Estrogen receptor in rat pancreatic islets
The cytosol fraction of pancreatic islets of the female rat was found to contain a specifically binding protein for [3H]-estradiol. This protein was heat sensitive and the [3H]-estradiol binding was eliminated by treatment with protease and sulphydryl-blocking agents. Scatchard analysis of the cytos...
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| Lenguaje: | Inglés |
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1979
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 07368caa a22009377a 4500 | ||
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| 001 | PAPER-1354 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203042.0 | ||
| 008 | 190411s1979 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0018512235 | |
| 024 | 7 | |2 cas |a estradiol, 50-28-2; Estradiol, 50-28-2; Receptors, Estrogen | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JSTBB | ||
| 100 | 1 | |a Tesone, M. | |
| 245 | 1 | 0 | |a Estrogen receptor in rat pancreatic islets |
| 260 | |c 1979 | ||
| 270 | 1 | 0 | |m Tesone, M. |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Lewis, Foglia, Rodrǐguez, The effects of steroids on the incidence of diabetes in rats after subtotal pancreatectomy (1950) Endocrinology, 46, pp. 111-121 | ||
| 504 | |a Houssay, Foglia, Rodrǐguez, Acta endocr., Copenh. (1954) Production or prevention of some types of experimental diabetes by oestrogens or corticosteroids, 17, pp. 146-166 | ||
| 504 | |a Costrini, Kalkhoff, Relative effects of pregnancy, estradiol and progesterone on plasma insulin and pancreatic islet insulin secretion (1971) J. clin. Invest., 50, pp. 992-999 | ||
| 504 | |a Howell, Tyhurst, Green, Direct effects of progesterone on rat islets of Langerhans invivo and in tissue culture (1977) Diabetologia, 13, pp. 579-583 | ||
| 504 | |a Hellman, The islets of Langerhans in the rat during pregnancy and lactation, with special reference to the changes in the B/A cell ratio (1960) Acta Obstet. Gynecol. Scand., 39, pp. 331-337 | ||
| 504 | |a Malaisse, Hormonal and environmental modification of islet activity (1972) Handbook of Physiology, 1, pp. 237-260. , R.O. Greep, E.B. Astwood, American Physiological Society, Washington, Sec. 7 | ||
| 504 | |a Kalkhoff, Richardson, Beck, Relative effects of pregnancy, human placental lactogen, and prednisolone on carbohydrate tolerance in normal and subclinical diabetic subjects (1969) Diabetes, 18, pp. 153-163 | ||
| 504 | |a Jensen, DeSombre, Mechanism of action of the female sex hormones (1972) Ann. Rev. Biochem., 41, pp. 203-230 | ||
| 504 | |a Jensen, DeSombre, Estrogen-receptor interaction (1973) Science, N.Y., 182, pp. 126-134 | ||
| 504 | |a Liao, Cellular receptors and mechanisms of action of steroid hormones (1975) Int. Rev. Cytol., 40, pp. 87-172 | ||
| 504 | |a Charreau, Baldia, Binding of estradiol receptor complexes to isolated human breast chromatin (1977) Mol. Cell. Biochem., 16, pp. 79-86 | ||
| 504 | |a Stumpf, Nuclear concentration of 3H-estradiol in target tissues. Dry-mount autoradiography of vagina, oviduct, ovary, testis, mammary tumor, liver and adrenal (1969) Endocrinology, 85, pp. 31-37 | ||
| 504 | |a Sandberg, Kirdani, Varkarakis, Murphy, Estrogen receptor protein of pancreas (1973) Steroids, 22, pp. 259-271 | ||
| 504 | |a Andersson, Hellerström, Metabolic characteristics of isolated pancreatic islets in tissue culture (1972) Diabetes, 21, pp. 546-554 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Korenman, Dukes, Specific estrogen binding by the cytoplasma of human breast carcinoma (1970) J. clin. Endocr., 30, pp. 639-645 | ||
| 504 | |a Scatchard, The attraction of proteins for small molecules and ions (1949) Annals of the New York Academy of Sciences, 51, pp. 660-672 | ||
