Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli

Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Algranati, I.D
Otros Autores: Goldemberg, S.H
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1977
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
LEADER 05865caa a22008297a 4500
001 PAPER-12318
003 AR-BaUEN
005 20230518204231.0
008 190411s1977 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0017388330 
024 7 |2 cas  |a Magnesium, 7439-95-4; Polyamines; Putrescine, 110-60-1; RNA, Messenger 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a BBRCA 
100 1 |a Algranati, I.D. 
245 1 0 |a Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli 
260 |c 1977 
270 1 0 |m Algranati, I.D.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Tabor, Tabor, (1976) Ann. Rev. Biochem, 45, pp. 285-306 
504 |a Cohen, (1971) Introduction to the Polyamines, , Prentice-Hall, Inc, Englewood Cliffs, N.J 
504 |a Bachrach, (1973) Function of Naturally Occurring Polyamines, , Academic Press, New York 
504 |a Russell, (1973) Polyamines in Normal and Neoplastic Growth, pp. 1-13. , D.H. Russell, Raven Press, New York 
504 |a Takeda, Igarashi, (1969) Biochem. Biophys. Res. Commun, 37, pp. 917-924 
504 |a Algranati, Lengyel, (1966) J. Biol. Chem, 241, pp. 1778-1783 
504 |a Igarashi, Sugawara, Izumi, Nagayama, Hirose, (1974) Eur. J. Biochem, 48, pp. 495-502 
504 |a Takeda, (1969) Biochim. Biophys. Acta, 179, pp. 232-234 
504 |a Konecki, Kramer, Pinphanichakarn, Hardesty, (1975) Arch. Biochem. Biophys, 169, pp. 192-198 
504 |a Atkins, Lewis, Anderson, Gesteland, (1975) J. Biol. Chem, 250, pp. 5688-5695 
504 |a Hirshfield, Rosenfeld, Leifer, Maas, (1970) J. Bacteriol, 101, pp. 725-730 
504 |a Morris, Jorstad, (1970) J. Bacteriol, 101, pp. 731-737 
504 |a Young, Srinivasan, (1972) J. Bacteriol, 112, pp. 30-39 
504 |a Morris, Hansen, (1973) J. Bacteriol, 116, pp. 588-592 
504 |a Young, Srinivasan, (1974) J. Bacteriol, 117, pp. 1280-1288 
504 |a Echandi, Algranati, (1975) Biochem. Biophys. Res. Commun, 62, pp. 313-319 
504 |a Echandi, Algranati, (1975) Biochem. Biophys. Res. Commun, 67, pp. 1185-1191 
504 |a Algranati, Echandi, Goldemberg, Cunningham-Rundles, Maas, (1975) J. Bacteriol, 124, pp. 1122-1127 
504 |a Studier, (1973) J. Mol. Biol, 79, pp. 237-248 
504 |a Viñuela, Algranati, Ochoa, (1967) Eur. J. Biochem, 1, pp. 3-11 
504 |a Nathans, Oeschger, Eggen, Shimura, BACTERIOPHAGE-SPECIFIC PROTEINS IN E. COLI INFECTED WITH AN RNA BACTERIOPHAGE (1966) Proceedings of the National Academy of Sciences, 56, pp. 1844-1851 
504 |a Viñuela, Salas, Ochoa, TRANSLATION OF THE GENETIC MESSAGE, III. FORMYLMETHIONINE AS INITIATOR OF PROTEINS PROGRAMED BY POLYCISTRONIC MESSENGER RNA (1967) Proceedings of the National Academy of Sciences, 57, pp. 729-734 
520 3 |a Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity for the binding of formyl-methionyl-tRNA to ribosomes, induced by the trinucleotide AUG. The analysis of polypeptides formed showed that extracts from polyamine-depleted and supplemented cells are both able to synthesize complete protein chains. © 1977.  |l eng 
536 |a Detalles de la financiación: John Simon Guggenheim Memorial Foundation 
536 |a Detalles de la financiación: de are grateful to i)rs. L. F. Leloir, Y. Garcia-Patrone, A. J. Parodi, R, Piras and all other memberso f the Instituto de Investigacionea Bioqufmi-aas for their helpful discussions and advice. This investigation was partially aupported by grants from Pundacibn Runge % Born and the Consejo National de Investigaciones Cientfficaa v TBcnicas (Argentina). The authors are Career Investigators of the latter institution. I. D. A. acknowledges a fellowship from the John Simon Cuqgenheim Memorial Poundation during part of this work. 
593 |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina 
690 1 0 |a MESSENGER RNA 
690 1 0 |a POLYAMINE 
690 1 0 |a BACTERIOPHAGE 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a FORMYLMETHIONYL TRANSFER RIBONUCLEIC ACID 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a MICROORGANISM 
690 1 0 |a RNA TRANSLATION 
690 1 0 |a THEORETICAL STUDY 
690 1 0 |a COLIPHAGES 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a KINETICS 
690 1 0 |a MAGNESIUM 
690 1 0 |a POLYAMINES 
690 1 0 |a PUTRESCINE 
690 1 0 |a RIBOSOMES 
690 1 0 |a RNA, MESSENGER 
690 1 0 |a TRANSLATION, GENETIC 
700 1 |a Goldemberg, S.H. 
773 0 |d 1977  |g v. 75  |h pp. 1045-1051  |k n. 4  |p Biochem. Biophys. Res. Commun.  |x 0006291X  |w (AR-BaUEN)CENRE-905  |t Biochemical and Biophysical Research Communications 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0017388330&doi=10.1016%2f0006-291X%2877%2991487-5&partnerID=40&md5=a3856e138a9049c2a35e086dfa66a186  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1016/0006-291X(77)91487-5  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v75_n4_p1045_Algranati  |y Registro en la Biblioteca Digital 
961 |a paper_0006291X_v75_n4_p1045_Algranati  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 73271