Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with...
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| Formato: | Capítulo de libro |
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1983
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 08097caa a22011537a 4500 | ||
|---|---|---|---|
| 001 | PAPER-12166 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204222.0 | ||
| 008 | 170724s1983 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0021072880 | |
| 024 | 7 | |2 cas |a hydroxymethylglutaryl coenzyme A reductase, 37250-24-1; Acetates; Acetyl Coenzyme A, 72-89-9; Cholesterol, 57-88-5; Hydroxymethylglutaryl CoA Reductases, EC 1.1.1.- | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MCEND | ||
| 100 | 1 | |a Pignataro, O.P. | |
| 245 | 1 | 0 | |a Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
| 260 | |c 1983 | ||
| 270 | 1 | 0 | |m Pignataro, O.P. |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Ascoli, (1981) J. Lipid Res., 22, pp. 1247-1253 | ||
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| 504 | |a van der Molen, Rommerts, (1981) The Testis, pp. 213-238. , H. Burger, D. de Kretser, Raven Press, New York | ||
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| 504 | |a van der Vusse, Kalkman, van der Molen, (1975) Biochim. Biophys. Acta, 380, pp. 473-485 | ||
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| 520 | 3 | |a The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983. |l eng | |
| 593 | |a Instituto de Biologia y Medicina Experimental and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos AiresArgentina | ||
| 690 | 1 | 0 | |a CHOLESTEROL BIOSYNTHESIS |
| 690 | 1 | 0 | |a HMG-COA REDUCTASE |
| 690 | 1 | 0 | |a LEYDIG CELL |
| 690 | 1 | 0 | |a HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a ACETYL COENZYME A C 14 |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a BIOLOGICAL MODEL |
| 690 | 1 | 0 | |a CHOLESTEROL SYNTHESIS |
| 690 | 1 | 0 | |a ENDOCRINE SYSTEM |
| 690 | 1 | 0 | |a LEYDIG CELL |
| 690 | 1 | 0 | |a MALE GENITAL SYSTEM |
| 690 | 1 | 0 | |a MITOCHONDRION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ACETATES |
| 690 | 1 | 0 | |a ACETYL COENZYME A |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CELL COMPARTMENTATION |
| 690 | 1 | 0 | |a CHOLESTEROL |
| 690 | 1 | 0 | |a HYDROXYMETHYLGLUTARYL COA REDUCTASES |
| 690 | 1 | 0 | |a LEYDIG CELLS |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MITOCHONDRIA |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 700 | 1 | |a Radicella, J.P. | |
| 700 | 1 | |a Calvo, J.C. | |
| 700 | 1 | |a Charreau, E.H. | |
| 773 | 0 | |d 1983 |g v. 33 |h pp. 53-67 |k n. 1 |x 03037207 |w (AR-BaUEN)CENRE-6138 |t Mol. Cell. Endocrinol. | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro |y Registro en la Biblioteca Digital |
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