Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase
Particulate cell fractions of mycelium of Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 0.5 m KCl. Homogenates prepared in the presence of Lubrol PX or KCl exhibit two- to threefold more activity compared to homogenates prepared in the absence of detergent or...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1982
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 07192caa a22009977a 4500 | ||
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| 001 | PAPER-1249 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203036.0 | ||
| 008 | 190411s1982 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0020209701 | |
| 024 | 7 | |2 cas |a adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ABBIA | ||
| 100 | 1 | |a Cantore, M.L. | |
| 245 | 1 | 0 | |a Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase |
| 260 | |c 1982 | ||
| 270 | 1 | 0 | |m Passeron, S.; Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a De Haën, Adenylate cyclase. A new kinetic analysis of the effects of hormones and fluoride ion. (1974) J Biol Chem, 249, pp. 2756-2762 | ||
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| 520 | 3 | |a Particulate cell fractions of mycelium of Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 0.5 m KCl. Homogenates prepared in the presence of Lubrol PX or KCl exhibit two- to threefold more activity compared to homogenates prepared in the absence of detergent or salt. The membrane-bound enzyme prepared in the presence of 2% Lubrol PX was studied in detail. The kinetic properties of this adenylate cyclase were as expected for an allosteric enzyme with the ATP · Mn2- complex as substrate and free Mn2+ ions as activator. Unchelated ATP inhibits the reaction by competing with the substrate. The KCl-solubilized enzyme was partially purified by ion-exchange chromatography, ammonium sulfate precipitation, and gel filtration. Molecular parameters of the solubilized enzyme are: Stokes radius, 8.6 nm; S20,w, 13S; Mr 510,000; and frictional ratio, 1.60. © 1982. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: i This investigation was supported the Consejo National de Investigaciones y TQcnicas (CONICET), Argentina, Estado de Ciencia y Tecnologia Comision National de Energia * S.P. is a Career Investigator a To whom correspondence Laboratorio de Biolbgia Quimica Biologica. 4 Abbreviations phosphate; anesulfonic | ||
| 593 | |a Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL FRACTIONATION |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MUCOR |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MUCOR |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a SUBCELLULAR FRACTIONS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 700 | 1 | |a Passeron, S. | |
| 773 | 0 | |d 1982 |g v. 219 |h pp. 1-11 |k n. 1 |p Arch. Biochem. Biophys. |x 00039861 |w (AR-BaUEN)CENRE-1377 |t Archives of Biochemistry and Biophysics | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020209701&doi=10.1016%2f0003-9861%2882%2990127-8&partnerID=40&md5=39d8477211631f3c575c389feb2539b3 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0003-9861(82)90127-8 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00039861_v219_n1_p1_Cantore |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v219_n1_p1_Cantore |y Registro en la Biblioteca Digital |
| 961 | |a paper_00039861_v219_n1_p1_Cantore |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 62202 | ||