Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations

Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained bindin...

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Autor principal: Kornblihtt, A.R
Otros Autores: Flawiá, M.M, de Mendoza, D., Glikin, G., Farías, R., Torres, H.N
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Martinus Nijhoff/Dr. W. Junk Publishers 1981
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 cas  |a adenylate cyclase, 9012-42-4; liothyronine, 6138-47-2, 6893-02-3; Adenylate Cyclase, 4.6.1.1; Triiodothyronine, 6893-02-3 
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100 1 |a Kornblihtt, A.R. 
245 1 0 |a Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations 
260 |b Martinus Nijhoff/Dr. W. Junk Publishers  |c 1981 
270 1 0 |m Kornblihtt, A.R.; Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Fac. de Ciencias Exactas y Naturales, Universidad Nacional de Tucumán, Obligado 2490, Buenos Aires, 1428, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Limbrid, L.E., Lefkowitz, R.J., (1977) J. Biol. Chem., 252, pp. 799-802 
504 |a Haga, T., Haga, K., Gilman, A.G., (1977) J. Biol. Chem., 252, pp. 5776-5782 
504 |a Welton, A.F., Lad, P.M., Newby, A.C., Yamamura, N., Nicosia, S., Rodbell, M., (1977) J. Biol. Chem., 252, pp. 5947-5950 
504 |a Braun, T., Dods, R.F., (1975) Proc. Natl. Acad. Sci. U.S.A., 72, pp. 1097-1101 
504 |a Neer, E.J., (1978) J. Biol. Chem., 253, pp. 5808-5812 
504 |a Flawia, M. M., Kornblihtt, A., de Mendoza, D., & Torres, H. N., 1980. Mol. and Cell. Biochem; Lantham, K.R., Ring, J.C., Baxter, J.D., (1976) J. Biol. Chem., 251, pp. 7388-7397 
504 |a Worthington Enzyme Manual 1972. Worthington Biochemical Corporation: pp. 7–12, Freehold; Terenzi, H.F., Roselino, E., Passeron, S., (1971) Eur. J. Biochem., 18, pp. 342-350 
520 3 |a Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the lowest binding capacity. © 1981 Martinus Nijhoff/Dr W. Junk Publishers.  |l eng 
593 |a Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Fac. de Ciencias Exactas y Naturales, Universidad Nacional de Tucumán, Obligado 2490, Buenos Aires, 1428, Argentina 
593 |a Instituto de Química Biológica, Universidad Nacional de Tucumán, Obligado 2490, Buenos Aires, 1428, Argentina 
690 1 0 |a ADENYLATE CYCLASE 
690 1 0 |a LIOTHYRONINE 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a MALE GENITAL SYSTEM 
690 1 0 |a RAT 
690 1 0 |a TESTIS 
690 1 0 |a ADENYLATE CYCLASE 
690 1 0 |a ANIMALS 
690 1 0 |a CYTOSOL 
690 1 0 |a KINETICS 
690 1 0 |a MALE 
690 1 0 |a PROTEIN BINDING 
690 1 0 |a RATS 
690 1 0 |a SEMINIFEROUS TUBULES 
690 1 0 |a TESTIS 
690 1 0 |a TRIIODOTHYRONINE 
690 1 0 |a ANIMALIA 
700 1 |a Flawiá, M.M. 
700 1 |a de Mendoza, D. 
700 1 |a Glikin, G. 
700 1 |a Farías, R. 
700 1 |a Torres, H.N. 
773 0 |d Martinus Nijhoff/Dr. W. Junk Publishers, 1981  |g v. 36  |h pp. 23-27  |k n. 1  |p Mol Cell Biochem  |x 03008177  |w (AR-BaUEN)CENRE-3498  |t Molecular and Cellular Biochemistry 
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