 |a Pietrasanta, L.I. 
773 0 |d American Institute of Physics Inc., 2017  |g v. 88  |k n. 1  |p Rev. Sci. Instrum.  |x 00346748  |w (AR-BaUEN)CENRE-399  |t Review of Scientific Instruments 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009469926&doi=10.1063%2f1.4973664&partnerID=40&md5=9378e856acd12eec987722c4a39b3dbe  |x registro  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1063/1.4973664  |x doi  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_00346748_v88_n1_p_VonBilderling  |x handle  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00346748_v88_n1_p_VonBilderling  |x registro  |y Registro en la Biblioteca Digital 
961 |a paper_00346748_v88_n1_p_VonBilderling  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 

Ejemplares similares