Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants

The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the...

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Detalles Bibliográficos
Autor principal: Resnicoff, M.
Otros Autores: Medrano, E.E
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1989
Acceso en línea:Registro en Scopus
DOI
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
LEADER 06331caa a22009617a 4500
001 PAPER-11997
003 AR-BaUEN
005 20230518204212.0
008 190411s1989 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0024516933 
024 7 |2 cas  |a butyric acid, 107-92-6, 156-54-7, 461-55-2; estradiol, 50-28-2; insulin, 9004-10-8; prostaglandin F2 alpha, 551-11-1; thrombin, 9002-04-4; 13-cis-retinal, 472-86-6; Butyric Acid, 107-92-6; Butyric Acids; Culture Media; Dinoprost, 551-11-1; DNA, 9007-49-2; Estradiol, 50-28-2; Hormones; Insulin, 11061-68-0; Retinaldehyde, 116-31-4; Retinoids; Thrombin, EC 3.4.21.5 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
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100 1 |a Resnicoff, M. 
245 1 0 |a Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants 
260 |c 1989 
270 1 0 |m Medrano, E.E.; Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales (UBA), Patricias Argentinas 435, 1405 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Hepner, (1984) Cancer Res, 44, pp. 2259-2265 
504 |a Buick, Pollack, (1984) Cancer Res, 44, pp. 4909-4918 
504 |a Resnicoff, Medrano, Podhajcer, Bravo, Bover, Mordoh, (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 7295-7299 
504 |a Medrano, Cafferata, Larcher, (1987) Exp. Cell Res, 172, pp. 354-364 
504 |a Medrano, E. E., Resnicoff, M., Larcher, F., Cafferata, E. G. A., Podhajcer, O. L., Bover, L., and Molinari, submitted for publication; Resnicoff, Medrano, (1987) Biochem. Biophys. Res. Commun, 143, pp. 309-315 
504 |a Podhajcer, Resnicoff, Bover, Medrano, Slavutsky, Larripa, Mordoh, (1988) Exp Cell Res, , in press 
504 |a Gomez, Medrano, Cafferata, Tellez-Inon, (1988) Exp. Cell Res, 175, pp. 74-80 
504 |a Raben, Yasuda, Cunningham, (1987) Journal of Cellular Physiology, 130, pp. 468-473 
504 |a L'Allemain, Paris, Magnaldo, Poyssegur, (1986) J. Cell. Physiol, 129, pp. 167-174 
504 |a Carney, Scott, Gordon, La Belle, (1985) Cell, 42, pp. 479-488 
504 |a Davis, Weakland, Weiland, Farese, West, (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 3728-3732 
504 |a Macphee, Drummond, Otto, Jimenez de Asua, (1984) J. Cell. Physiol, 119, pp. 35-40 
504 |a Dickson, Kasid, Huff, Bates, Knabbe, Bronzert, Gelmann, Lippman, (1987) Proc. Natl. Acad. Sci USA, 84, pp. 837-841 
504 |a Knabbe, Lippman, Wakefield, Flanders, Kasid, Derynck, Dickson, (1987) Cell, 48, pp. 417-428 
504 |a Abe, Kufe, (1986) J. Cell. Physiol, 126, pp. 126-132 
520 3 |a The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the response of the isolated subpopulations to insulin, thrombin, PGF2a, estradiol, and 13-cis-retinal. We demonstrate that the first two growth factors stimulate [3H]thymidine incorporation in the more differentiated subpopulations (D and F), while PGF2a has mitogenic activity in subpopulations C and D. In the absence of any added growth factor, estradiol has the extreme and transient capacity of allowing the stem cell to detach from the tissue culture dish and to grow in suspension as multicellular aggregates (MCF-7/SE cells). 13-cis-Retinal acts as a negative modulator of differentiation and protects the cells from the inhibitory and differentiation activity of Na-butyrate. © 1989.  |l eng 
536 |a Detalles de la financiación: Support for this work was from the Consejo National de Investigaciones Cientiftcas y Tecnicas de Argentina (CONICET), and from the Fundacion Maria Calderon de la Barca. E.E.M. is a Career Researcher of the CONICET and M.R. is a Fellow of the Fundacion Maria Calderon de la Barca. We thank E. G. A. Cafferata for excellent technical assistance. We are grateful to Dr Luis Jimenez de Asua for critically reading the manuscript and to Maria Dolores Turro for typing it. 
593 |a Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales (UBA), Patricias Argentinas 435, 1405 Buenos Aires, Argentina 
690 1 0 |a 13 CIS RETINAL 
690 1 0 |a BUTYRIC ACID 
690 1 0 |a ESTRADIOL 
690 1 0 |a INSULIN 
690 1 0 |a PROSTAGLANDIN F2 ALPHA 
690 1 0 |a RADIOISOTOPE 
690 1 0 |a THROMBIN 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a BREAST CANCER 
690 1 0 |a CELL CULTURE 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a CELL GROWTH 
690 1 0 |a CELL STRAIN MCF 7 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CYTOLOGY 
690 1 0 |a FEMALE 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN CELL 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a BREAST NEOPLASMS 
690 1 0 |a BUTYRIC ACID 
690 1 0 |a BUTYRIC ACIDS 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a CELL DIVISION 
690 1 0 |a CELL SURVIVAL 
690 1 0 |a CULTURE MEDIA 
690 1 0 |a DINOPROST 
690 1 0 |a DNA 
690 1 0 |a ESTRADIOL 
690 1 0 |a HORMONES 
690 1 0 |a HUMAN 
690 1 0 |a INSULIN 
690 1 0 |a RETINALDEHYDE 
690 1 0 |a RETINOIDS 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a THROMBIN 
690 1 0 |a TUMOR CELLS, CULTURED 
690 1 0 |a TUMOR STEM CELLS 
700 1 |a Medrano, E.E. 
773 0 |d 1989  |g v. 181  |h pp. 116-125  |k n. 1  |p Exp. Cell Res.  |x 00144827  |w (AR-BaUEN)CENRE-797  |t Experimental Cell Research 
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856 4 0 |u https://hdl.handle.net/20.500.12110/paper_00144827_v181_n1_p116_Resnicoff  |y Handle 
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