Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemente...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1981
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04961caa a22007937a 4500 | ||
|---|---|---|---|
| 001 | PAPER-12200 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204224.0 | ||
| 008 | 190411s1981 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0019784427 | |
| 024 | 7 | |2 cas |a putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; spermine, 306-67-2, 71-44-3; Putrescine, 110-60-1; RNA, Transfer, Amino Acyl; tRNA, formylmethionine- | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBRCA | ||
| 100 | 1 | |a Algranati, I.D. | |
| 245 | 1 | 0 | |a Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| 260 | |c 1981 | ||
| 270 | 1 | 0 | |m Algranati, I.D.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Lovgren, Pettersson, Loftfield, (1978) J. Biol. Chem, 253, pp. 6702-6710 | ||
| 504 | |a Takeda, Igarashi, (1969) Biochem. Biophys. Res. Commun, 37, pp. 917-924 | ||
| 504 | |a Algranati, Goldemberg, (1977) Biochem. Biophys. Res. Commun, 75, pp. 1045-1051 | ||
| 504 | |a Igarashi, Yabuki, Yoshioka, Eguchi, Hirose, (1977) Biochem. Biophys. Res. Commun, 75, pp. 163-171 | ||
| 504 | |a Atkins, Lewis, Anderson, Gesteland, (1975) J. Biol. Chem, 250, pp. 5688-5695 | ||
| 504 | |a Konecki, Kramer, Pinphanichakarn, Hardesty, (1975) Arch. Biochem. Biophys, 169, pp. 192-198 | ||
| 504 | |a Hunter, Farrell, Jackson, Hunt, (1977) Eur. J. Biochem, 75, pp. 149-157 | ||
| 504 | |a Jelenc, Kurland, (1979) Proc. Natl. Acad. Sci. U.S.A, 76, pp. 3174-3178 | ||
| 504 | |a Goldemberg, Algranati, Polyamine requirement for streptomycin action on protein synthesis in bacteria. (1981) Eur J Biochem, , (in press) | ||
| 504 | |a Quigley, Teeter, Rich, (1978) Proc. Natl. Acad. Sci. U.S.A, 75, pp. 64-68 | ||
| 504 | |a Echandi, Algranati, (1975) Biochem. Biophys. Res. Commun, 62, pp. 313-319 | ||
| 504 | |a Echandi, Algranati, (1975) Biochem. Biophys. Res. Commun, 67, pp. 1185-1191 | ||
| 504 | |a Algranati, Goldemberg, (1977) Trends in Biochemical Sciences, 2, pp. 272-274 | ||
| 504 | |a Goldemberg, Algranati, (1977) Mol. Cell Biochem, 16, pp. 71-77 | ||
| 504 | |a Pestka, (1974) Methods Enzymol, 30, pp. 479-488 | ||
| 504 | |a Hobden, Cundliffe, (1978) Biochem. J, 170, pp. 57-61 | ||
| 504 | |a Darnbrough, Legon, Hunt, Jackson, (1973) J. Mol. Biol, 76, pp. 379-403 | ||
| 504 | |a Abraham, Olsnes, Pihl, (1979) FEBS Lett, 101, pp. 93-96 | ||
| 520 | 3 | |a Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemented bacteria. The markedly reduced activity in systems from polyamine starved cells is most probably due to a poorly efficient initiation step. The estimation of nascent peptide chains and their release from tRNA support the idea that polypeptide elongation and termination processes do not have decreased rates in systems from polyamine-depleted bacteria. © 1981. |l eng | |
| 593 | |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a PUTRESCINE |
| 690 | 1 | 0 | |a SPERMIDINE |
| 690 | 1 | 0 | |a SPERMINE |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a AUXOTROPHIC MUTANT |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a HEREDITY |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS |
| 690 | 1 | 0 | |a CELL-FREE SYSTEM |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MUTATION |
| 690 | 1 | 0 | |a PEPTIDE CHAIN ELONGATION |
| 690 | 1 | 0 | |a PEPTIDE CHAIN INITIATION |
| 690 | 1 | 0 | |a PEPTIDE CHAIN TERMINATION |
| 690 | 1 | 0 | |a PUTRESCINE |
| 690 | 1 | 0 | |a RIBOSOMES |
| 690 | 1 | 0 | |a RNA, TRANSFER, AMINO ACYL |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 700 | 1 | |a Goldemberg, S.H. | |
| 773 | 0 | |d 1981 |g v. 103 |h pp. 8-15 |k n. 1 |p Biochem. Biophys. Res. Commun. |x 0006291X |w (AR-BaUEN)CENRE-905 |t Biochemical and Biophysical Research Communications | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019784427&doi=10.1016%2f0006-291X%2881%2991653-3&partnerID=40&md5=38e43d4bc5dd568035ae5480dfade9f5 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0006-291X(81)91653-3 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v103_n1_p8_Algranati |y Registro en la Biblioteca Digital |
| 961 | |a paper_0006291X_v103_n1_p8_Algranati |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 73153 | ||