β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms h...
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| Otros Autores: | , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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2014
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 03611caa a22004457a 4500 | ||
|---|---|---|---|
| 001 | PAPER-10994 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204105.0 | ||
| 008 | 140217s2014 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84891893509 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MLBRB | ||
| 100 | 1 | |a Zubeldia-Brenner, L. | |
| 245 | 1 | 0 | |a β3-Chimaerin, a novel member of the chimaerin Rac-GAP family |
| 260 | |c 2014 | ||
| 270 | 1 | 0 | |m Leskow, F.C.; Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, 6700, Buenos Aires, Argentina; email: federico@fbmc.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain. © 2014 Springer Science+Business Media Dordrecht. |l eng | |
| 536 | |a Article in Press | ||
| 593 | |a Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, IQUIBICEN-CONICET, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina | ||
| 593 | |a Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, 19104-6160, United States | ||
| 593 | |a Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, 6700, Argentina | ||
| 690 | 1 | 0 | |a C1 DOMAIN |
| 690 | 1 | 0 | |a CHIMAERIN |
| 690 | 1 | 0 | |a CHN2 |
| 690 | 1 | 0 | |a PHORBOL ESTERS |
| 690 | 1 | 0 | |a RAC-GAP |
| 700 | 1 | |a Gutierrez-Uzquiza, A. | |
| 700 | 1 | |a Barrio-Real, L. | |
| 700 | 1 | |a Wang, H. | |
| 700 | 1 | |a Kazanietz, M.G. | |
| 700 | 1 | |a Leskow, F.C. | |
| 773 | 0 | |d 2014 |h pp. 1-10 |p Mol. Biol. Rep. |x 03014851 |w (AR-BaUEN)CENRE-3497 |t Molecular Biology Reports | |
| 856 | 4 | 1 | |u http://www.scopus.com/inward/record.url?eid=2-s2.0-84891893509&partnerID=40&md5=2b86b91aa1ffbc25c043e8694942ebea |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s11033-014-3055-3 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03014851_v_n_p1_ZubeldiaBrenner |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014851_v_n_p1_ZubeldiaBrenner |y Registro en la Biblioteca Digital |
| 961 | |a paper_03014851_v_n_p1_ZubeldiaBrenner |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 71947 | ||