A specific method for the quantitative determination of β-glucose-1-phosphate

A specific spectrophotometric determination of β-glucose-1-phosphate has been devised. It allows β-glucose-1-phosphate to be measured in the presence of α-glucose-1-phosphate and of a one hundred-fold excess of glucose-6-phosphate. Phosphoglucomutase for β-glucose-1-phosphate obtained from cells of...

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Autor principal: Belocopitow, E.
Otros Autores: Maréchal, L.R
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1973
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0015838766 
024 7 |2 cas  |a glucose 1 phosphate, 59-56-3; phosphoglucomutase, 9001-81-4; Arsenic, 7440-38-2; Borohydrides; Glucosephosphates; Phosphoglucomutase, EC 5.4.2.2; Protamines; Sulfuric Acids 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a ANBCA 
100 1 |a Belocopitow, E. 
245 1 2 |a A specific method for the quantitative determination of β-glucose-1-phosphate 
260 |c 1973 
270 1 0 |m Belocopitow, E.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado, 2490 Buenos Aires 28, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Maréchal, Belocopitow, (1972) J. Biol. Chem, 247, p. 3223 
504 |a Ben-Zvi, Schramm, (1961) J. Biol. Chem, 236, p. 2186 
504 |a Reithel, (1945) J. Amer. Chem. Soc, 67, p. 1056 
504 |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem, 193, p. 265 
504 |a Somogyi, (1945) J. Biol. Chem, 160, p. 61 
504 |a Nelson, (1944) J. Biol. Chem, 153, p. 375 
504 |a Belocopitow, Maréchal, (1972) Biochemistry of the Glycosidic Linkage, p. 297. , R. Piras, H.G. Pontis, Academic Press, New York 
504 |a Leloir, Cardini, Cabib, (1952) Anal. Asoc. Quím. Arg, 40, p. 228 
520 3 |a A specific spectrophotometric determination of β-glucose-1-phosphate has been devised. It allows β-glucose-1-phosphate to be measured in the presence of α-glucose-1-phosphate and of a one hundred-fold excess of glucose-6-phosphate. Phosphoglucomutase for β-glucose-1-phosphate obtained from cells of Euglena gracilis var. bacillaris must be prepared for the assay. © 1973.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: We gratefully acknowledge the financial support of the Instituto National de la Actividad Hipica, Banco Municipal de la Ciudad de Buenos Aires, and Fondo Especial para la Investigation Cientifica de la Universidad de Buenos Aires. We are also grateful to Dr. Luis F. Leloir for his constant support. and encouragement, to the members of the Instituto de Investigaciones Bioquimicas for consultation and criticisms. and to Miss Margarita Mazzardi for her competent assistance, 
593 |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado, 2490 Buenos Aires 28, Argentina 
690 1 0 |a GLUCOSE 1 PHOSPHATE 
690 1 0 |a PHOSPHOGLUCOMUTASE 
690 1 0 |a METHODOLOGY 
690 1 0 |a ANIMAL 
690 1 0 |a ARSENIC 
690 1 0 |a BOROHYDRIDES 
690 1 0 |a ENZYME ACTIVATION 
690 1 0 |a EUGLENA GRACILIS 
690 1 0 |a GLUCOSEPHOSPHATES 
690 1 0 |a HYDROGEN-ION CONCENTRATION 
690 1 0 |a KINETICS 
690 1 0 |a METHODS 
690 1 0 |a PHOSPHOGLUCOMUTASE 
690 1 0 |a PRECIPITATION 
690 1 0 |a PROTAMINES 
690 1 0 |a STEREOISOMERISM 
690 1 0 |a SULFURIC ACIDS 
700 1 |a Maréchal, L.R. 
773 0 |d 1973  |g v. 53  |h pp. 108-114  |k n. 1  |p Anal. Biochem.  |x 00032697  |w (AR-BaUEN)CENRE-163  |t Analytical Biochemistry 
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856 4 0 |u https://doi.org/10.1016/0003-2697(73)90411-9  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_00032697_v53_n1_p108_Belocopitow  |y Handle 
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