Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tube...

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Autor principal: Borassi, C.
Otros Autores: Di Giorgio, J.P, Scarpin, M.R, Muschietti, J., Estevez, J.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer New York 2014
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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100 1 |a Borassi, C. 
245 1 0 |a Optimized method for growing in vitro Arabidopsis thaliana pollen tubes 
260 |b Springer New York  |c 2014 
270 1 0 |m Borassi, C.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos AiresArgentina 
506 |2 openaire  |e Política editorial 
504 |a Cheung, A.Y., Hen-ming, W., Structural and signaling networks for the polar cell growth machinery in pollen tubes (2008) Annu Rev Plant Biol, 59, pp. 547-572 
504 |a Cole, R.A., Fowler, J.E., Polarized growth:maintaining focus on the tip (2006) Curr Opin Plant Biol, 9, pp. 579-588 
504 |a Chebli, Y., Geitmann, A., Mechanical principles governing pollen tube growth (2007) Funct Plant Sci Biotechnol, 1, pp. 232-245 
504 |a Boavida, L.C., McCormick, S., Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana (2007) Plant J, 52, pp. 570-582 
520 3 |a Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015. All rights are reserved.  |l eng 
593 |a Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Dr. Héctor Torres (INGEBI-CONICET), Buenos Aires, Argentina 
593 |a Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina 
690 1 0 |a ARABIDOPSIS POLLEN 
690 1 0 |a CELL EXPANSION 
690 1 0 |a POLLEN TUBE GROWTH 
700 1 |a Di Giorgio, J.P. 
700 1 |a Scarpin, M.R. 
700 1 |a Muschietti, J. 
700 1 |a Estevez, J.M. 
773 0 |d Springer New York, 2014  |h pp. 41-47  |p Plant Cell Expans.: Methods and Protoc.  |z 9781493919024  |z 9781493919017  |t Plant Cell Expansion: Methods and Protocols 
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