Changes induced by glucose in the plasma membrane properties of pancreatic islets

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Partially purified membranes obtained from rat pancreatic isolated islets preincubated for 3 min with 3.3 and 16.6 mM glucose were labelled with l,6-diphenyl-l,3,5-hexatriene to study fluorescence polarization. Other islets, incubated for 5 min with the same glucose concentration, were extracted and...

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Detalles Bibliográficos
Autor principal: Cortizo, A.M
Otros Autores: Paladini, A., Diaz, G.B, Garcia, M.E, Gagliardino, J.J
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1990
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 scopus  |a 2-s2.0-0025358854 
024 7 |2 cas  |a Diphenylhexatriene, 1720-32-7; Fatty Acids; Glucose, 50-99-7; Phospholipids 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a MCEND 
100 1 |a Cortizo, A.M. 
245 1 0 |a Changes induced by glucose in the plasma membrane properties of pancreatic islets 
260 |c 1990 
270 1 0 |m Cortizo, A.M.; CENEXA-Centro de Endocrinologia Experimental y Aplicada (UNLP-CONICET), Facultad de Ciencias Medicos, UNLP, 60 y 120, 1900 La Plata, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Arvidson, (1968) Eur. J. Biochem., 4, pp. 478-486 
504 |a Best, Malaisse, (1983) Diabetologia, 25, pp. 299-305 
504 |a Cortizo, Garcia, Pasquini, Gagliardino, (1984) IRCS Med. Sci., 12, pp. 1117-1118 
504 |a Cortizo, Garcia, Gagliardino, (1987) Med. Sci. Res., 15, pp. 289-290 
504 |a Deleers, Ruyschaert, Malaisse, (1981) Biochem. Biophys. Res. Commun., 98, pp. 255-260 
504 |a Duzgunes, Wilschut, Fraley, Papahadjopoulos, Studies on the mechanism of membrane fusion. Role of head-group composition in calcium- and magnesium-induced fusion of mixed phospholipid vesicles (1981) Biochimica et Biophysica Acta (BBA) - Biomembranes, 642, pp. 182-195 
504 |a Folch, Lee, Sloane-Stanley, (1957) J. Biol. Chem., 226, pp. 497-509 
504 |a Gagliardino, (1983) Diabetes 1982, pp. 19-27. , E.N. Mngola, Excerpta Medica, Amsterdam 
504 |a Gagliardino, Borelli, de Gagliardino, Garcia, (1986) Diabetes Res. Clin. Pract., 1, pp. 327-333 
504 |a Gronda, Rossi, Gagliardino, (1987) Acta Physiol. Pharmacol. Latinoam., 37, pp. 245-256 
504 |a Grunberger, Haimovitz, Shinitzky, (1982) Biochim. Biophys. Acta, 688, pp. 764-774 
504 |a Kimelberg, (1982) Physical Methods in Biological Membranes and their Model System, pp. 261-276. , NATO Advanced Study Institute Series/Plenum Press, New York 
504 |a Lacy, (1961) Am. J. Med., 31, pp. 851-859 
504 |a Lacy, Kostianovsky, (1967) Diabetes, 16, pp. 35-39 
504 |a Lakowicz, Prendergast, Högen, (1979) Biochemistry, 18, pp. 508-519 
504 |a Lakowicz, Prendergast, Hogen, (1979) Biochemistry, 18, pp. 520-527 
504 |a Livingstone, Schachter, (1980) J. Biol. Chem., 255, pp. 10902-10908 
504 |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem., 193, pp. 265-275 
504 |a Montague, Parkin, (1980) Horm. Metab. Res., 10, pp. 153-157. , (Suppl.) 
504 |a Orci, (1982) Diabetes, 31, pp. 538-565 
504 |a Roos, Chopin, (1985) J. Cell Biol., 101, pp. 1591-1598 
504 |a Shinitzky, Barenholz, (1974) J. Biol. Chem., 249, pp. 2652-2657 
504 |a Shinitzky, Barenholz, (1978) Biochim. Biophys. Acta, 515, pp. 367-394 
504 |a Shinitzky, Inbar, (1976) Biochim. Biophys. Acta, 433, pp. 133-149 
504 |a Stoffel, Chu, Ahrens, Jr., (1959) Anal. Chem., 31, pp. 307-308 
504 |a Sundler, Duzgunes, Papahadjopoulos, (1981) Biochim. Biophys. Acta, 649, pp. 751-758 
504 |a Van Dorp, Beerthuis, Nugteren, Vankernan, Jr., (1964) Biochim. Biophys. Acta, 90, pp. 204-206 
