New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
Sphingolipids are involved in a wide range of physiological and pathological processes none only as signaling molecules but also as key structural components regulating the lateral organization of cellular membranes. The preferential interaction of these biomolecules with cholesterol support the act...
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Nova Science Publishers, Inc.
2015
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| LEADER | 20966caa a22013097a 4500 | ||
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| 001 | PAPER-13652 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204402.0 | ||
| 008 | 190411s2015 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84958686372 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Traian, M.M.D. | |
| 245 | 1 | 0 | |a New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes |
| 260 | |b Nova Science Publishers, Inc. |c 2015 | ||
| 270 | 1 | 0 | |m Sánchez, S.A.; Departamento de Polímeros, Facultad de Ciencias Químicas, Universidad de Concepción, Edmundo Larenas 129, Chile; email: susanchez@udec.cl |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Sphingolipids are involved in a wide range of physiological and pathological processes none only as signaling molecules but also as key structural components regulating the lateral organization of cellular membranes. The preferential interaction of these biomolecules with cholesterol support the actual theory related with membrane heterogeneity in vivo, the raft theory. Rafts are believed to be highlydynamic and small domains enriched in sphingolipids, cholesterol and certain proteins present in the membrane of cells. The idea of these domains compartmentalizing cellular processes is a central hypothesis in biomedical research from immunology, virology, neurobiology to cancer. The use of microscopy to study lateral heterogeneity in biological membranes was developed during the nineties with the use of artificial models systems such as giant unillamelar vesicles and supported-lipid bilayers. The combination of confocal and two-photon microscopy techniques with fluorescent and solvatochromic probes like Laurdan enabled the acquisition of spatially-resolved information about the fluidity and/or order of artificial bilayers showing phase segregation. The development of new techniques combining Laurdan imaging with fluorescence fluctuation spectroscopy allowed the detection of highlypacked microdomains in natural cell membranes. In this article we review these exciting new approaches that open a window to further characterize these sphingolipid-enriched domains in cell membranes during both physiological and pathological processes. © 2015 by Nova Science Publishers, Inc. All rights reserved. |l eng | |
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Polímeros, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile | ||
| 700 | 1 | |a Sánchez, S.A. | |
| 700 | 1 | |a Levi, V. | |
| 773 | 0 | |d Nova Science Publishers, Inc., 2015 |h pp. 1-19 |p Sphingomyelin and Ceramides: Occur., Biosynth. and Role in Dis. |z 9781634825856 |z 9781634825535 |t Sphingomyelin and Ceramides: Occurrence, Biosynthesis and Role in Disease | |
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