Androgen receptors in the diabetic rat

Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein....

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Autor principal: Tesone, M.
Otros Autores: Oliveira-Filho, R.M, Biella de Souza Valle, L., Calvo, J.C, Barañao, J.L.S, Foglia, V.G, Charreau, E.H
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer-Verlag 1980
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Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
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024 7 |2 scopus  |a 2-s2.0-0018889422 
024 7 |2 cas  |a androstanolone, 521-18-6; insulin, 9004-10-8; metribolone, 965-93-5; streptozocin, 18883-66-4; testosterone, 58-22-0; Estrenes; Insulin, Long-Acting; Metribolone, 965-93-5; Receptors, Androgen; Receptors, Steroid; Testosterone, 58-22-0; Testosterone Congeners 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a DBTGA 
100 1 |a Tesone, M. 
245 1 0 |a Androgen receptors in the diabetic rat 
260 |b Springer-Verlag  |c 1980 
270 1 0 |m Tesone, M.; Laboratorio de Esteroides, Instituto de Biología y Medicina ExperimentalArgentina 
506 |2 openaire  |e Política editorial 
504 |a Foglia, V.G., Borghelli, R.F., Chieri, R.A., Fernández Collazo, E.L., Spindler, L., Wesely, O., Sexual disturbances in the diabetic rat (1963) Diabetes, 12, pp. 231-237 
504 |a Schöffling, K., Federlin, K., Ditschuneit, H., Pfeiffer, E.F., Disorders of sexual function in male diabetics (1963) Diabetes, 12, pp. 519-527 
504 |a Schöffling, K., Federlin, K., Schmitt, W., Pfeiffer, E.F., Histometric investigations on the testicular tissue of rats with alloxan diabetes and Chinese hamsters with spontaneous diabetes (1967) Acta Endocrinol (Kbh), 54, pp. 335-346 
504 |a Foglia, V.G., El peso de los órganos de la rata diabética (1945) Rev Soc Argent Biol, 21, pp. 45-53 
504 |a Foglia, V.G., Rosner, J.M., de Cattaneo, Lema, B.E., Sexual disturbances in the male diabetic rat (1969) Horm Metab Res, 1, pp. 72-77 
504 |a de Cattaneo, Irusta, O., Foglia, V.G., Acción de las gonadotrofinas e insulina sobre la producción de testosterona por testículos de ratas diabéticas (1973) Rev Soc Argent Biol, 49, pp. 56-60 
504 |a Tesone, M., Valle, L.B.S., Foglia, V.G., Charreau, E.H., Endocrine function of the testis in streptozotocin diabetic rats (1976) Acta Physiol Lat, 26, pp. 387-394 
504 |a Angervall, L., Hesselsjo, R., Nilsson, S., Tisell, L.E., Action of testosterone on ventral prostate, dorsolateral prostate, coagulating glands and seminal vesicles of castrated alloxan-diabetic rats (1967) Diabetologia, 3, pp. 395-401 
504 |a Oksanen, A., Tuohimaa, P., Testicular lesions of streptozotocin diabetic rats (1975) Horm Res, 6, pp. 138-144 
504 |a Calame, S.S., Lostroh, A.J., Effect of insulin and lack of effect of testosterone on the protein of ventral prostates from castrated mice maintained as organ cultures (1964) Endocrinology, 75, pp. 451-454 
504 |a Lostroh, A.J., Effect of testosterone and insulin in vitro on maintenance and repair of the secretory epithelium of the mouse prostate (1971) Endocrinology, 88, pp. 500-503 
504 |a Sufrin, G., Prutkin, L., Experimental diabetes and the response of the sex accessory organs of the castrated male rat to testosterone propionate. Biochemical and electron microscopic observations (1974) Invest Urol, 11, pp. 361-369 
504 |a Bonne, C., Raynaud, J.P., Assay of androgen binding sites by exchange with methyltrienolone (R1881) (1976) Steroids, 27, pp. 497-507 
504 |a Katzenellenbogen, J.A., Johnson, H.S., Carlson, K.E., Studies on the uterine cytoplasmic estrogen binding protein. Thermal stability and ligand dissociation rate. An assay of empty and filled sites by exchange (1973) Biochemistry, 12, pp. 4092-4099 
504 |a Tindall, D.J., Hansson, V., McLean, W.S., Ritzen, E.M., Nayfeh, S.N., French, F.S., Androgen-binding proteins in rat epididymis: properties of a cytoplasmic receptor for androgen similar to the androgen receptor in ventral prostate and different from androgen-binding protein (ABP) (1975) Mol Cell Endocrinol, 3, pp. 83-101 
504 |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 
504 |a Scatchard, G., The attraction of proteins for small molecules and ions (1949) Annals of the New York Academy of Sciences, 51, pp. 660-672 
