Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1985
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 10112caa a22012137a 4500 | ||
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| 001 | PAPER-1053 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203025.0 | ||
| 008 | 190411s1985 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0022268757 | |
| 024 | 7 | |2 cas |a adenosine phosphate, 61-19-8, 8063-98-7; calcium, 7440-70-2; 3',5' cyclic nucleotide phosphodiesterase, 9040-59-9; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Calcium, 7440-70-2; Calmodulin | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MBIPD | ||
| 100 | 1 | |a Téllez-iñón, M.T. | |
| 245 | 1 | 0 | |a Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| 260 | |c 1985 | ||
| 270 | 1 | 0 | |m Torres, H.N.; Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Instituto de Investigaciones Bioquímicas, Facultad de Ciencías Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Klee, Krinks, Purification of cyclic 3′,5′ nucleotide phosphodiesterase inhibitory protein by affinity chromatography on activator protein coupled to Sepharose (1978) Biochemistry, 17, pp. 120-126 | ||
| 504 | |a Jamieson, Vanaman, Calcium-dependent affinity chromatography of calmodulin on an immobilized phenotiazine (1979) Biochem. Biophys. Res. Commun., 90, pp. 1048-1055 | ||
| 504 | |a Bazari, Clarke, Characterization of a novel calmodulin from Dictyostelium discoideum (1981) J. Biol. Chem., 256, pp. 3598-3603 | ||
| 504 | |a Reig, Téllez-Iñón, Flawiá, Torres, Activation of Neurospora crassa soluble adenylate cyclase by calmodulin (1984) Biochem. J., 221, pp. 541-543 | ||
| 504 | |a Thompson, Appleman, Multiple cyclic nucleotide phosphodiesterase activities from rat brain (1971) Biochemistry, 10, pp. 311-316 | ||
| 504 | |a Londesborough, Quantitative estimation of 3′,5′ cyclic AMP phosphodiesterase using anion exchange resin in a batch process (1976) Anal. Biochem., 71, pp. 623-628 | ||
| 504 | |a Téllez-Iñón, Glikin, Torres, Cyclic nucleotide phosphodiesterase in N crassa (1982) Biochem J, 203, pp. 611-616 | ||
| 504 | |a Clarke, Bazari, Kayman, Isolation and properties of calmodulin from Dictyostelium discoideum (1980) J. Bacteriology, 141, pp. 397-400 | ||
| 504 | |a Lowry, Rosenborough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Kornblihtt, Flawiá, Torres, Manganese ion dependent adenylate cyclase activity in rat testis: purification and properties (1981) Biochemistry, 20, pp. 1262-1267 | ||
| 504 | |a Reig, Kornblihtt, Flawiá, Torres, Soluble adenylate cyclase in Neurospora crassa (1982) Biochem. J., 207, pp. 43-49 | ||
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| 504 | |a Gietzen, Adamczyk-Engelmann, Wuthrich, Konstantinova, Bader, Compound 48/80 is a selective and powerful inhibitor of calmodulin-regulated functions (1983) Biochim. Biophys. Acta, 736, pp. 109-118 | ||
| 504 | |a Strada, Martin, Thompson, General properties of multiple molecular forms of cyclic nucleotide phosphodiesterase in the nervous system (1984) Adv. Cyclic Nucl. Res., 16, pp. 13-29 | ||
| 504 | |a Ortega-Pérez, Van Tuinen, Marmé, Cox, Turian, Purification and identification of calmodulin from N. crassa (1981) FEBS Lett., 133, pp. 205-208 | ||
| 504 | |a Kakiuchi, Sobue, Yamazaki, Nagao, Umeki, Nozawa, Yazawa, Yagi, Ca2+-dependent modulator proteins from Tetrahymena pyriformis, sea anemone and scallops and guanylate cyclase activation (1981) J. Biol. Chem., 256, pp. 19-22 | ||
| 504 | |a Satir, Garofalo, Gilligan, Maihle, Possible functions of calmodulin in Protozoa (1980) Annals of the New York Academy of Sciences, 336, pp. 83-91 | ||
| 504 | |a Ruben, Egwuagu, Patton, African Trypanosomes contain calmodulin which is distinct from host calmodulin (1983) Biochim. Biophys. Acta, 758, pp. 104-113 | ||
| 504 | |a Strickler, Patton, Adenosine 3′,5′ monophosphate in reproducing and differentiated trypanosomes (1975) Science, 190, pp. 1110-1111 | ||
| 504 | |a Pearson, Marian, Harcus, Hall, Hewlett, Lethal effect of phenotiazine neuroleptics on the pathogenic protozoan Leishmania donovani (1982) Science, 217, pp. 369-370 | ||
| 504 | |a Bartfai, Preparations of metal-chelate complexes and the design of steady-state kinetics experiments involving metal nucleotide complexes (1979) Adv. Cyclic Nucl. Res., 10, pp. 219-242 | ||
| 520 | 3 | |a Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band with an apparent molecular weight of 16 000. In addition, cyclic AMP phosphodiesterase activity from T. cruzi epimastigote forms was purified by ion-exchange chromatography and affinity chromatography on a brain calmodulin-Sepharose column. The enzyme was activated by homologous calmodulin as well as by bovine brain and Neurospora crassa calmodulins. The activation required micromolar concentrations of Ca2+ and it was blocked by EGTA and by some neuroleptic drugs such as chlorpromazine, fluphenazine and compound 48 80. Activations were observed at micromolar concentrations of cyclic AMP as substrate. In addition, T. cruzi calmodulin was also active in bringing about the stimulation of brain phosphodiesterase. © 1985. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: The authors are indebted to Drs. Elsa L. Segura and Juan J. Cazzulo, from the Instituto Nacional de Diagn6stico e Investigaci6n de la Enfermedad de Chagas 'Dr. Mario F. Chab6n', Ministerio de Salud y Acci6n Social, Buenos Aires, for providing T. cruzi cultures. M.T.T.-I., M.T. and H.N.T, are Career Investigators from the Consejo Nacional de Investigaciones Cientificas y T6cnicas (CONICET) and R.M.U. is a Research Fellow of CONICET. | ||
| 593 | |a Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Instituto de Investigaciones Bioquímicas, Facultad de Ciencías Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a PHOSPHODIESTERASE |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ADENOSINE PHOSPHATE |
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a ENZYME |
| 690 | 1 | 0 | |a PHOSPHODIESTERASE |
| 690 | 1 | 0 | |a 3',5' CYCLIC NUCLEOTIDE PHOSPHODIESTERASE |
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTOZOON |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BRAIN CORTEX |
| 690 | 1 | 0 | |a CATTLE |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a 3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a CATTLE |
| 690 | 1 | 0 | |a CEREBRAL CORTEX |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Ulloa, R.M. | |
| 700 | 1 | |a Torruella, M. | |
| 700 | 1 | |a Torres, H.N. | |
| 773 | 0 | |d 1985 |g v. 17 |h pp. 143-153 |k n. 2 |p Mol. Biochem. Parasitol. |x 01666851 |w (AR-BaUEN)CENRE-6136 |t Molecular and Biochemical Parasitology | |
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