The NADPH-cytochrome P450 reductase family in Trypanosoma cruzi is involved in the sterol biosynthesis pathway

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Trypanosoma cruzi flavoproteins TcCPR-A, TcCPR-B and TcCPR-C are members of the NADPH-dependent cytochrome P-450 reductase family expressed in the parasite. Epimastigotes over-expressing TcCPR-B and TcCPR-C showed enhanced ergosterol biosynthesis and increased NADP+/NADPH ratio. Transgenic parasites...

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Autor principal: De Vas, M.G
Otros Autores: Portal, P., Alonso, G.D, Schlesinger, M., Flawiá, M.M, Torres, H.N, Villamil, S.F, Paveto, C.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2011
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 Molecular Sequence Numbers  |a GENBANK: ABI15738, ABJ09678, ABJ09679; 
024 7 |2 cas  |a reduced nicotinamide adenine dinucleotide phosphate ferrihemoprotein reductase, 9023-03-4; NADP, 53-59-8; NADPH-Ferrihemoprotein Reductase, 1.6.2.4; Sterols 
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100 1 |a De Vas, M.G. 
245 1 4 |a The NADPH-cytochrome P450 reductase family in Trypanosoma cruzi is involved in the sterol biosynthesis pathway 
260 |c 2011 
270 1 0 |m Paveto, C.; Instituto de Investigaciones en Ingeniería, Genética y Biología Molecular (INGEBI) CONICET, Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina; email: cpaveto@dna.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a Trypanosoma cruzi flavoproteins TcCPR-A, TcCPR-B and TcCPR-C are members of the NADPH-dependent cytochrome P-450 reductase family expressed in the parasite. Epimastigotes over-expressing TcCPR-B and TcCPR-C showed enhanced ergosterol biosynthesis and increased NADP+/NADPH ratio. Transgenic parasites with augmented ergosterol content presented a higher membrane order with a corresponding diminished bulk-phase endocytosis. These results support a significant role for TcCPR-B and TcCPR-C in the sterol biosynthetic pathway and to our knowledge for the first time reveals the participation of more than one CPR in this metabolic route. Notably, TcCPR-B was found in reservosomes while TcCPR-C localised in the endoplasmic reticulum. In addition, we suggest a different role for TcCPR-A, since its over-expression is lethal, displaying cells with an increased DNA content, aberrant morphology and severe ultrastructural alterations. © 2010 Australian Society for Parasitology Inc.  |l eng 
536 |a Detalles de la financiación: Umweltbundesamt 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: We thank Dr. Fred Opperdoes (Universite Catholique de Louvain, Brussels) for the anti-GAPDH antibody, James Bangs (University of Wisconsin, USA) for the anti-BIP antibody, Dr. Carlos Labriola (Fundación Instituto LF Leloir, Argentina) for the anti-calreticulin antibody and Dr. Juan J. Cazzulo (UNSAM, Argentina) for the anti-cruzipain antibody. We also are grateful to Dr. Sandra Verstraeten for the GP value determinations. This work was funded by Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Universidad de Buenos Aires, (UBA) and Agencia Nacional de Promocion Cientifica y Tecnologica, (ANPCyT) from Argentina. 
593 |a Instituto de Investigaciones en Ingeniería, Genética y Biología Molecular (INGEBI) CONICET, Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina 
593 |a Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a INSERM U969, CNRS UMR7622, Université Pierre et Marie Curie, 9 quai Saint Bernard, Case 24, 75252 Paris, Cedex05, France 
690 1 0 |a NADPH-DEPENDENT CYTOCHROME P450 REDUCTASES 
690 1 0 |a STEROL METABOLISM 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a GENOMIC DNA 
690 1 0 |a REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE 
690 1 0 |a CYTOCHROME 
690 1 0 |a ENZYME ACTIVITY 
690 1 0 |a GENE EXPRESSION 
690 1 0 |a METABOLISM 
690 1 0 |a PARASITE 
690 1 0 |a STEROL 
690 1 0 |a ARTICLE 
690 1 0 |a CELLULAR DISTRIBUTION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DNA CONTENT 
690 1 0 |a ENDOCYTOSIS 
690 1 0 |a ENDOPLASMIC RETICULUM 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a GENE OVEREXPRESSION 
690 1 0 |a IMMUNOFLUORESCENCE MICROSCOPY 
690 1 0 |a MEMBRANE FLUIDITY 
690 1 0 |a MEMBRANE PERMEABILITY 
690 1 0 |a MORPHOLOGY 
690 1 0 |a NONHUMAN 
690 1 0 |a NUCLEOTIDE SEQUENCE 
690 1 0 |a OXIDATION REDUCTION STATE 
690 1 0 |a PROTEIN DETERMINATION 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a PROTEIN FUNCTION 
690 1 0 |a STEROL SYNTHESIS 
690 1 0 |a TRANSGENE 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a ANIMALS 
690 1 0 |a BIOSYNTHETIC PATHWAYS 
690 1 0 |a CELL MEMBRANE 
690 1 0 |a GENE EXPRESSION 
690 1 0 |a NADP 
690 1 0 |a NADPH-FERRIHEMOPROTEIN REDUCTASE 
690 1 0 |a ORGANELLES 
690 1 0 |a PHAGOCYTOSIS 
690 1 0 |a STEROLS 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a TRYPANOSOMA CRUZI 
700 1 |a Portal, P. 
700 1 |a Alonso, G.D. 
700 1 |a Schlesinger, M. 
700 1 |a Flawiá, M.M. 
700 1 |a Torres, H.N. 
700 1 |a Villamil, S.F. 
700 1 |a Paveto, C. 
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