Oxidizing substrate specificity of Mycobacterium tuberculosis alkyl hydroperoxide reductase E: Kinetics and mechanisms of oxidation and overoxidation
Alkyl hydroperoxide reductase E (AhpE), a novel subgroup of the peroxiredoxin family, comprises Mycobacterium tuberculosis AhpE (MtAhpE) and AhpE-like proteins present in many bacteria and archaea, for which functional characterization is scarce. We previously reported that MtAhpE reacted ~ 10 3 tim...
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2011
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| 024 | 7 | |2 cas |a alcohol, 64-17-5; arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; peroxiredoxin, 207137-51-7; Peroxiredoxins, 1.11.1.15 | |
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| 100 | 1 | |a Reyes, A.M. | |
| 245 | 1 | 0 | |a Oxidizing substrate specificity of Mycobacterium tuberculosis alkyl hydroperoxide reductase E: Kinetics and mechanisms of oxidation and overoxidation |
| 260 | |c 2011 | ||
| 270 | 1 | 0 | |m Trujillo, M.; Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Universidad de la República, General Flores 2125, 11800 Montevideo, Uruguay; email: madiat@fmed.edu.uy |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Alkyl hydroperoxide reductase E (AhpE), a novel subgroup of the peroxiredoxin family, comprises Mycobacterium tuberculosis AhpE (MtAhpE) and AhpE-like proteins present in many bacteria and archaea, for which functional characterization is scarce. We previously reported that MtAhpE reacted ~ 10 3 times faster with peroxynitrite than with hydrogen peroxide, but the molecular reasons for that remained unknown. Herein, we investigated the oxidizing substrate specificity and the oxidative inactivation of the enzyme. In most cases, both peroxidatic thiol oxidation and sulfenic acid overoxidation followed a trend in which those peroxides with the lower leaving-group pK a reacted faster than others. These data are in agreement with the accepted mechanisms of thiol oxidation and support that overoxidation occurs through sulfenate anion reaction with the protonated peroxide. However, MtAhpE oxidation and overoxidation by fatty acid-derived hydroperoxides (~ 10 8 and 10 5 M - 1 s - 1, respectively, at pH 7.4 and 25 °C) were much faster than expected according to the Brønsted relationship with leaving-group pK a. A stoichiometric reduction of the arachidonic acid hydroperoxide 15-HpETE to its corresponding alcohol was confirmed. Interactions of fatty acid hydroperoxides with a hydrophobic groove present on the reduced MtAhpE surface could be the basis of their surprisingly fast reactivity. © 2011 Elsevier Inc. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Investigación e Innovación, FCE_516 | ||
| 536 | |a Detalles de la financiación: The authors thank ANII for financial support (FCE_516) to A.T. A.R. and M.H. were partially supported by fellowships from ANII. We also thank Marcelo Martí and Darío Estrín (Departamento de Química Inorgánica, Analítica y Química Física/INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina) for helpful discussion. R.R. is a Howard Hughes Medical Institute Research Scholar. Appendix A | ||
| 593 | |a Departamento de Bioquímica, Universidad de la República, 11800 Montevideo, Uruguay | ||
| 593 | |a Center for Free Radical and Biomedical Research, Universidad de la República, 11800 Montevideo, Uruguay | ||
| 593 | |a Departamento de Química Inorgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Universidad de la República, General Flores 2125, 11800 Montevideo, Uruguay | ||
| 690 | 1 | 0 | |a ALKYL HYDROPEROXIDE REDUCTASE E |
| 690 | 1 | 0 | |a ARACHIDONIC ACID |
| 690 | 1 | 0 | |a FREE RADICALS |
| 690 | 1 | 0 | |a HYDROPEROXIDE |
| 690 | 1 | 0 | |a MYCOBACTERIUM TUBERCULOSIS |
| 690 | 1 | 0 | |a PEROXIDASE |
| 690 | 1 | 0 | |a PEROXIREDOXIN |
| 690 | 1 | 0 | |a ALCOHOL |
| 690 | 1 | 0 | |a ALKYL HYDROPEROXIDE REDUCTASE E |
| 690 | 1 | 0 | |a ARACHIDONIC ACID |
| 690 | 1 | 0 | |a HYDROPEROXIDE |
| 690 | 1 | 0 | |a PEROXIREDOXIN |
| 690 | 1 | 0 | |a SULFENIC ACID DERIVATIVE |
| 690 | 1 | 0 | |a THIOL |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ENZYME INACTIVATION |
| 690 | 1 | 0 | |a ENZYME SPECIFICITY |
| 690 | 1 | 0 | |a HYDROPHOBICITY |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MYCOBACTERIUM TUBERCULOSIS |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OXIDATION |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTON TRANSPORT |
| 690 | 1 | 0 | |a STOICHIOMETRY |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, LIQUID |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MYCOBACTERIUM TUBERCULOSIS |
| 690 | 1 | 0 | |a OXIDATION-REDUCTION |
| 690 | 1 | 0 | |a PEROXIREDOXINS |
| 690 | 1 | 0 | |a SUBSTRATE SPECIFICITY |
| 690 | 1 | 0 | |a TANDEM MASS SPECTROMETRY |
| 690 | 1 | 0 | |a MYCOBACTERIUM TUBERCULOSIS |
| 700 | 1 | |a Hugo, M. | |
| 700 | 1 | |a Trostchansky, A. | |
| 700 | 1 | |a Capece, L. | |
| 700 | 1 | |a Radi, R. | |
| 700 | 1 | |a Trujillo, M. | |
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