In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1986
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 06771caa a22008177a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1006 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203022.0 | ||
| 008 | 190411s1986 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0022648683 | |
| 024 | 7 | |2 cas |a acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; lipid, 66455-18-3; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; Lipids; Oligosaccharides; Pyruvates; Pyruvic Acid, 127-17-3 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBRCA | ||
| 100 | 1 | |a Bossio, J.C. | |
| 245 | 1 | 3 | |a In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii |
| 260 | |c 1986 | ||
| 270 | 1 | 0 | |m Bossio, J.C.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales (UBA), 1405 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Bauer, Infection of Legumes by Rhizobia (1981) Annual Review of Plant Physiology, 32, pp. 407-449 | ||
| 504 | |a Albersheim, Darvill, Davis, Lau, McNeil, Sharp, York, (1984) Structure, Function and Biosynthesis of Plant Cell Walls, pp. 19-51. , W.M. Dugger, S. Bartnicki-García | ||
| 504 | |a Tolmasky, Staneloni, Ugalde, Leloir, (1980) Arch. Biochem. Biophys, 203, pp. 358-364 | ||
| 504 | |a Tolmasky, Staneloni, Leloir, (1982) J. Biol. Chem, 257, pp. 6751-6757 | ||
| 504 | |a Staneloni, Tolmasky, Leloir, (1984) J. Gen. Microbiol, 130, pp. 869-879 | ||
| 504 | |a Robertsen, Aman, Darvill, McNeil, Albersheim, Host-Symbiont Interactions : V. THE STRUCTURE OF ACIDIC EXTRACELLULAR POLYSACCHARIDES SECRETED BY RHIZOBIUM LEGUMINOSARUM AND RHIZOBIUM TRIFOLII (1981) PLANT PHYSIOLOGY, 67, pp. 389-400 | ||
| 504 | |a Couso, Ielpi, Dankert, (1980) Arch. Biochem. Biophys, 204, pp. 434-443 | ||
| 504 | |a GarcÃa, Recondo, Dankert, Polysaccharide Biosynthesis in Acetobacter xylinum. Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars (1974) European Journal of Biochemistry, 43, pp. 93-105 | ||
| 504 | |a Couso, Ielpi, García, Dankert, (1982) Eur. J. Biochem, 123, pp. 617-627 | ||
| 504 | |a Ielpi, Couso, Dankert, (1981) Biochem. Biophys. Res. Commun, 102, pp. 1400-1408 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem, 193, pp. 265-275 | ||
| 504 | |a Pont Lezica, R., Brett, C.T., Romero, P. and Dankert, M.A. Biochem. Biophys. Res. Commun. 66, 980-987; Trevelyan, Procter, Harrison, (1950) Nature (London), 166, pp. 444-445 | ||
| 504 | |a Amemura, Harada, (1983) Carbohydr. Res, 112, pp. 85-93 | ||
| 504 | |a Troy, Furman, Heath, (1971) J. Biol. Chem, 246, pp. 118-133 | ||
| 504 | |a Ielpi, Couso, Dankert, (1981) FEBS Lett, 130, pp. 253-256 | ||
| 504 | |a Ielpi, Couso, Dankert, (1983) Biochem. Internat, 6, pp. 323-333 | ||
| 504 | |a Chaudhari, Bishop, Dudman, (1973) Carbohydr. Res, 28, pp. 221-231 | ||
| 504 | |a Jansson, Lindberg, Ljunagren, (1979) Carbohydr. Res, 75, pp. 207-220 | ||
| 520 | 3 | |a EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis of these extracts produce cyclic phosphate esters suggesting the presence of a diphosphate bridge, and mild acid or catalytic reduction-alkaline phosphatase treatments release four main componenets a, b, c and d, as judged by paper electrophoresis and chromatography and gel filtration studies. The four components can be obtained (14C)acetyl-labeled, but only compound c and to a lesser degree compound b can be (14C)pyruvate-labeled. For the exopolysaccharide produced by this strain the following repeating unit has been proposed (Robertsen et al. (1981), Plant Physiol. 67, 389-400): {A figure is presented}. The results obtained suggest that the octasaccharide repeating unit (compound a) with one (compound b) or two (compound c) ketal pyruvate residues are assembled on a lipid acceptor. All these compounds are assumed to be intermediates in the biosynthesis of R. trifolii exopolysaccharide. © 1986. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, 435 | ||
| 536 | |a Detalles de la financiación: Facultad de Ciencias Físicas y Matemáticas | ||
| 536 | |a Detalles de la financiación: Instituto de Investigaciones Biecas "Fundaci6n C-r", Facultad de Ciencias Exactas y Naturales (DBA) and Consejo National de Investigaciones Cientificas y T&nicas Patricias Argentinas 435, (1405) Buenos Aires, Argentina | ||
| 536 | |a Detalles de la financiación: Am.-The authors are indebted to Dr I. Algranati and J.M. Olavarrla for critically reading this nmnuscript. They also thank the excellent technical assitance of Misses. Susana Raffo and Marta Bravo in the preparation of labeled sugar nucleotides. M.A.D. is a Career Investigator of the Consejo National de Investigaciones Cientlficas y T&nicas (Argentina). | ||
| 593 | |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales (UBA), 1405 Buenos Aires, Argentina | ||
| 593 | |a Consejo Nacional de Investigaciones Cientificas y Técnicas Patricias Argentinas 435, 1405 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ACETIC ACID |
| 690 | 1 | 0 | |a LIPID |
| 690 | 1 | 0 | |a OLIGOSACCHARIDE |
| 690 | 1 | 0 | |a PYRUVIC ACID |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RHIZOBIUM TRIFOLII |
| 690 | 1 | 0 | |a ACETYLATION |
| 690 | 1 | 0 | |a CARBOHYDRATE SEQUENCE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, GEL |
| 690 | 1 | 0 | |a ELECTROPHORESIS, PAPER |
| 690 | 1 | 0 | |a LIPIDS |
| 690 | 1 | 0 | |a OLIGOSACCHARIDES |
| 690 | 1 | 0 | |a PYRUVATES |
| 690 | 1 | 0 | |a PYRUVIC ACID |
| 690 | 1 | 0 | |a RHIZOBIUM |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 700 | 1 | |a Inon de Iannino, N. | |
| 700 | 1 | |a Dankert, M.A. | |
| 773 | 0 | |d 1986 |g v. 134 |h pp. 205-211 |k n. 1 |p Biochem. Biophys. Res. Commun. |x 0006291X |w (AR-BaUEN)CENRE-905 |t Biochemical and Biophysical Research Communications | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0006-291X(86)90548-6 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v134_n1_p205_Bossio |y Registro en la Biblioteca Digital |
| 961 | |a paper_0006291X_v134_n1_p205_Bossio |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 61959 | ||