| 504 | |a Reichman, Villee, Estradiol binding by rat thymus cytosol (1978) J. steroid Biochem., 9, pp. 637-641 | ||
| 504 | |a Sanborn, Rao, Korenman, Interaction of 17β-estradiol and its specific uterine receptor. Evidence for complex kinetic and equilibrium behavior (1971) Biochemistry, 10, pp. 4955-4961 | ||
| 504 | |a Anderson, Clark, Peck, Jr., Oestrogen and nuclear binding sites. Determination of specific sites by [3H]-oestradiol exchange (1972) Biochem. J., 126, pp. 561-567 | ||
| 504 | |a Beck, Progestin enhancement of the plasma insulin response to glucose in rhesus monkeys (1969) Diabetes, 18, pp. 145-152 | ||
| 504 | |a Kalkhoff, Jacobson, Lemper, Progesterone, pregnancy and the augmented plasma insulin response (1970) J. clin. Endocr. Metab., 31, pp. 24-28 | ||
| 520 | 3 | |a The cytosol fraction of pancreatic islets of the female rat was found to contain a specifically binding protein for [3H]-estradiol. This protein was heat sensitive and the [3H]-estradiol binding was eliminated by treatment with protease and sulphydryl-blocking agents. Scatchard analysis of the cytosol binding reaction, measured by charcoal-dextran assay, indicated a single class of estradiol-binding sites having high affinity (Kd = 2.9 × 10-8 M at 0°C). The number of binding sites was calculated to be 29.6 fmol/mg cytosol protein in whole pancreas and 170 fmol/mg cytosol protein in isolated pancreatic islets after collagenase treatment. Competition studies indicated high specificity for the binding reaction, since excess (100-fold) unlabelled estrogens, diethylstilbestrol and the antiestrogen nafoxidine, all significantly reduced the binding of [3H]-estradiol. On the other hand, the nonestrogenic steroids dihydrotestosterone, corticosterone and progesterone had no significant effects on [3H]-estradiol binding. The complex had a sedimentation coefficient of 4-5 S in sucrose density gradient centrifugation in low salt. In streptozotocin-diabetic and in 3-wk pregnant rats a significant decrease in the binding of estradiol to pancreas islet cytosol was found. © 1979. |l eng | |
| 593 | |a Laboratorio de Esteroides, Instituto de Biologǐa y Medicina Experimental, Obligado 2490, (1428) Buenos AiresArgentina | ||
| 593 | |a Departamento de Quǐmica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a ESTROGEN RECEPTOR |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BINDING COMPETITION |
| 690 | 1 | 0 | |a CASTRATION |
| 690 | 1 | 0 | |a CYTOSOL |
| 690 | 1 | 0 | |a EXPERIMENTAL DIABETES MELLITUS |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PANCREAS ISLET |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BINDING, COMPETITIVE |
| 690 | 1 | 0 | |a CASTRATION |
| 690 | 1 | 0 | |a CYTOSOL |
| 690 | 1 | 0 | |a DIABETES MELLITUS, EXPERIMENTAL |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a ISLETS OF LANGERHANS |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RECEPTORS, ESTROGEN |
| 700 | 1 | |a Chazenbalk, G.D. | |
| 700 | 1 | |a Ballejos, G. | |
| 700 | 1 | |a Charreau, E.H. | |
| 773 | 0 | |d 1979 |g v. 11 |h pp. 1309-1314 |k n. 3 |p J. Steroid Biochem. |x 00224731 |t Journal of Steroid Biochemistry | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018512235&doi=10.1016%2f0022-4731%2879%2990201-2&partnerID=40&md5=0a15b14f8a47e353615970e891254598 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0022-4731(79)90201-2 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00224731_v11_n3_p1309_Tesone |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v11_n3_p1309_Tesone |y Registro en la Biblioteca Digital |
| 961 | |a paper_00224731_v11_n3_p1309_Tesone |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 62307 | ||