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03081cam a22006737a 4500 |
| 001 |
BIBLO-46427 |
| 003 |
AR-BaUEN |
| 005 |
20201111150531.0 |
| 008 |
130304s2012 ne ||||f |||| 00| 0|eng|d |
| 040 |
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|a AR-BaUEN
|b spa
|c AR-BaUEN
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| 020 |
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|a 9780123855442
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| 044 |
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|a ne
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| 080 |
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|a 577.2
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| 100 |
1 |
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|a Carson, Susan
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| 245 |
1 |
0 |
|a Molecular biology techniques :
|b a classroom laboratory manual
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| 250 |
|
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|a 3rd. ed.
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| 260 |
|
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|a Amsterdam :
|b Elsevier,
|c 2012
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| 300 |
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|a xxi, 200 p. + gráf.
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| 505 |
0 |
0 |
|t Preface
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| 505 |
0 |
0 |
|t About the Authors
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| 505 |
0 |
0 |
|t Acknowledgements
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| 505 |
0 |
0 |
|t Note to Instructors
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| 505 |
0 |
0 |
|t Instrumentation
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| 505 |
0 |
0 |
|t Nomenclature
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| 505 |
0 |
0 |
|t Introduction
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| 505 |
0 |
0 |
|g Lab Session 1
|t Getting Oriented: Practicing with Micropipettes
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| 505 |
0 |
0 |
|g Lab Session 2
|t Purification and Digestion of Plasmid (Vector) DNA
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| 505 |
0 |
0 |
|g Lab Session 3
|t PCR Amplification of egfp and Completion of Vector Preparation
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| 505 |
0 |
0 |
|g Lab Session 4
|t Preparation of Insert DNA (egfp) PCR Product
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| 505 |
0 |
0 |
|g Lab Session 5
|t DNA Ligation and Transformation of Escherichia coli
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| 505 |
0 |
0 |
|g Lab Session 6
|t Colony Hybridization
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| 505 |
0 |
0 |
|g Lab Session 6A
|t Interim Laboratory Session
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| 505 |
0 |
0 |
|g Lab Session 6B
|t Colony Hibridization: Monoclonal Antibody Probe
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| 505 |
0 |
0 |
|g Lab Session 6
|t Discussion Questions
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| 505 |
0 |
0 |
|g Lab Session 7
|t Characterization of Recombinant Clones: Part 1
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| 505 |
0 |
0 |
|g Lab Session 8
|t Characterization of Recombinant Clones: Part 2
|
| 505 |
0 |
0 |
|g Lab Session 9
|t Characterization of Recombinant Clones: Part 3
|
| 505 |
0 |
0 |
|g Lab Session 10
|t Computational Analysis of DNA Sequence from a Positive Clone: Part 2
|
| 505 |
0 |
0 |
|g Lab Session 11
|t Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1
|
| 505 |
0 |
0 |
|g Lab Session 12
|t Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2
|
| 505 |
0 |
0 |
|g Lab Session 13
|t Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column
|
| 505 |
0 |
0 |
|g Lab Session 14
|t Analysis of Purification Fractions
|
| 505 |
0 |
0 |
|g Lab Session 15
|t Total RNA Purification
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| 505 |
0 |
0 |
|g Lab Session 16
|t Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1
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| 505 |
0 |
0 |
|g Lab Session 17
|t Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2
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| 505 |
0 |
0 |
|g Lab Session 18
|t Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1
|
| 505 |
0 |
0 |
|g Lab Session 19
|t Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2
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| 505 |
0 |
0 |
|g Appendix 1
|t Equipment
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| 505 |
0 |
0 |
|g Appendix 2
|t Prep List
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| 505 |
0 |
0 |
|g Appendix 3
|t Preparation of Competent E. coli Cells
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| 505 |
0 |
0 |
|g Appendix 4
|t Pre-Lab Questions
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| 505 |
0 |
0 |
|t Index
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| 653 |
1 |
0 |
|a ADN
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| 653 |
1 |
0 |
|a PROTEINAS RECOMBINANTES
|
| 650 |
1 |
7 |
|2 spines
|a BIOLOGIA MOLECULAR
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| 650 |
1 |
4 |
|a ENSAYOS DE LABORATORIOS
|
| 700 |
1 |
|
|a Miller, Heather B.
|
| 700 |
1 |
|
|a Witherow, D. Scott
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| 962 |
|
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|a info:eu-repo/semantics/book
|a info:ar-repo/semantics/libro
|b info:eu-repo/semantics/publishedVersion
|