Improved ROS measurement in root hair cells

Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress respon...

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Autor principal: Juárez, S.P.D
Otros Autores: Mangano, S., Estevez, J.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer New York 2014
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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100 1 |a Juárez, S.P.D. 
245 1 0 |a Improved ROS measurement in root hair cells 
260 |b Springer New York  |c 2014 
270 1 0 |m Juárez, S.P.D.; IFIByNE (CONICET), FCEyN, Universidad de Buenos AiresArgentina 
506 |2 openaire  |e Política editorial 
504 |a Suzuki, N., Miller, G., Morales, J., Shulaev, V., Torres, M., Mittler, R., Respiratory burst oxidases: the engines of ROS signaling (2011) Plant Biol, 14, pp. 691-699 
504 |a Roppolo, D., De Rybel, B., Tendon, V., Pfister, A., Alassimone, J., Vermeer, J., Yamazaki, M., Geldner, N., A novel protein family mediates Casparian strip formation in the endodermis (2011) Nature, 473, pp. 380-384 
504 |a Takeda, S., Gapper, C., Kaya, H., Bell, E., Kuchitsu, K., Dolan, L., Local positive feedback regulation determines cell shape in root hair cells (2008) Science, 319, pp. 1241-1244 
504 |a Potocký, M., Jones, M., Bezvoda, R., Smirnoff, N., Zárský, V., Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth (2007) New Phytol, 174, pp. 742-751 
504 |a Foreman, J., Demidchik, V., Bothwell, J., Mylona, P., Miedema, H., Torres, M., Linstead, P., Jones, J., Reactive oxygen species produced by NADPH oxidase regulate plant cell growth (2003) Nature, 422, pp. 442-446 
504 |a Karlsson, M., Kurz, T., Brunk, U., Nilsson, S., Frennesson, C., What does the commonly used DCF test for oxidative stress really show? (2010) Biochem J, 428, pp. 183-190 
520 3 |a Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. All rights are reserved.  |l eng 
593 |a IFIByNE (CONICET), FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina 
690 1 0 |a 2',7-DIHIDRO DICLORO FLUORESCEIN 
690 1 0 |a ROOT HAIR 
690 1 0 |a ROS 
700 1 |a Mangano, S. 
700 1 |a Estevez, J.M. 
773 0 |d Springer New York, 2014  |h pp. 67-71  |p Plant Cell Expans.: Methods and Protoc.  |z 9781493919024  |z 9781493919017  |t Plant Cell Expansion: Methods and Protocols 
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