Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance
In order to study the intracellular polyamine distribution in Escherichia coli, 13C-NMR spectra of [1,4-13C]putrescine were obtained after addition of the latter to intact bacteria. The 13C-enriched methylene signal underwent line broadening. When the cells were centrifuged after 90 min the cell-bou...
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1984
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| LEADER | 06805caa a22009257a 4500 | ||
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| 001 | PAPER-1091 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203027.0 | ||
| 008 | 190411s1984 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0021760758 | |
| 024 | 7 | |2 cas |a carbon, 7440-44-0; putrescine, 110-60-1, 333-93-7; transfer RNA, 9014-25-9; Carbon Isotopes; DNA, Bacterial; Putrescine, 110-60-1; RNA, Transfer, 9014-25-9 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BAMRD | ||
| 100 | 1 | |a Frydman, B. | |
| 245 | 1 | 0 | |a Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance |
| 260 | |c 1984 | ||
| 270 | 1 | 0 | |m Frydman, B.; Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín, 956 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Cohen, (1971) Introduction to the Polyamines, , Prentice-Hall, Englewood Cliffs, NJ | ||
| 504 | |a Bachrach, (1973) Function of Naturally Occurring Polyamines, , Academic Press, New York | ||
| 504 | |a Tabor, Tabor, (1976) Annu. Rev. Biochem., 45, pp. 285-306 | ||
| 504 | |a Algranati, Goldemberg, (1977) Trends Biochem. Sci., 2, pp. 272-274 | ||
| 504 | |a Gabbay, Glaser, Gaffney, (1970) Annals of the New York Academy of Sciences, 171, pp. 810-826 | ||
| 504 | |a Cohen, Lichtenstein, (1960) J. Biol. Chem., 235, pp. 2112-2116 | ||
| 504 | |a Quigley, Teeter, Rich, (1978) Proc. Natl. Acad. Sci. USA, 75, pp. 64-68 | ||
| 504 | |a Tabor, Kellog, (1967) J. Biol. Chem., 242, pp. 1044-1052 | ||
| 504 | |a Stevens, McCann, (1970) Annals of the New York Academy of Sciences, 171, pp. 827-837 | ||
| 504 | |a Dion, Herbst, (1967) Proc. Natl. Acad. Sci. USA, 58, pp. 2367-2371 | ||
| 504 | |a Schulman, (1969) Biological Applications of Magnetic Resonance, , Academic Press, New York | ||
| 504 | |a Dwek, (1975) Nuclear Magnetic Resonance in Biochemistry, , Oxford University Press, London | ||
| 504 | |a Echandi, Algranati, (1975) Biochem. Biophys. Res. Commun., 62, pp. 313-319 | ||
| 504 | |a Murray, III, Williams, (1958) Organic Synthesis with Isotopes, p. 129. , Interscience, New York, Part I | ||
| 504 | |a Murray, III, Williams, (1958) Organic Synthesis with Isotopes, p. 507. , Interscience, New York, Part I | ||
| 504 | |a Levitt, Freeman, Frenkiel, (1982) J. Magn. Resonance, 47, pp. 328-330 | ||
| 504 | |a Canet, Levy, Peat, (1975) J. Magn. Resonance, 18, pp. 199-204 | ||
| 504 | |a Olsen, Schweitzer, Walkin, Hamil, Jr., Horton, Grant, (1982) Nucleic Acids Research, 10, pp. 4449-4464 | ||
| 504 | |a Martin, Martin, Delpuesch, (1980) Practical NMR Spectroscopy, pp. 15-18. , Heyden, London | ||
| 504 | |a Martin, Martin, Delpuesch, (1980) Practical NMR Spectroscopy, pp. 22-23. , Heyden, London | ||
| 504 | |a Rubin, (1977) J. Bacteriol., 129, pp. 916-925 | ||
| 504 | |a Gasule, Schellman, (1978) J. Mol. Biol., 121, pp. 311-326 | ||
| 504 | |a Pochon, Cohen, (1972) Biochem. Biophys. Res. Commun., 47, pp. 720-726 | ||
| 504 | |a Sakai, Cohen, (1976) Progress in Nucleic Acid Research and Molecular Biology, 17, pp. 15-42. , W.E. Cohn, Academic Press, New York | ||
