Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not fo...
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1977
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 06038caa a22008537a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1390 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203045.0 | ||
| 008 | 190411s1977 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0017375249 | |
| 024 | 7 | |2 cas |a adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ABBIA | ||
| 100 | 1 | |a Flawiá, M.M. | |
| 245 | 1 | 0 | |a Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
| 260 | |c 1977 | ||
| 270 | 1 | 0 | |m Torres, H.N. |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Flawiá, Tellez-Iñón, Torres, (1972) Biochemistry of the Glycosidic Linkage, pp. 541-571. , R. Piras, H.G. Pontis, Academic Press, New York | ||
| 504 | |a Flawiá, Torres, (1972) J. Biol. Chem, 247, pp. 6873-6879 | ||
| 504 | |a Flawiá, Torres, (1972) J. Biol. Chem, 247, pp. 6880-6883 | ||
| 504 | |a Flawiá, Torres, (1972) Biochim. Biophys. Acta, 289, pp. 428-432 | ||
| 504 | |a Flawiá, Torres, (1972) Proc. Nat. Acad. Sci. USA, 69, pp. 2870-2873. , 2nd ed | ||
| 504 | |a Flawiá, Torres, Insulin inhibition of membrane-bound adenylate cyclase in Neurospora crassa (1973) FEBS Letters, 30, p. 7478 | ||
| 504 | |a Flawiá, Torres, Biol. Chem (1973) Biol. Chem, 248, pp. 4517-4520 | ||
| 504 | |a Lindegren, A six-point map of the sex-chromosome ofNeurospora crassa (1936) Journal of Genetics, 32, pp. 243-256 | ||
| 504 | |a Perkins, New Markers and Multiple Point Linkage Data in Neurospora. (1959) Genetics, 44, pp. 1185-1208 | ||
| 504 | |a Garnjobst, Tatum, (1970) Genetics, 66, pp. 281-290 | ||
| 504 | |a Terenzi, Flawiá, Torres, (1974) Biochem. Biophys. Res. Commun, 58, pp. 990-996 | ||
| 504 | |a Barrat, Ogata, (1972) Neurospora Newslett, 19, pp. 34-105 | ||
| 504 | |a Emerson, (1963) Genetica, 34, pp. 162-182 | ||
| 504 | |a Mays, (1969) Genetics, 63, pp. 781-794 | ||
| 504 | |a Vogel, (1956) Microbiol. Genet. Bull, 13, p. 4243 | ||
| 504 | |a Westergaard, Mitchell, Neurospora V. A Synthetic Medium Favoring Sexual Reproduction (1947) American Journal of Botany, 34, pp. 573-577 | ||
| 504 | |a Davis, Serres, (1970) Methods in Enzymology, 17, pp. 79-143. , 2nd ed., H. Tabor, C.W. Tabor, Academic Press, New York, Part A | ||
| 504 | |a Phillips, Morrison, (1971) Biochemistry, 10, pp. 1766-1771 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem, 193, pp. 265-275 | ||
| 504 | |a Laemmli, (1970) Nature (London), 227, pp. 680-685 | ||
| 504 | |a Studier, (1973) J. Mol. Biol, 79, pp. 237-248 | ||
| 504 | |a Glossmann, Neville, Jr., (1971) J. Biol. Chem, 246, pp. 6339-6346 | ||
| 504 | |a Fairbanks, Steck, Wallach, (1971) Biochemistry, 10, pp. 2606-2617 | ||
| 504 | |a Fischer, Krebs, (1962) Methods in Enzymology, 5, pp. 369-373. , 2nd ed., S.P. Colowick, N.O. Kaplan, Academic Press, New York | ||
| 504 | |a Terenzi, Flawiá, Tellez-Iñón, Torres, (1976) J. Bacteriol, 126, pp. 91-99 | ||
| 504 | |a Torres, Flawiá, Terenzi, Tellez-Iñón, (1975) Adv. Cyclic Nucleotide Res, 5, pp. 67-78 | ||
| 504 | |a Scott, Solomon, (1975) J. Bacteriol, 122, pp. 454-463 | ||
| 520 | 3 | |a Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the "crisp" morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. © 1977. |l eng | |
| 536 | |a Detalles de la financiación: Jane Coffin Childs Memorial Fund for Medical Research, JCC Fund | ||
| 536 | |a Detalles de la financiación: The authors are grateful to Drs. Luis F. Leloir and Pedro Cuatrecasas for helpful discussions and criticism. This work was supported in part by grants from the Jane Coffin Childs Memorial Foundation for Medical Research and Consejo National de In-vestigaciones Cientificas y Tecnicas (Republica Argentina). The authors are Career Investigators of the latter institution. H.N.T. is a Fellow of the John Simon Guggenheim Memorial Foundation. | ||
| 593 | |a Instituto de Investigaciones Bioquímicas Fundación Campomar and Facultad de Ciencias Exactas y Naturales, Buenos Aires (28), Argentina | ||
| 593 | |a Department of Molecular Biology, The Wellcome Research Laboratories, Burroughs Wellcome Company, 3030 Cornwallis Road, Research Triangle Park, NC 27709, United States | ||
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a ENZYME DEFICIENCY |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a MICROORGANISM |
| 690 | 1 | 0 | |a MUTANT |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a THEORETICAL STUDY |
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a CROSSES, GENETIC |
| 690 | 1 | 0 | |a MEMBRANES |
| 690 | 1 | 0 | |a MUTATION |
| 690 | 1 | 0 | |a NEUROSPORA |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 700 | 1 | |a Terenzi, H.F. | |
| 700 | 1 | |a Torres, H.N. | |
| 773 | 0 | |d 1977 |g v. 180 |h pp. 334-342 |k n. 2 |p Arch. Biochem. Biophys. |x 00039861 |w (AR-BaUEN)CENRE-1377 |t Archives of Biochemistry and Biophysics | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0003-9861(77)90046-7 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p334_Flawia |y Registro en la Biblioteca Digital |
| 961 | |a paper_00039861_v180_n2_p334_Flawia |b paper |c PE | ||
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| 963 | |a VARI | ||
| 999 | |c 62343 | ||