Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique
A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic a...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1975
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04745caa a22007937a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1415 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203046.0 | ||
| 008 | 190411s1975 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0016515151 | |
| 024 | 7 | |2 cas |a carbon, 7440-44-0; uridine diphosphate glucose, 133-89-1; Carbon Radioisotopes; Glycoproteins; Uridine Diphosphate Glucose, 133-89-1; Uridine Diphosphate Sugars | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ANBCA | ||
| 100 | 1 | |a Krisman, C.R. | |
| 245 | 1 | 0 | |a Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| 260 | |c 1975 | ||
| 270 | 1 | 0 | |m Krisman, C.R.; Instituto de Investigaciones Bioquimicas Fundacion Campomar, Obligado, 2490 Buenos Aires (28), Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Bollum, (1959) J. Biol. Chem, 234, p. 2733 | ||
| 504 | |a Bollum, (1968) Methods in Enzymology, 12, p. 169. , S.P. Colowick, N.O. Kaplan, Academic Press, New York | ||
| 504 | |a Mans, Novelli, (1961) Arch. Biochem. Biophys, 94, p. 48 | ||
| 504 | |a Thomas, Schlender, Larner, (1968) Anal. Biochem, 25, p. 486 | ||
| 504 | |a Byfield, Scherbaum, (1966) Anal. Biochem, 17, p. 434 | ||
| 504 | |a Krisman, Barengo, (1975) Eur. J. Biochem, 52, p. 117 | ||
| 504 | |a Krisman, (1973) Ann. N.Y. Acad. Sci, 210, p. 81 | ||
| 504 | |a Rothman-Denes, Muthukrishnan, Haselkorn, Studier, (1973) Virus Research, p. 227. , C.F. Fox, W.S. Robinson, Academic Press, New York | ||
| 504 | |a Wright, Robbins, (1965) Biochim. Biophys. Acta, 104, p. 594 | ||
| 504 | |a Krisman, (1972) Biochem. Biophys. Res. Commun, 46, p. 1206 | ||
| 520 | 3 | |a A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975. |l eng | |
| 536 | |a Detalles de la financiación: National Institutes of Health | ||
| 536 | |a Detalles de la financiación: The authors are grateful to Dr. Luis F. Leloir for advice and support and to other members of the Institute de lnvestigaciones Bioquimicas for helpful discussions and criticisms. This investigation was supported in part by grants from the Ministerio de Bienestar Social, Consejo National de lnvestigaciones Cientificas y Tt%nicas (Argentina) and National Institutes of Health. C.R.K. is a career investigator and R.B. a fellow of the Consejo National de Investigaciones Cientificas y Tbcnicas. | ||
| 593 | |a Instituto de Investigaciones Bioquimicas Fundacion Campomar, Obligado, 2490 Buenos Aires (28), Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina | ||
| 650 | 1 | 7 | |2 spines |a CARBON |
| 690 | 1 | 0 | |a GLYCOPROTEIN |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE GLUCOSE |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE SUGAR |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOSYNTHESIS |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PAPER |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a TECHNIQUE |
| 690 | 1 | 0 | |a TIME |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CARBON RADIOISOTOPES |
| 690 | 1 | 0 | |a EVALUATION STUDIES |
| 690 | 1 | 0 | |a GLYCOPROTEINS |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a METHODS |
| 690 | 1 | 0 | |a PAPER |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a TIME FACTORS |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE GLUCOSE |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE SUGARS |
| 700 | 1 | |a Barengo, R. | |
| 773 | 0 | |d 1975 |g v. 66 |h pp. 584-589 |k n. 2 |p Anal. Biochem. |x 00032697 |w (AR-BaUEN)CENRE-163 |t Analytical Biochemistry | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0016515151&doi=10.1016%2f0003-2697%2875%2990625-9&partnerID=40&md5=7f7b612ec1b84cb01a0edbbdef6382ab |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0003-2697(75)90625-9 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v66_n2_p584_Krisman |y Registro en la Biblioteca Digital |
| 961 | |a paper_00032697_v66_n2_p584_Krisman |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 62368 | ||