| 504 | |a Bolton, Kearns, (1977) Biochim. Biophys. Acta, 477, pp. 10-19 | ||
| 504 | |a Scraba, Bradley, Leynitz-Willis, Warren, (1983) Virology, 124, pp. 152-160 | ||
| 520 | 3 | |a In order to study the intracellular polyamine distribution in Escherichia coli, 13C-NMR spectra of [1,4-13C]putrescine were obtained after addition of the latter to intact bacteria. The 13C-enriched methylene signal underwent line broadening. When the cells were centrifuged after 90 min the cell-bound putrescine peak had a linewidth of 23 Hz, while the supernatant liquid showed an unbound putrescine signal with a linewidth smaller than 1 Hz. By using 13C-enriched internal standards it could be shown that the linewidening was not due to the heterogeneity of the medium or to an in vivo paramagnetic effect. Cell-bound putrescine was liberated by addition of trichloroacetic acid and was therefore non-covalently linked to macromolecular cell structures. Cell-bound [13C]putrescine could be displaced by addition of an excess of [12C]putrescine. When samples of membranes, soluble protein, DNA, tRNA and ribosomes from E. coli were incubated with [1,4-13C]putrescine, strong binding was detected only in the ribosomal and membrane fractions. The ribosome-putrescine complex showed properties similar to those determined with the intact cells. By measuring the nuclear Overhauser enhancements η, it was possible to estimate that only about 50% of the polyamine was linked to the macromolecules. Determination of the T1 values of free and ribosomal-bound putrescine allowed the calculation of a correlation time, τc = 4·10-7 s for the latter. T1 and τc value for the ribosome-putrescine complex were those expected for a motional regime of slowly tumbling molecules. © 1984. |l eng | |
| 593 | |a Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín, 956 Buenos Aires, Argentina | ||
| 593 | |a Instituto de Investigaciones Bioquímicas 'Fundacion Campomar', Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Antonio Machado, 151 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a (E. COLI) |
| 690 | 1 | 0 | |a 13C-NMR |
| 690 | 1 | 0 | |a PUTRESCINE DISTRIBUTION |
| 690 | 1 | 0 | |a BACTERIAL DNA |
| 690 | 1 | 0 | |a PUTRESCINE |
| 690 | 1 | 0 | |a TRANSFER RNA |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a METHODOLOGY |
| 690 | 1 | 0 | |a NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY |
| 690 | 1 | 0 | |a RIBOSOME |
| 690 | 1 | 0 | |a CARBON ISOTOPES |
| 690 | 1 | 0 | |a DNA, BACTERIAL |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a MAGNETIC RESONANCE SPECTROSCOPY |
| 690 | 1 | 0 | |a PUTRESCINE |
| 690 | 1 | 0 | |a RIBOSOMES |
| 690 | 1 | 0 | |a RNA, TRANSFER |
| 650 | 1 | 7 | |2 spines |a CARBON |
| 700 | 1 | |a Frydman, R.B. | |
| 700 | 1 | |a de Los Santos, C. | |
| 700 | 1 | |a Garrido, D.A. | |
| 700 | 1 | |a Goldemberg, S.H. | |
| 700 | 1 | |a Algranati, I.D. | |
| 773 | 0 | |d 1984 |g v. 805 |h pp. 337-344 |k n. 4 |p Biochim. Biophys. Acta Mol. Cell Res. |x 01674889 |w (AR-BaUEN)CENRE-2263 |t BBA - Molecular Cell Research | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0167-4889(84)90016-8 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01674889_v805_n4_p337_Frydman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v805_n4_p337_Frydman |y Registro en la Biblioteca Digital |
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| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
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