cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii

Intracellular levels of cAMP and specific activities of adenylate cyclase and cAMP phosphodiesterase were measured during yeast-to-hyphae transition in the dimorphic fungus M. rouxii. Enzymatic activities were measured in permeabilized cells under conditions preventing protein dephosphorylation and...

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Autor principal: Cantore, M.L
Otros Autores: Galvagno, M.A, Passeron, S.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1983
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0020857042 
024 7 |2 cas  |a 3',5' cyclic nucleotide phosphodiesterase, 9040-59-9; adenylate cyclase, 9012-42-4; bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Adenylate Cyclase, EC 4.6.1.1; Bucladesine, 362-74-3; Cyclic AMP, 60-92-4 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a CBRPD 
100 1 |a Cantore, M.L. 
245 1 0 |a cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii 
260 |c 1983 
270 1 0 |m Passeron, S. 
506 |2 openaire  |e Política editorial 
504 |a Cantore, Galvagno, Passeron, Variations in the levels of cyclic adenosine 3′–5′ monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3′–5′ monophosphate phosphodiesterase during aerobic morphogenesis of Mueor rouxii (1980) Archives of Biochemistry and Biophysics, 199, pp. 312-320 
504 |a Cantore, Passeron, Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase (1982) Archives of Biochemistry and Biophysics, 219, pp. 1-11 
504 |a Galvagno, Moreno, Cantore, Passeron, Cyclic adenosine 3′–5′ monophosphate phosphodiesterase from Mucor rouxii: regulation of enzyme activity by phosphorylation and dephosphorylation (1979) Biochemical and Biophysical Research Communications, 89, pp. 779-785 
504 |a Larsen, Sypherd, Cyclic adenosine 3′,5′-monophosphate and morphogenesis in Mucor racemosus (1974) Journal of Bacteriology, 117, pp. 432-438 
504 |a Lowry, Rosebrough, Farr, Randall, Protein measurement with Folin phenol reagent (1951) Journal of Biological Chemistry, 193, pp. 265-275 
504 |a Maggese, Galvagno, Cantore, Passeron, In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii (1982) Cell Biology International Reports, 6, pp. 1101-1108 
504 |a Moreno, Galvagno, Passeron, Control of Mucor rouxii adenosine 3′–5′ monophosphate phosphodiesterase by phosphorylation-dephosphorylation and proteolysis (1982) Archives of Biochemistry and Biophysics, 214, pp. 573-580 
504 |a Paveto, Epstein, Passeron, Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii (1975) Archives of Biochemistry and Biophysics, 169, pp. 449-456 
504 |a Sypherd, Borgia, Paznokas, Biochemistry of dimorphism in the fungus Mucor (1978) Advances in Microbiological Physiology, 18, pp. 67-104 
504 |a Tsang, Lehotay, Pearson Murphy, Competitive binding assay for adenosine 3′,5′-monophosphate employing a bovine adrenal protein: application to urine, plasma and tissues (1972) The Journal of Clinical Endocrinology and Metabolism, 35, pp. 809-817 
520 3 |a Intracellular levels of cAMP and specific activities of adenylate cyclase and cAMP phosphodiesterase were measured during yeast-to-hyphae transition in the dimorphic fungus M. rouxii. Enzymatic activities were measured in permeabilized cells under conditions preventing protein dephosphorylation and proteolysis. A two-fold decrease in intracellular cAMP levels occurred shortly after exposure of the yeast to air quite before morphological changes became evident. Morphogenesis to hyphae after exposure to air was inhibited by the addition of 10 mM dibutyryl cAMP to the culture medium, and the yeast morphology was maintained for at least 24 hours. The decrease in cAMP levels that occurs shortly after exposure of yeast culture to air was mainly accounted for by variations in the state of activation of cAMP phosphodiesterase while the specific activity of adenylate cyclase did not vary significantly during yeast-to-hyphae transition. © 1983.  |l eng 
593 |a Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica y Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina 
593 |a Career Investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina 
690 1 0 |a 3',5' CYCLIC NUCLEOTIDE PHOSPHODIESTERASE 
690 1 0 |a ADENYLATE CYCLASE 
690 1 0 |a BUCLADESINE 
690 1 0 |a CYCLIC AMP 
690 1 0 |a ARTICLE 
690 1 0 |a COMPARATIVE STUDY 
690 1 0 |a DRUG EFFECT 
690 1 0 |a KINETICS 
690 1 0 |a METABOLISM 
690 1 0 |a MUCOR 
690 1 0 |a PHYSIOLOGY 
690 1 0 |a SPECIES DIFFERENCE 
690 1 0 |a 3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 
690 1 0 |a ADENYLATE CYCLASE 
690 1 0 |a BUCLADESINE 
690 1 0 |a COMPARATIVE STUDY 
690 1 0 |a CYCLIC AMP 
690 1 0 |a KINETICS 
690 1 0 |a MUCOR 
690 1 0 |a SPECIES SPECIFICITY 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
700 1 |a Galvagno, M.A. 
700 1 |a Passeron, S. 
773 0 |d 1983  |g v. 7  |h pp. 947-954  |k n. 11  |p Cell Biol. Int. Rep.  |x 03091651  |t Cell Biology International Reports 
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856 4 0 |u https://doi.org/10.1016/0309-1651(83)90214-X  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03091651_v7_n11_p947_Cantore  |y Handle 
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