A phasin with extra talents: A polyhydroxyalkanoate granule-associated protein has chaperone activity
Phasins are proteins associated to intracellular polyhydroxyalkanoate granules that affect polymer accumulation and the number and size of the granules. Previous work demonstrated that a phasin from Azotobacter sp FA-8 (PhaP<inf>Az</inf>) had an unexpected growth-promoting and stress-pro...
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Blackwell Publishing Ltd
2015
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 14446caa a22011777a 4500 | ||
|---|---|---|---|
| 001 | PAPER-13751 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204410.0 | ||
| 008 | 190411s2015 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84928298441 | |
| 024 | 7 | |2 cas |a Bacterial Proteins; Molecular Chaperones; phasin; Plant Lectins; Polyhydroxyalkanoates | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ENMIF | ||
| 100 | 1 | |a Mezzina, M.P. | |
| 245 | 1 | 2 | |a A phasin with extra talents: A polyhydroxyalkanoate granule-associated protein has chaperone activity |
| 260 | |b Blackwell Publishing Ltd |c 2015 | ||
| 270 | 1 | 0 | |m Pettinari, M.J.; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICETArgentina |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Phasins are proteins associated to intracellular polyhydroxyalkanoate granules that affect polymer accumulation and the number and size of the granules. Previous work demonstrated that a phasin from Azotobacter sp FA-8 (PhaP<inf>Az</inf>) had an unexpected growth-promoting and stress-protecting effect in Escherichia coli, suggesting it could have chaperone-like activities. In this work, in vitro and in vivo experiments were performed in order to investigate this possibility. PhaP<inf>Az</inf> was shown to prevent in vitro thermal aggregation of the model protein citrate synthase and to facilitate the refolding process of this enzyme after chemical denaturation. Microscopy techniques were used to analyse the subcellular localization of PhaP<inf>Az</inf> in E.coli strains and to study the role of PhaP<inf>Az</inf> in in vivo protein folding and aggregation. PhaP<inf>Az</inf> was shown to colocalize with inclusion bodies of PD, a protein that aggregates when overexpressed. A reduction in the number of inclusion bodies of PD was observed when it was coexpressed with PhaP<inf>Az</inf> or with the known chaperone GroELS. These results demonstrate that PhaP<inf>Az</inf> has chaperone-like functions both in vitro and in vivo in E.coli recombinants, and suggests that phasins could have a general protective role in natural polyhydroxyalkanoate producers. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. |l eng | |
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina | ||
| 593 | |a Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain | ||
| 690 | 1 | 0 | |a AZOTOBACTER |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a BACTERIAL PROTEIN |
| 690 | 1 | 0 | |a CHAPERONE |
| 690 | 1 | 0 | |a PHASIN |
| 690 | 1 | 0 | |a PLANT LECTIN |
| 690 | 1 | 0 | |a POLYHYDROXYALKANOIC ACID |
| 690 | 1 | 0 | |a AZOTOBACTER |
| 690 | 1 | 0 | |a CELL INCLUSION |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a GENETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PROTEIN FOLDING |
| 690 | 1 | 0 | |a AZOTOBACTER |
| 690 | 1 | 0 | |a BACTERIAL PROTEINS |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a INCLUSION BODIES |
| 690 | 1 | 0 | |a MOLECULAR CHAPERONES |
| 690 | 1 | 0 | |a PLANT LECTINS |
| 690 | 1 | 0 | |a POLYHYDROXYALKANOATES |
| 690 | 1 | 0 | |a PROTEIN FOLDING |
| 700 | 1 | |a Wetzler, D.E. | |
| 700 | 1 | |a de Almeida, A. | |
| 700 | 1 | |a Dinjaski, N. | |
| 700 | 1 | |a Prieto, M.A. | |
| 700 | 1 | |a Pettinari, M.J. | |
| 773 | 0 | |d Blackwell Publishing Ltd, 2015 |g v. 17 |h pp. 1765-1776 |k n. 5 |p Environ. Microbiol. |x 14622912 |w (AR-BaUEN)CENRE-4624 |t Environmental Microbiology | |
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