Glycosyl transfer to an acceptor lipid from insects

Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were eq...

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Autor principal: Quesada Allué, L.A
Otros Autores: Belocopitow, E., Maréchal, L.R
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1975
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0016758687 
024 7 |2 cas  |a lipid, 66455-18-3; uridine diphosphate glucose, 133-89-1; uridine diphosphate n acetylglucosamine, 528-04-1; Acetylglucosamine, 7512-17-6; Glucose, 50-99-7; Glycolipids; Phospholipids 
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100 1 |a Quesada Allué, L.A. 
245 1 0 |a Glycosyl transfer to an acceptor lipid from insects 
260 |c 1975 
270 1 0 |m Quesada Allué, L.A.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina 
506 |2 openaire  |e Política editorial 
504 |a Lennarz, (1975) Science, 188, pp. 986-991 
504 |a Behrens, Leloir, (1970) Proc. Natl. Acad. Sci. U. S, 66, pp. 153-159 
504 |a Behrens, Parodi, Leloir, Krisman, (1971) Arch. Biochem. Biophys, 143, pp. 375-383 
504 |a Quesada, Belocopitow, Maréchal, (1974) Communication in the X Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica 
504 |a Wright, Robbins, (1965) Biochim. Biophys. Acta, 104, pp. 594-596 
504 |a Folch, Lees, Sloane Stanley, (1957) J. Biol. Chem, 226, pp. 497-509 
504 |a Krisman, (1972) Biochem. Biophys. Res. Commun, 46, pp. 1206-1212 
504 |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem, 193, pp. 265-275 
504 |a Dankert, Behrens, (1971) Anales de la Sociedad Cientifica Argentina, pp. 145-164. , Número Especial 
504 |a Barr, Hemming, (1971) Biochem. J, 126, pp. 1203-1208 
504 |a Pont Lezica, R., Brett, C., Romero, P. and Dankert, M., submitted for publication; Lahav, Chiu, Lennarz, (1969) J. Biol. Chem, 244, pp. 5890-5898 
504 |a Higashi, Strominger, Sweely, Biosynthesis of the peptidoglycan of bacterial cell walls. XXI. Isolation of free C55-isoprenoid alcohol and of lipid intermediates in peptidoglycan synthesis from Staphylococcus aureus. (1970) J Biol Chem, pp. 3697-3702 
504 |a Leloir, Staneloni, Carminatti, Behrens, (1973) Biochem. Biophys. Res. Commun, 52, pp. 1285-1292 
504 |a Lucas, Watacher, Lennarz, (1975) J. Biol. Chem, 250, pp. 1992-2002 
504 |a Ghalambor, Warren, Jeanloz, (1974) Biochem. Biophys. Res. Commun, 56, pp. 407-414 
504 |a García, Recondo, Dankert, Polysaccharide Biosynthesis in Acetobacter xylinum. Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars (1974) European Journal of Biochemistry, 43, pp. 93-105 
520 3 |a Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were equal to those of dolichol monophosphate. The glycosylated compounds were acid labile. Treatment with alkali of the acetylglucosaminyl compound produced a substance that migrated like a hexose phosphate on electrophoresis and that liberated acetylglucosamine on treatment with alkaline phosphatase. The behaviour of the insect glucosylated lipid on thin-layer chromatography and its stability to phenol were similar to dolichol monophosphate glucose and different from ficaprenyl monophosphate glucose. It is concluded that the insect glycosyl acceptor lipid is an α saturated polyprenyl phosphate. © 1975.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Acknowledgements. This investigation was supported by grants from the Consejo Wacional de Inveatigaaionee Cientfficas y Tdcnicaa (Argentina) and the Banco de la Ciudad de Buenos Aires. We thank Dr. Luis F. Leloir and the other members of the Institute de Investigacionee Bioqufmicas for their advice and support. 
593 |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina 
690 1 0 |a LIPID 
690 1 0 |a URIDINE DIPHOSPHATE GLUCOSE 
690 1 0 |a URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE 
690 1 0 |a ARTHROPOD 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a LIVER 
690 1 0 |a MICROSOME 
690 1 0 |a RAT 
690 1 0 |a THEORETICAL STUDY 
690 1 0 |a ACETYLGLUCOSAMINE 
690 1 0 |a ANIMAL 
690 1 0 |a CHROMATOGRAPHY, THIN LAYER 
690 1 0 |a DIPTERA 
690 1 0 |a GLUCOSE 
690 1 0 |a GLYCOLIPIDS 
690 1 0 |a INSECTS 
690 1 0 |a PHOSPHOLIPIDS 
690 1 0 |a RATS 
690 1 0 |a SPECIES SPECIFICITY 
700 1 |a Belocopitow, E. 
700 1 |a Maréchal, L.R. 
773 0 |d 1975  |g v. 66  |h pp. 1201-1208  |k n. 4  |p Biochem. Biophys. Res. Commun.  |x 0006291X  |w (AR-BaUEN)CENRE-905  |t Biochemical and Biophysical Research Communications 
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