504 |a Weber, Photoelectric Method for the Measurement of the Polarization of the Fluorescence of Solutions (1956) Journal of the Optical Society of America, 46, pp. 962-970 
520 3 |a Partially purified membranes obtained from rat pancreatic isolated islets preincubated for 3 min with 3.3 and 16.6 mM glucose were labelled with l,6-diphenyl-l,3,5-hexatriene to study fluorescence polarization. Other islets, incubated for 5 min with the same glucose concentration, were extracted and phospholipids separated by thin-layer chromatography. The composition of phospholipids of fatty acids was then studied by gas-liquid chromatography. Arrhenius plots of the microviscosity in membranes obtained from islets exhibited two components, a steeper slope below 18 °C and a gentler slope above 18 °C, indicating greater flow activation energy at temperatures below the transition point. Exposure of islets to 16.6 mM glucose significantly increased the flow activation energy (ΔE), below and above the transition point. Islets incubated for 5 min with 16.6 mM glucose showed an increase in the percentage composition of 12 : 0 and 18 : 2 together with a decrease in the 20 : 2 W6 and 22 : 3 W3 fatty acids esterified to phospholipids. Regardless of these changes, no significant alterations occurred in the proportion of saturated fatty acids or in the double bond index; these measurements therefore did not account for the effects of glucose concentration in flow activation energy. The thermotropic changes reported here might be the consequence of some degree of disorder induced by glucose upon the membrane structure. This order alteration could either favor the membrane fusion which occurs during the emiocytosis or only reflects the consequence of such a process. © 1990.  |l eng 
593 |a CENEXA-Centro de Endocrinologia Experimental y Aplicada (UNLP-CONICET), Facultad de Ciencias Medicos, UNLP, 60 y 120, 1900 La Plata, Argentina 
593 |a INGEBI - Instituto de Investigaciones en Ingenieria Geneticay Biologia Molecular (UBA-FCEN-CONICET), Obligado, 2490 Buenos Aires, Argentina 
690 1 0 |a B CELL MEMBRANE 
690 1 0 |a FATTY ACIDS LIPID BILAYER ORDER 
690 1 0 |a FLUORESCENCE POLARIZATION 
690 1 0 |a PANCREATIC ISLET 
690 1 0 |a GLUCOSE 
690 1 0 |a MEMBRANE LIPID 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ARTICLE 
690 1 0 |a CELL MEMBRANE 
690 1 0 |a FLUORESCENCE POLARIZATION 
690 1 0 |a NONHUMAN 
690 1 0 |a PANCREAS ISLET BETA CELL 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a ANIMAL 
690 1 0 |a CELL MEMBRANE 
690 1 0 |a DIPHENYLHEXATRIENE 
690 1 0 |a FATTY ACIDS 
690 1 0 |a FLUORESCENCE POLARIZATION 
690 1 0 |a GLUCOSE 
690 1 0 |a ISLETS OF LANGERHANS 
690 1 0 |a MALE 
690 1 0 |a MEMBRANE FLUIDITY 
690 1 0 |a PHOSPHOLIPIDS 
690 1 0 |a RATS 
690 1 0 |a RATS, INBRED STRAINS 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TEMPERATURE 
690 1 0 |a THERMODYNAMICS 
690 1 0 |a VISCOSITY 
700 1 |a Paladini, A. 
700 1 |a Diaz, G.B. 
700 1 |a Garcia, M.E. 
700 1 |a Gagliardino, J.J. 
773 0 |d 1990  |g v. 71  |h pp. 49-54  |k n. 1  |p Mol. Cell. Endocrinol.  |x 03037207  |w (AR-BaUEN)CENRE-6138  |t Molecular and Cellular Endocrinology 
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856 4 0 |u https://doi.org/10.1016/0303-7207(90)90074-I  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03037207_v71_n1_p49_Cortizo  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v71_n1_p49_Cortizo  |y Registro en la Biblioteca Digital 
961 |a paper_03037207_v71_n1_p49_Cortizo  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 72885 

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