Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice

Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies w...

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Autor principal: Sóñora, C.
Otros Autores: Mourglia-Ettlin, G., Calo, G., Hauk, V., Ramhorst, R., Hernández, A., Leirós, C.P
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Elsevier Ireland Ltd 2014
Acceso en línea:Registro en Scopus
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024 7 |2 cas  |a immunoglobulin G, 97794-27-9; protein glutamine gamma glutamyltransferase, 80146-85-6; Antibodies; Autoantigens; GTP-Binding Proteins; Immunoglobulin G; transglutaminase 2; Transglutaminases 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a JRIMD 
100 1 |a Sóñora, C. 
245 1 0 |a Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice 
260 |b Elsevier Ireland Ltd  |c 2014 
270 1 0 |m Sóñora, C.; Cátedra de Inmunología, Facultad de Ciencias/Facultad de Química, Universidad de la República, Instituto de Higiene, Alfredo Navarro 3051, CP 11600, Montevideo, Uruguay; email: csonora@fmed.edu.uy 
506 |2 openaire  |e Política editorial 
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520 3 |a Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies were detected in a non-obese diabetic (NOD) mouse model of type I insulin-dependent diabetes mellitus and Sjögren's syndrome, two comorbid states with CD. Since pregnancy complications have been described in NOD mice, we evaluated the ability of anti-tTG antibodies to affect the functions of tTG relevant to the normal course of an early pregnancy like extracellular matrix assembling and apoptotic cell phagocytosis by macrophages. Circulating IgG antibodies against tTG were detected in NOD mice with titers that decreased at early pregnancy; interestingly, the in vitro transamidating activity of tTG was reduced by NOD serum samples. Particularly, anti-tTG antibody inhibited apoptotic cell phagocytosis by peritoneal macrophages from pregnant NOD mice that express the enzyme on surface. Evidence provided support for a role for anti-tTG antibodies through reduced transamidating activity and reduced apoptotic cell clearance by the macrophages of pregnant NOD mice. © 2013 Elsevier Ireland Ltd.  |l eng 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, UBACyT 2012–2015 20020090200034 
536 |a Detalles de la financiación: Agencia Nacional de Investigación e Innovación 
536 |a Detalles de la financiación: We acknowledge Dr. Roberto Meiss and Dr. Verónica Beovide for collaboration in histological analysis and image acquisition. This study was supported by grants to C.P.L. (UBACyT 2011–2014 20020100100505 and PICT 2011-0144 from ANPCyT ), grant to R.R. (UBACyT 2012–2015 20020090200034) and partial support from PEDECIBA (Programa de Desarrollo de las Ciencias Básicas, PNUD), ANII (Agencia Nacional de Investigación e Innovación) and ESCALA program for teachers exchange of AUGM (Asociación de Universidades del Grupo Montevideo). 
593 |a Cátedra de Inmunología, Facultad de Ciencias/Facultad de Química, Universidad de la República, Montevideo, Uruguay 
593 |a Escuela Universitaria de Tecnología Médica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay 
593 |a Laboratorio de Inmunofarmacología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina 
690 1 0 |a ANTI-TISSUE TRANSGLUTAMINASE ANTIBODIES 
690 1 0 |a CELIAC DISEASE 
690 1 0 |a NOD MICE 
690 1 0 |a AUTOANTIBODY 
690 1 0 |a IMMUNOGLOBULIN A 
690 1 0 |a IMMUNOGLOBULIN G 
690 1 0 |a IMMUNOGLOBULIN G ANTIBODY 
690 1 0 |a PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 
690 1 0 |a PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE ANTIBODY 
690 1 0 |a ANTIBODY 
690 1 0 |a AUTOANTIGEN 
690 1 0 |a GUANINE NUCLEOTIDE BINDING PROTEIN 
690 1 0 |a IMMUNOGLOBULIN G 
690 1 0 |a PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 
690 1 0 |a PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 2 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a APOPTOSIS 
690 1 0 |a ARTICLE 
690 1 0 |a CELIAC DISEASE 
690 1 0 |a CELL PHAGOCYTOSIS 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a ENZYME ACTIVITY 
690 1 0 |a ENZYME LINKED IMMUNOSORBENT ASSAY 
690 1 0 |a EXTRACELLULAR MATRIX 
690 1 0 |a FEMALE 
690 1 0 |a FIRST TRIMESTER PREGNANCY 
690 1 0 |a IMMUNOFLUORESCENCE 
690 1 0 |a IMMUNOHISTOCHEMISTRY 
690 1 0 |a MALE 
690 1 0 |a MOUSE 
690 1 0 |a NONHUMAN 
690 1 0 |a NONOBESE DIABETIC MOUSE 
690 1 0 |a PERITONEUM MACROPHAGE 
690 1 0 |a PREGNANCY 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a ANIMAL 
690 1 0 |a APOPTOSIS 
690 1 0 |a BAGG ALBINO MOUSE 
690 1 0 |a BLOOD 
690 1 0 |a IMMUNOLOGY 
690 1 0 |a INSULIN DEPENDENT DIABETES MELLITUS 
690 1 0 |a MACROPHAGE 
690 1 0 |a PHAGOCYTOSIS 
690 1 0 |a PREGNANCY 
690 1 0 |a PREGNANCY OUTCOME 
690 1 0 |a SJOEGREN SYNDROME 
690 1 0 |a UTERUS 
690 1 0 |a ANIMALS 
690 1 0 |a ANTIBODIES 
690 1 0 |a APOPTOSIS 
690 1 0 |a AUTOANTIGENS 
690 1 0 |a CELIAC DISEASE 
690 1 0 |a DIABETES MELLITUS, TYPE 1 
690 1 0 |a FEMALE 
690 1 0 |a GTP-BINDING PROTEINS 
690 1 0 |a IMMUNOGLOBULIN G 
690 1 0 |a MACROPHAGES 
690 1 0 |a MICE 
690 1 0 |a MICE, INBRED BALB C 
690 1 0 |a MICE, INBRED NOD 
690 1 0 |a PHAGOCYTOSIS 
690 1 0 |a PREGNANCY 
690 1 0 |a PREGNANCY OUTCOME 
690 1 0 |a SJOGREN'S SYNDROME 
690 1 0 |a TRANSGLUTAMINASES 
690 1 0 |a UTERUS 
700 1 |a Mourglia-Ettlin, G. 
700 1 |a Calo, G. 
700 1 |a Hauk, V. 
700 1 |a Ramhorst, R. 
700 1 |a Hernández, A. 
700 1 |a Leirós, C.P. 
773 0 |d Elsevier Ireland Ltd, 2014  |g v. 103  |h pp. 59-66  |k n. 1  |p J. Reprod. Immunol.  |x 01650378  |t Journal of Reproductive Immunology 
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