Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase
A system was developed for testing the effect of phospholipids on dolichyl phosphate phosphatase, a membrane-associated enzyme. This enzyme was solubilized, delipidated, stabilized and concentrated in such a way that minimal quantities of Triton X-100 were carried by enzyme extracts to the incubatio...
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| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1984
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 06352caa a22009617a 4500 | ||
|---|---|---|---|
| 001 | PAPER-12136 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204220.0 | ||
| 008 | 170724s1984 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0021278504 | |
| 024 | 7 | |2 cas |a Catalase, EC 1.11.1.6; Cytochrome c, 9007-43-6; Detergents; dolichyl-phosphatase, EC 3.1.3.51; Octoxynol, 9002-93-1; Phospholipids; Phosphoric Monoester Hydrolases, EC 3.1.3; Polyethylene Glycols; Surface-Active Agents | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBLLA | ||
| 100 | 1 | |a Boscoboinik, D.O. | |
| 245 | 1 | 0 | |a Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase |
| 260 | |c 1984 | ||
| 270 | 1 | 0 | |m Belocopitow, E. |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Parodi, Leloir, (1979) Biochim. Biophys. Acta, 559, pp. 1-37 | ||
| 504 | |a Lucas, Levin, (1977) J. Biol. Chem., 252, pp. 4330-4336 | ||
| 504 | |a Krag, Cifone, Robbins, Rayman, (1977) J. Biol. Chem., 252, pp. 3561-3564 | ||
| 504 | |a Carson, Lennarz, (1981) J. Biol. Chem., 256, pp. 4679-4686 | ||
| 504 | |a Hartford, Waechter, Earl, (1977) Biochem. Biophys. Res. Commun., 76, pp. 1036-1043 | ||
| 504 | |a Wedgwood, Strominger, (1980) J. Biol. Chem., 255, pp. 1120-1123 | ||
| 504 | |a Idoyaga Vargas, Belocopitow, Mentaberry, Carminatti, (1980) FEBS Lett., 112, pp. 63-65 | ||
| 504 | |a Rip, Rupar, Chaudhary, Carrol, (1981) J. Biol. Chem., 256, pp. 1929-1934 | ||
| 504 | |a Burton, Scher, Waechter, (1981) Arch. Biochem. Biophys., 208, pp. 409-417 | ||
| 504 | |a Belocopitow, Boscoboinik, (1982) Eur. J. Biochem., 125, pp. 167-173 | ||
| 504 | |a Racker, Violand, O'Neal, Alfonzo, Telford, (1979) Arch. Biochem. Biophys., 198, pp. 470-477 | ||
| 504 | |a Folch, Less, Sloane, (1957) J. Biol. Chem., 226, pp. 497-509 | ||
| 504 | |a Boscoboinik, Belocopitow, (1981) Anal. Biochem., 114, pp. 42-45 | ||
| 504 | |a Hess, Derr, (1975) Anal. Biochem., 63, pp. 607-613 | ||
| 504 | |a Terenzi, Roselino, Passeron, (1971) Eur. J. Biochem., 18, pp. 342-350 | ||
| 504 | |a Worthington Biochemical Corporation, (1972) Worthington Enzyme Manual, pp. 7-12. , 5th Edn., Worthington, Freehold, NJ | ||
| 504 | |a Clarke, (1975) J. Biol. Chem., 250, pp. 5459-5463 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Batzri, Korn, (1973) Biochim. Biophys. Acta, 298, pp. 1015-1019 | ||
| 504 | |a Letoublon, Frot Coutaz, Got, (1981) Carbohydr. Res., 89, pp. 145-150 | ||
| 504 | |a Hasselbeck, Tanner, Dolichyl phosphate-mediated mannosyl transfer through liposomal membranes. (1982) Proceedings of the National Academy of Sciences, 79, pp. 1520-1524. , 5th Edn | ||
| 504 | |a Sanderman, Jr., Regulation of membrane enzymes by lipids (1978) Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 515, pp. 209-237 | ||
| 504 | |a Helenius, Simons, (1975) Biochim. Biophys. Acta, 415, pp. 29-79 | ||
| 504 | |a Jensen, Schultzbach, (1982) J. Biol. Chem., 257, pp. 9025-9029 | ||
| 504 | |a Possmayer, Scherhof, Dubbelman, Van Golde, Van Deenen, (1969) Biochim. Biophys. Acta, 176, pp. 95-110 | ||
| 504 | |a Ravi, Rip, Carroll, (1983) Biochem. J., 213, pp. 513-518 | ||
| 520 | 3 | |a A system was developed for testing the effect of phospholipids on dolichyl phosphate phosphatase, a membrane-associated enzyme. This enzyme was solubilized, delipidated, stabilized and concentrated in such a way that minimal quantities of Triton X-100 were carried by enzyme extracts to the incubation mixture. Its substrate, dolichyl phosphate, could be kept in aqueous medium as suspended particles without addition of detergent. When dolichyl phosphate phosphatase was assayed using the substrate in this detergent-free form, values for Km, pH optimum and temperature optimum were different from those obtained with detergentsolubilized substrate. This assay of dolichyl phosphate phosphatase almost free of detergent allowed testing of the effect of specific phospholipids on enzyme activity with minimal interference produced by endogenous phospholipids or exogenous detergent. Sphingomyelin, phosphatidylethanolamine or phosphatidylcholine (zwitterionic phospholipids) acted as activators, whereas phosphatidic acid and phosphatidylinositol, negatively-charged phospholipids, were inhibitors of dolichyl phosphate phosphatase. © 1984. |l eng | |
| 593 | |a Instituto de Investigaciones Bioquimicas 'Fundacion Campomar' and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos AiresArgentina | ||
| 690 | 1 | 0 | |a (RAT LIVER MICROSOME) |
| 690 | 1 | 0 | |a DETERGENT |
| 690 | 1 | 0 | |a DOLICHYL PHOSPHATE PHOSPHATASE |
| 690 | 1 | 0 | |a ENZYME ASSAY |
| 690 | 1 | 0 | |a PHOSPHOLIPID |
| 690 | 1 | 0 | |a PHOSPHOLIPID |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a DOLICHYL PHOSPHATASE |
| 690 | 1 | 0 | |a ENZYME ASSAY |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a MICROSOME |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CATALASE |
| 690 | 1 | 0 | |a CYTOCHROME C |
| 690 | 1 | 0 | |a DETERGENTS |
| 690 | 1 | 0 | |a INTRACELLULAR MEMBRANES |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MICROSOMES, LIVER |
| 690 | 1 | 0 | |a OCTOXYNOL |
| 690 | 1 | 0 | |a PHOSPHOLIPIDS |
| 690 | 1 | 0 | |a PHOSPHORIC MONOESTER HYDROLASES |
| 690 | 1 | 0 | |a POLYETHYLENE GLYCOLS |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a SURFACE-ACTIVE AGENTS |
| 700 | 1 | |a Morera, S. | |
| 700 | 1 | |a Belocopitow, E. | |
| 773 | 0 | |d 1984 |g v. 794 |h pp. 41-48 |k n. 1 |x 00052760 |w (AR-BaUEN)CENRE-2261 |t Biochim. Biophys. Acta Lipids Lipid Metab. | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021278504&doi=10.1016%2f0005-2760%2884%2990295-9&partnerID=40&md5=50adea564ec6d50e502ca96f9bcea31f |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0005-2760(84)90295-9 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00052760_v794_n1_p41_Boscoboinik |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v794_n1_p41_Boscoboinik |y Registro en la Biblioteca Digital |
| 961 | |a paper_00052760_v794_n1_p41_Boscoboinik |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 73089 | ||