504 |a Hunt, E.L., Bailey, D.W., The effects of alloxan diabetes on the reproductive system of young male rats (1961) Acta Endocrinol (Kbh), 38, pp. 432-440 
504 |a Howland, B.E., Zebrowski, E.J., Serum and pituitary gonadotropin levels in alloxan-diabetic rats (1974) Horm Metab Res, 6, pp. 121-124 
504 |a Jung, L., Baulieu, E.E., Neo-nuclear androgen receptor in rat ventral prostate (1971) Biochimie, 53, pp. 807-817 
504 |a Mainwaring, W.I.P., Mangan, F.R., A study of the androgen receptors in a variety of androgen-sensitive tissues (1973) J Endocrinol, 59, pp. 121-139 
504 |a Sullivan, J.N., Strott, C.A., Evidence for an androgen-independent mechanism regulating the levels of receptor in target tissue (1973) J Biol Chem, 248, pp. 3202-3208 
504 |a Bruchovsky, N., Cravan, S., Prostatic involution: effect on androgen receptors and intracellular androgen transport (1974) Biochem Biophys Res Commun, 62, pp. 837-843 
504 |a Blondeau, J.P., Corpéchot, C., Goascogne, C., Baulieu, E.E., Robel, P., Androgen receptors in the rat ventral prostate and their hormonal control (1975) Vitam Horm, 33, pp. 319-345 
504 |a Howland, B.E., Zebrowsky, E.J., Some effects of experimentally-induced diabetes on pituitary-testicular relationships in rats (1976) Horm Metab Res, 8, pp. 465-469 
504 |a Charreau, E.H., Calvo, J.C., Tesone, M., Valle, L.B.S., Barañao, J.L., Insulin regulation of Leydig cell luteinizing hormone receptors (1978) J Biol Chem, 253, pp. 2504-2506 
520 3 |a Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein. In comparison with normal (N) rats, the replacement therapy of diabetic (D) animals with insulin (D+I) or testosterone (D+T) was able to restore epididymal weight (N = 0.40 ±0.04 g; D = 0.18 ± 0.02 g; D+I = 0.42 ± 0.05 g; D+T = 0.40 ± 0.06 g) and total prostate weight (N = 0.24 ± 0.02 g; D = 0.15 ± 0.02 g; D+I = 0.24 ± 0.05 g; D+T = 0.35 ± 0.06 g). Testicular endogenous content of testosterone was restored after insulin treatment (N = 154 ± 13 ng/testis; D = 41 ± 5 ng/testis; D+I = 142 ± 9 ng/testis), and significant improvements of serum testosterone levels were also achieved (N = 540 ± 64 ng/100 ml; D = 238 ± 37 ng/100 ml; D+I = 358 ± 18 ng/100 ml). Prostate cytosol of streptozotocin-diabetic rats had strongly lowered capacity for 3H-R1881 binding compared with controls (94 and 12 fmol/mg protein, respectively). Testosterone treatment produced a 3.3-fold improvement of this lowered value, whereas the increment seen with insulin was less (1.5-fold). It is emphasized that some of the improvements caused by insulin replacement therapy in diabetic animals are due to the partial restoration of testosterone secretion. Thus, the combined actions of insulin and testosterone (instead of insulin alone) seem to be of major importance in the maintenance and regulation of accessory sex glands function. © 1980 Springer-Verlag.  |l eng 
593 |a Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, Argentina 
593 |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
690 1 0 |a ANDROGEN RECEPTORS 
690 1 0 |a CYTOSOL BINDING SITES 
690 1 0 |a R1881 
690 1 0 |a RAT PROSTATE 
690 1 0 |a STREPTOZOTOCIN-DIABETES 
690 1 0 |a ANDROGEN RECEPTOR 
690 1 0 |a ANDROSTANOLONE 
690 1 0 |a ANDROSTANOLONE H 3 
690 1 0 |a DRUG RECEPTOR 
690 1 0 |a INSULIN 
690 1 0 |a METHYLTRIENOLONE H 3 
690 1 0 |a METRIBOLONE 
690 1 0 |a RADIOISOTOPE 
690 1 0 |a STREPTOZOCIN 
690 1 0 |a TESTOSTERONE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ARTICLE 
690 1 0 |a DIABETES MELLITUS 
690 1 0 |a ENDOCRINE SYSTEM 
690 1 0 |a MALE GENITAL SYSTEM 
690 1 0 |a PROSTATE 
690 1 0 |a RAT 
690 1 0 |a ANIMALS 
690 1 0 |a BODY WEIGHT 
690 1 0 |a CYTOSOL 
690 1 0 |a DIABETES MELLITUS, EXPERIMENTAL 
690 1 0 |a EPIDIDYMIS 
690 1 0 |a ESTRENES 
690 1 0 |a INSULIN, LONG-ACTING 
690 1 0 |a MALE 
690 1 0 |a METRIBOLONE 
690 1 0 |a ORGAN SIZE 
690 1 0 |a PROSTATE 
690 1 0 |a RATS 
690 1 0 |a RECEPTORS, ANDROGEN 
690 1 0 |a RECEPTORS, STEROID 
690 1 0 |a TESTIS 
690 1 0 |a TESTOSTERONE 
690 1 0 |a TESTOSTERONE CONGENERS 
653 0 0 |a r 1881 
700 1 |a Oliveira-Filho, R.M. 
700 1 |a Biella de Souza Valle, L. 
700 1 |a Calvo, J.C. 
700 1 |a Barañao, J.L.S. 
700 1 |a Foglia, V.G. 
700 1 |a Charreau, E.H. 
773 0 |d Springer-Verlag, 1980  |g v. 18  |h pp. 385-390  |k n. 5  |p Diabetologia  |x 0012186X  |t Diabetologia 
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