Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by v...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1985
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 05685caa a22007697a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1021 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203023.0 | ||
| 008 | 190411s1985 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0022253656 | |
| 024 | 7 | |2 cas |a 1,4 alpha glucan branching enzyme, 9001-97-2; 1,4-alpha-Glucan Branching Enzyme, EC 2.4.1.18; Glucans; Glucosyltransferases, EC 2.4.1.-; Polysaccharides | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ANBCA | ||
| 100 | 1 | |a Krisman, C.R. | |
| 245 | 1 | 0 | |a Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points |
| 260 | |c 1985 | ||
| 270 | 1 | 0 | |m Krisman, C.R.; Instituto de Investigaciones Bioquimicas Fundacion Campomar Antonio Machado 151, 1405 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Boyer, Preiss, Multiple forms of (1 → 4)-α-d-glucan, (1 → 4)-α-d-glucan-6- glycosyl transferase from developing zea mays L. Kernels (1978) Carbohydrate Research, 61, p. 321 | ||
| 504 | |a Brown, Brown, (1966) Methods in Enzymology, 8, p. 395. , E.F. Neufeld, V. Ginsburg, Academic Press, New York | ||
| 504 | |a Krisman, (1962) Biochem. Biophys. Acta, 65, p. 301 | ||
| 504 | |a Krisman, Kopun, (1982) Eur. J. Cell Biol, 27, p. 18 | ||
| 504 | |a Krisman, (1962) Anal. Biochem, 4, p. 17 | ||
| 504 | |a White, Nelson, Gillard, Zingaro, (1981) Mechanisms of Saccharide Polymerization and Depolymerization, p. 265. , J.J. Marshall, Academic Press, New York | ||
| 504 | |a Cowie, Greenwood, 559. Physicochemical studies on starches. Part VI. Aqueous leaching and the fractionation of potato starch (1957) Journal of the Chemical Society (Resumed), p. 2862 | ||
| 504 | |a Mordoh, Krisman, Leloir, (1966) Arch. Biochem. Biophys, 113, pp. 265-272 | ||
| 504 | |a Montgomery, (1957) Arch. Biochem. Biophys, 67, p. 378 | ||
| 504 | |a Ashwell, (1957) Methods in Enzymology, 3, p. 85. , S.P. Colowick, N.O. Kaplan, Academic Press, New York | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biochem. Chem, 193, p. 265 | ||
| 504 | |a Fales, (1959) Anal. Chem, 31, p. 1898 | ||
| 504 | |a Fisher, Krebs, (1962) Methods in Enzymology, 5, p. 369. , S.P. Colowick, N.O. Kaplan, Academic Press, New York | ||
| 504 | |a Brown, Brown, (1966) Methods in Enzymology, 7, p. 515. , S.P. Colowick, N.O. Kaplan, Academic Press, New York | ||
| 504 | |a Borovsky, Smith, Whelan, (1975) Eur. J. Biochem, 59, p. 615 | ||
| 504 | |a Leloir, National Cancer Institute Monograph No. 27 (1966) International Symposium on Enzymatic Aspects of Metabolic Regulation, pp. 3-18. , Mexico | ||
| 520 | 3 | |a Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by varying amounts of branching enzyme. The procedure involved the synthesis of a polysaccharide from Glc-1-P and phosphorylase in the presence of the sample to be tested. The branched polysaccharide was then purified and the glucoses involved in the branching points were quantitated after degradation with phosphorylase and debranching enzymes. This method appeared to be useful, not only in enzymatic activity determinations but also in the study of the structure of α-d-glucans when combined with those of total polysaccharide quantitation, such as iodine and phenol-sulfuric acid. © 1985. |l eng | |
| 536 | |a Detalles de la financiación: National Research Council | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: ’ Member of the Research Career of the National Research Council (CONICET), Argentina. ’ Recipient of the Fellowship “Maria Scasso de Cam-pomar.” 3 Member of the Technical Career of the National Research Council (CONICET), Argentina. | ||
| 593 | |a Instituto de Investigaciones Bioquimicas Fundacion Campomar Antonio Machado 151, 1405 Buenos Aires, Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales, Antonio Machado 151, 1405 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a AMYLO-Α1,4-Α1,6-TRANSGLUCOSYLASE |
| 690 | 1 | 0 | |a AMYLOPECTIN |
| 690 | 1 | 0 | |a BRANCHING ENZYME ASSAY |
| 690 | 1 | 0 | |a GLYCOGEN |
| 690 | 1 | 0 | |a QUANTITATION OF BRANCHING POINTS |
| 690 | 1 | 0 | |a Α1,6-GLUCOSYDIC LINKAGE |
| 690 | 1 | 0 | |a 1,4 ALPHA GLUCAN BRANCHING ENZYME |
| 690 | 1 | 0 | |a ENZYME ASSAY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a 1,4-ALPHA-GLUCAN BRANCHING ENZYME |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a GLUCANS |
| 690 | 1 | 0 | |a GLUCOSYLTRANSFERASES |
| 690 | 1 | 0 | |a POLYSACCHARIDES |
| 690 | 1 | 0 | |a RABBITS |
| 700 | 1 | |a Tolmasky, D.S. | |
| 700 | 1 | |a Raffo, S. | |
| 773 | 0 | |d 1985 |g v. 147 |h pp. 491-496 |k n. 2 |p Anal. Biochem. |x 00032697 |w (AR-BaUEN)CENRE-163 |t Analytical Biochemistry | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022253656&doi=10.1016%2f0003-2697%2885%2990303-3&partnerID=40&md5=47daa5c34b1b9cbd085f160f0f8a99f9 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0003-2697(85)90303-3 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v147_n2_p491_Krisman |y Registro en la Biblioteca Digital |
| 961 | |a paper_00032697_v147_n2_p491_Krisman |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 61974 | ||